Posted by admin on Jul 31, 2014 in |
The anthrone method has been employed for many years in the spectrophotometric determination of polysaccharides. However, taking into account that its use usually involves a series of steps and requires a long period of analysis, this study aimed to conduct a more detailed assessment of its application in the determination of total polysaccharides from the aerial parts of Thuja occidentalis Linn. Thus, this study attempted to optimize and validate a viable method for this species, whose antimetastatic and immunostimulant properties have been attributed to the metabolites analyzed in this study. The optimized and validated method showed satisfactory results, and this method lasts about 6 hours, in contrast to the diverse methodologies reported in the literature with an estimated duration of 41 hours. Thus, the presented method demonstrated to be a viable tool for the quality control of the plant drug and products derived from the aerial parts of T....
Read More
Posted by admin on Jul 31, 2014 in |
Probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit. There are very limited studies on antidiabetic effect of lactic acid bacteria despite their potentiality in reducing the risk of diabetes onset. The present study explored the effect of administering live culture of L. rhamnosus [MTCC No 8712] and L. casei [MTCC No 1408] in STZ induced diabetic albino male rats. Animals (approximately aged 6 months) were grouped into three in a randomized fashion that average weight of animals in each group would be 150-250 gms. Normal feed pellets and water ad libitum were provided to animals throughout the experiment. As Group 1-Control Group, Group 2-Positive control Group were on only insulin treatment (1 unit /day) and the Group 3-Treated Group were on probiotics treatment.The treatment was continued for 30days. Body weight, blood glucose, lipid profile, antioxidative status was analyzed by standard protocol. After subculturing L. rhamnosus and L. casei and standardizing with Mac Ferlan’s device, the mixture culture was administered to the treatment group...
Read More
Posted by admin on Jul 31, 2014 in |
This experimental work present the development and validation of a simple, rapid, accurate and precise RP-HPLC method for the simultaneous estimation of amlodipine besylate (AMB) and nebivolol hydrochloride (NBH) in bulk drug and pharmaceutical dosage forms. The chromatographic separation was carried out with AGILENT 1120 liquid chromatograph in an isocratic mode using Kromasil ODS column (250 x 4.6mm x 5µ particle size) with a mobile phase of mixed acetate Buffer pH 5: acetonitrile (60:40v/v) and the eluents were monitored at 268nm. The retention times of AMB and NBH were 5.26min and 6.84min respectively. The method was found to be linear over the concentration range of 5-25µg/ml for amlodipine besylate and 10-50µg/ml for nebivolol hydrochloride with a correlation coefficient of 0.999. The percentage recoveries of AMB and NBH were found to be 99.01-101.5 % and 99.2-101.0% respectively. The proposed RP-HPLC method was validated according to ICH guidelines and was employed for routine quality control analysis in bulk and combined dosage...
Read More
Posted by admin on Jul 31, 2014 in |
Phytochemical studies on the fruits of Phaleria macrocarpa yielded 2,6,4′-trihydroxy-4-methoxy-benzophenone (1) and 6,4′-dihydroxy-4-methoxybenzophenone-2-O–β-D-glucopyrano- side (2) together with two triterpenoids, 24-methylenecycloartan-3-one (3) and 24-methyl-9,19-cyclo- lanost-25-en-3-ol (4). Investigation on the leaves part led to the isolation of β-sitosterol (5) and stigmasterol (6). All extracts and isolated compounds were tested for their antibacterial activity using disc diffusion method followed by minimal inhibitory concentration (MIC) against two Gram-positive bacterial strains, Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 29737) and two Gram-negative bacterial strains, Escherichia coli (ATCC 10536) and Pseudomonas putida (ATCC 49128). In general, the result has demonstrated that all extracts and isolated compounds were exhibited weak activity against all tested bacteria with the inhibition zone having diameter between 6.1 – 7.3 mm.This is the first report on the isolation of triterpenoids (3 and 4) from this plant and the antibacterial activity for compounds...
Read More
Posted by admin on Jul 16, 2014 in |
Blepharis is a genus belonging to family Acanthaceae. It is an Afro-asiatic genus comprising 129 species which occur in arid and semi-arid habitats. They are used for the treatment of a number of ailments. Anti-inflammatory activity was performed based on the folk lore information. The present study is aimed at the evaluation ofanti-inflammatory property of the petroleum ether, ethyl acetate and ethanolic extracts of Blepharis maderaspatensis (L.) Heyne ex Roth. and Blepharis molluginifoliaPers. whole plantby using Human red blood cell (HRBC) membrane stabilization method. Ibuprofen is used as reference drug for comparison. HRBC method was selected for the in vitro evaluation of anti-inflammatory property because the erythrocyte membrane is analogous to the lysosomal membrane and its stabilization implies that the extract may as well stabilize lysosomal membranes. The results revealed that both Blepharis maderaspatensis (L.) Heyne ex Roth. and Blepharis molluginifoliaPers. have anti-inflammatory activity. Among the test samples tested for membrane stabilization, Blepharis maderaspatensis ethyl acetate extract showed better membrane stability with 323.3±15.7 IC50 (µg/ml), followed by with same...
Read More
