Posted by admin on Jul 1, 2014 in |
The different extracts of leaf and bark of the plant Holoptelia integrifolia were investigated for in-vitro anti-inflammatory activity by human red blood cell membrane stabilization (HRBC) method. The HRBC membrane stabilization activity of the petroleum ether extract of leaf at the concentration of 200 mg/ml showed 68.12+/-1.05 % and petroleum ether extract of bark at the concentration of 200 mg/ml showed 71.25+/-1.72 %. Inhibition of denaturation in hypotonic solution with standard Diclofenac sodium 100 mg/ml showed 79.25%. Therefore, these extracts showed equipotent activity to Diclofenac...
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Posted by admin on Jul 1, 2014 in |
Acacia catechu is a multipurpose tree predominantly found in tropical and subtropical regions of India. The tree produces nitrogen rich fodder and green manure, high quality fuel wood and charcoal, strong durable poles and timber. The most useful products of this tree are Kutch or “Kattha” which is obtained from bark and heartwood of the tree. Commonly used plant part of Acacia catechu is bark which is a usual source of Kattha, is strong antioxidant, astringent, anti-inflammatory, antibacterial and antifungal in nature. The tree is also used as fodder for goat and cattle in many parts of India. However, the other plant parts of Acacia catechu especially from Central India have not been investigated for their nutritive value. The present study showcases a comprehensive investigation on different plant parts of Acacia catechu collected from Jabalpur. The results show that the young legumes of Acacia catechu can serve as a better source of nutrition for both human and...
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Posted by admin on Jul 1, 2014 in |
This communication reports on an easy and cost effective mycological medium, developed for primary isolation and culture of lipophilic yeasts. The medium has been modified and adjusted specifically to promote the growth of lipophilic yeasts and simultaneously suppress the growth of other fungi and bacteria. The traditional medium of Sabouraud’s dextrose agar (SDA) with olive oil overlay was modified by incorporating different oils (coconut, palm, corn, olive and sesame) in the medium. The oil incorporated SDA medium was compared with other routinely used media such as Modified Dixon’s, IMU – Mf and Modified Leeming – Notman, for the culture of lipophilic yeasts. From the results obtained, it was observed that the medium with sesame oil exhibited the highest viable count within 48 hours. Thus, this medium can be used in the conventional microbial procedures such as isolation of pure culture by streaking; enumeration (viable count) using spread plate technique, and anti-microbial sensitivity testing, for lipophilic...
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Posted by admin on Jul 1, 2014 in |
The aim of the present study was to develop suitable controlled release injectable vancomycin thermoreversible chitosan/ β-glycerophosphate hydrogel system for treatment of orthopedic infections. The effect of different formulation variables on the gelation temperature was studied. The prepared vancomycin loaded Chitosan/β-glycerophosphate thermoreversible hydrogels were characterized regarding gelation temperature, in-vitro vancomycin release and enzymatic degradation rate in presence of lysozyme. Antimicrobial activity of the released vancomycin was tested against the gram-positive methicillin resistant Staphylococcus aureus (MRSA) by agar diffusion method. The results showed that chitosan/ β-glycerophosphate solution were able to form gels at body temperature depending on chitosan degree of deacetylation, concentration, pH and β-GP concentration. The prepared thermoreversible hydrogels sustained vancomycin release for 21 days. Released vancomycin concentrations ranged from 3 to 95 folds the reported minimal inhibition concentration (MIC 90) for MRSA at all release study time points. Chitosan/β-glycerophosphate thermoreversible hydrogels were enzymatically degradable and 17.57% of the gel weight remained after 21 days. The preserved antimicrobial activity found after release of the antibiotic from the hydrogel elected...
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Posted by admin on Jul 1, 2014 in |
Alizarin [1, 2 di hydroxy anthraquinone] is the major pigment, extracted from Rubia Cordifolia L. The aim of the present work is to extract manjith dye and alizarin pigment from Rubia Cordifolia L., and use it in the film coating of triphala guggle ayurvedic (TGA) tablet. The optimum extraction conditions for manjistha dye are 600C and 60 minute stirring with methanol (80%) and M:L ratio(1:10) with particle size 0.50mm. Separation and purification of alizarin was carried out by column chromatography. The purified alizarin was characterized by UV-Vis, and FT-IR, techniques. Acetylation of alizarin has been carried out with acetic acid in presence of sulphuric acid. Coating offers many benefits namely, improving asthetic qualities of dosage forms, masking unpleasant odour or taste, easy ingestion, and improving product stability. In the present study core tablets of triphla guggul are coated with manjistha extract, manjistha dye, pure alizarin, and acetylated alizarin. After coating, the stability of tablets were evaluated at regular intervals. The purpose of the stability testing is to provide proof...
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