Posted by admin on Sep 1, 2014 in |
In the present investigation, the bioefficacy of an indigenous plant was investigated with special reference to its antioxidant potential. Antioxidant effects of Methanolic, Chloroform and Ethyl acetate extracts of the leaves of Premna corymbosa Rottl. (family Verbenaceae) were tested based on DPPH (1, 1-diphenyl-2-picryl-hydrazyl), phosphomolybdenium assay, superoxide radical scavenging activity, and metal chelating activity. Results about the present investigation indicate that the methanol extract of the P. corymbosa showed strong antioxidant activities than the other solvent extracts. This study has made it clear that Premna corymbosa is an effective antioxidant. Such extracts could be more widely used in developing countries for the prevention and treatment of cancer. These extracts may be considered as good sources for drug...
Read More
Posted by admin on Sep 1, 2014 in |
The studies on the constituents of aerial parts of Stellaria media Linn. (Caryophyllaceae) have led to the isolation and characterization of three new compounds ─ (1), (2) and (3). Structure elucidation of the new compounds was carried out using 1H NMR, 13C NMR, Mass spectrometry together with other complementary techniques (UV and IR). The major findings of the study include: Compound 1 was separated as white amorphous solid from petroleum ether extract of S. media, Methanol extract of S. media was subjected to bioactivity-guided fractionation, and Compounds 2 and 3 were isolated, and characterized by different spectral studies. The interpretation of the three compounds include: compound 1 interpreted as 2, 4, 5, 7-tetramethyloctane, compound 2 as- 6-methylheptyl-3′-hydroxy-2′-methylpropanoate; and compound 3 as- 2, 2,...
Read More
Posted by admin on Sep 1, 2014 in |
Chlamydia trachomatis is the most common bacterial sexually transmitted disease. Over 100 million men and women worldwide are infected with Chlamydia at any point in time. The aim of this study to detect the presence of C. trachomatis antibodies and antigen among women on the reproductive age and to study the relationship between the presence of C. trachomatis and age, abortion and ectopic pregnancy. Five hundred women classify into three hundred pregnant women with a gestational age between 12 and 36 weeks and two hundred nonpregnant women. Two blood samples collected individually in two different containers, one EDTA container for PCR technique and plane container for ELISA testing during the period June 2011 to August 2013. Thirty-one samples (6.2%) were positive for C. trachomtis IgG only, while 8 samples (1.6%) were positive for both IgM and IgG. Out of 31 samples, 25 samples (5%) were positive by using the PCR technique. The results revealed statistically a significant relationship between PCR and previous abortion and ectopic pregnancy with P value,...
Read More
Posted by admin on Sep 1, 2014 in |
Dendrophthoe falcata L. is an important folk medicinal plant for its medicinal uses against different types of diseases. Therefore the present study was designed to investigate the antidiarrhoeal and insecticidal activities of aqueous, ethanol, chloroform and petroleum ether fractions of D. falcata leaves. Antidiarrhoeal activities of these extracts were evaluated in Swiss albino mice by three different approaches: (i) castor oil–induced diarrhea, (ii) castor oil–induced enteropooling and (iii) activated charcoal induced small intestinal transit. Three doses of the leaf extracts (50, 100 and 200 mg/kg.p.o.) and standard drug loperamide (5mg/kg.p.o.) were used to conduct the research which showed a significant (P<0.001) reduction in the severity and frequency of diarrhea (total number and weight of stools), the volume and weight of intestinal content, as well as intestinal transit compared to control group at dose-dependent manner. Ethanol extract exhibited maximum inhibition (83.47%) among the four fractions, which are slightly less than loperamide (89.23%) whereas aqueous fraction produced the lowest inhibition (50.95%). The insecticidal activity of these extracts was investigated by the...
Read More
Posted by admin on Sep 1, 2014 in |
The photostability of two fluoroquinolones: ciprofloxacin and lomefloxacin have been detected in tablets, and eye drops formulations using light-stability cabinet. Ciprofloxacin and lomefloxacin were subjected to stress conditions. The degradation products were well separated from the peak of the active substance. The stability of these compounds has been studied both in containers and under direct light in the light-stability cabinet. Samples were assayed immediately and at 1, 3, 6 months by stability-indicating high-performance liquid chromatography methods with a photodiode array detector. The determination was performed on C18 (250 × 4.6 mm, 5 µm). The first mobile phase consisted of 0.025M phosphoric acid, and acetonitrile (87:13) pumped at a flow rate 2ml/min for ciprofloxacin, while the second consisted of water, acetonitrile, triethylamine (80:20:0.3) pumped at a flow rate 1ml/min for lomefloxacin. The UV detector was operated at 278 nm for ciprofloxacin and 288 nm for lomefloxacin. The methods were suitably validated for linearity, accuracy, precision, robustness, and selectivity. All validation parameters were within the accep-tance range. Data analysis revealed that...
Read More
