Posted by admin on Oct 1, 2014 in |
Inosine Monophosphate Dehydrogenase (IMPDH) plays a very important role in Guanosine Monophosphate (GMP) biosynthesis. Type I hIMPDH is expressed at lower levels in all cells, whereas type II is especially observed in acute myelogenous leukemia, chronic myelogenous leukemia cancer cells, so it is thought to be an active target for leukemic drug design. MD-simulation studies of the solvated modeled structure of hIMPDH (PDB Id: 1B3O) in the presence of NAD+ have revealed some interesting feature on the role of some conserved water molecules in the binding of IMP to the enzyme. Based on H-bonding interaction of IMP with Asp 364, Arg 322, Asp 274, Cys 331 and Asn 303 residues in the X-ray and simulated structures, and the recognition dynamics of O2’ and O3’ ribose hydroxyl groups (of IMP) with the conserved water molecules, we have modified the hydroxyl group of IMP and modeled a few number of derivatives. Optimization of ligand structures, followed by docking in enzyme, solvation, energy minimization of the protein-ligand complexes and their successive all...
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Posted by admin on Oct 1, 2014 in |
Nasal infection or sinusitis is an inflammation of nasal passages caused by both viral and bacteriological pathogens. Antimicrobial resistance has universally recognized as growing problem concern about suitable therapy for nasal infection. The study was aimed at determining the prevalence and antimicrobial susceptibility against nasal infecting microorganisms. 50 clinical samples were taken from OPD of GMC Hospital, Bhopal (MP), India. Of the samples analyzed, 47 bacterial strains were isolated out of which 29 strains were of Gram positive bacteria (8 strains were of Staphylococcus aureus, 6 of Staphylococcus epidermidis, 7 of Streptococcus pneumoniae and 8 of Corynebacterium diptheriae) and 18 strains were of Gram negative bacteria (8 of Escherichia coli, 6 of Pseudomonas aeruginosa and 4 of Neisseria meningitidis). Antimicrobial susceptibility assay was performed by disc diffusion method according to the reference criteria of clinical and laboratory standard institute guidelines. In the present study antibiotic susceptibility pattern results showed maximum level of resistance in gram positive strains S. aureus 8 (100%), S. epidermidis 6 (100%) and C. diptheriae (8...
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Posted by admin on Oct 1, 2014 in |
The applications of the seed oil are defined by its quality through many factors, including seed origin. The study aims to investigate the physicochemical characteristics to compare the oil quality of plant Azadirachta indica (Family: Meliaceae), grown in different geographical areas, Malaysia, and Sudan. Oils were extracted through the Soxhlet method via hexane for the duration of 6 h. The characteristics of the oils from Malaysia (OMY) and Sudan (OSD) are; lipid content: 37.03 and 39.96%; physical state: liquid for both; color: greenish-brown and brownish-yellow; odor: peanut with garlic and only peanut; density: 0.95 and 1.06 g/cm3; refractive index: 70.90 and 69.80; pH: 4 and 5; moisture & volatile matter: 0.83 and 0.07%; acid value: 4.80 and 6.37 mg KOH/g; iodine value: 93.09 and 63.81 gI2/100g; peroxide value: 8.49 and 1.67 meq O2/kg; unsaponifiable matter: 1.84 and 0.91%; and free fatty acid: 4.75 and 3.21%. OMY and OSD recorded eight similar fatty acids: oleic- 20.46 and 52.02%, linoleic- 34.69 and 3.02%, stearic- 20.42 and 18.79%, palmitic- 18.66 and 20.79%,...
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Posted by admin on Oct 1, 2014 in |
About 80% of the population living in rural areas in developing countries depends on traditional medicine for their health care needs. Many of the powerful drugs used in modern medicines originated in plants. Today’s plant-based drugs treat a range of diseases, from headaches to cancer. The objective of the present study was to evaluate phytochemical, antioxidant potential, and cytotoxicity of methanolic extract of leaves of Trapa bispinosa Roxb. The phytochemical screening demonstrated the presence of carbohydrates, glycosides, alkaloid, saponins, and tannins. Total phenolic and flavonoid content of the Trapa bispinosa Roxb. extracts was determined by using the Folin- Ciocalteu reagent and aluminum chloride (AlCl3) method, respectively. Antioxidant activity was evaluated by using 1, 1-diphenyl-2-picryl-hydrazil (DPPH) free radical assay. In DPPH assay, free radical scavenging activity of the extracts was evaluated comparing with ascorbic acid at the wavelength of 517 nm and the IC50 value of Trapa bispinosa Roxb. extracts were found to be 1.58 µg/ml, whereas ascorbic acid showed the value of 1.129 µg/ml. Moreover, it showed moderate reducing...
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Posted by admin on Oct 1, 2014 in |
Siddha is one among the ancient systems of Indian medicine. Its reliability lies in the fact that it is time-tested, devoid of any drastic adverse effects and deep-rooted in the culture and heritage of India. The role of this system in treating chronic diseases is being widely felt nowadays. The challenges in treating such conditions with the currently available drugs (Non-Steroidal Anti-inflammatory Drugs – NSAID) often results in gastric irritation, renal damage, dependence, etc. Traditional Siddha medicines offer a wide range of Anti-inflammatory drugs which are polyherbal in nature and devoid of the above-said side-effects 1. This paper deals with Anti-inflammatory screening of such a medicine ‘Ashwathi chooranam’ documented in Classic Siddha text “Agathiar Vaithiya Kaaviyam” indicated for arthritis 2. Withania somnifera (Ashwagandha) is widely used in Siddha medicine. It is the main ingredient in many formulations prescribed for a variety of musculoskeletal conditions (e.g., arthritis, rheumatism), and as a general tonic to increase vitality, improve overall health and facilitates longevity 1. In our study, we reproduced an inflammatory...
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