Posted by admin on Jun 1, 2014 in |
Chicken anemia virus (CAV) encodes the protein VP3 also named “apoptin” that specifically induces apoptosis in human tumor cells. In this study, the VP3 gene cloned in p VAX1 plasmid vector was transfected in HeLa cells. The expression of CAV VP3 gene was detected using immunoperoxidase test and indirect fluorescent antibody technique. High level of gene expression was observed in HeLa cell culture. Successful expression of recombinant plasmid in mammalian cell lines shows that the plasmid could be further evaluated in field trials and hence useful tool for study of antitumor...
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Posted by admin on Jun 1, 2014 in |
Background: Neuropathic pain is a type of chronic pain that occurs when nerves in the central nervous system become injured or damaged. Currently only a limited number of topical treatments are available to treat it. Hence an attempt was made to alleviate symptoms of neuropathic pain by topical application of a local anaesthetic drug. Results: Microemulsions were formulated using Oleic acid as the oily phase and Tween 80 and ethanol in 1:1 ratio as the surfactant and cosurfactant respectively. The microemulsions thus formed were clear, transparent and aesthetic in appearance and showed low viscosity and near neutral pH. They exhibited electrical conductivity indicating they were o/w microemulsions. The particle size was in the submicron range while the polydispersity index was 0.4 indicating a uniform particle size distribution. A satisfactory and elegant gel was obtained using Xanthan gum as the gelling agent. In-vitro and ex-vivo drug diffusion indicated a sustained release of the drug over 8 hours. Anti-nociceptive activity was tested using hot plate and tail flick tests where the...
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Posted by admin on Jun 1, 2014 in |
The aim of this research work was to develop and characterise bilayer film and to investigate its potential as slow-release wound healing vehicle. The bilayer is composed of an upper layer impregnated with Diclofenac diethyl amine and a drug-free lower layer, which acted as a rate-controlling membrane. Solvent casting technique was employed to prepare transdermal films. Pre formulation studies are performed to determine solubility, melting point, compatibility and partition coefficient. Alginate based bilayer film for wound healing was successfully developed. The in vitro drug release study implied that by using the bilayer films which retain the drug for a longer period of time thus minimise the dressing changing frequency. The method adopted in this research work is genuine can be used as routine analysis of the...
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Posted by admin on Jun 1, 2014 in |
The present study is aimed to elucidate the antioxidant property and anti-inflammatory activity of hydro-ethanolic extract of Ganoderma lucidum (HEGL)known to possess medicinal activity against numerous diseases. The chemical composition of HEGL was quantified by colorimeteric technique in terms of total phenol and flavonoid content. Antioxidant activity was determined by 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The extract possesses strong anti-oxidant property in terms of high content of phenols, flavonoids and reducing power. Coumarin a known antioxidant and anticoagulant compound was identified and quantified by HPLC. Further, a dose dependent in vitro study for HEGL extract was carried out to observe anti-inflammatory activity in THP1 cells. HEGL extract significantly suppressed lipopolysacharide (LPS) – induced release of TNF-α, IFN-g and IL-1β in THP1 cells and significantly suppressed nitric oxide (NO) release in macrophages cells without causing any toxic effect. Additionally, HEGL extract also decreased NF-kB expression in LPS-treated cells indicating anti-inflammatory activity of hydro-ethanolic extract of Ganoderma lucidum (HEGL) at the...
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Posted by admin on Jun 1, 2014 in |
A new series of transition metal complexes of the type ML where M = Cu(II), Co(III) and Fe(III) and L = 2-benzoyl-3-(nitrophenyl)quinoxaline have been characterized and the structural features were arrived from the elemental analysis, magnetic susceptibility, molar conductance, FT- IR, UV-Vis and NMR spectral data. From the spectral measurements and magnetic susceptibility values a square planar geometry was proposed for Cu(II) complex and an octahedral geometry for Co(III) and Fe(III) complexes. The qualitative and quantitative antimicrobial activity test results proved that all the prepared complexes are very active especially against E. coli, S. aureus, C. albicans and A....
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