Posted by admin on Aug 1, 2014 in |
Biological/Toxicological activity of Cobra venom (CV) is usually estimated in terms of LD50 which varies at each time of test, when stored in liquid condition at non-native environment, as the CV contains most unstable complex proteins in it. Prepared & stored working solution of cobra venom at 5±3ºC in liquid condition for experimentation without any stabilizing agent, it becomes unstable and show huge variations in LD50 test result (performed on Swiss albino mice, weight: 17-22g). Such variations in biological activity of CV have adverse impact on venom dependent potency test of anti-snake venom sera (ASVS) which has direct commercial value. In this study, working stock solution of CV was prepared in the three different diluent sets (B, C and D) of solutions commonly used in laboratories and stored at 5±3ºC, which was tested for LD50 at pre-defined interval, up to 120 days. Obtain results were statistically calculated for variation from mean value in terms of Standard deviation (SD).The study result indicates that if the working stock solution of CV...
Read More
Posted by admin on Jul 31, 2014 in |
Background &Objective: To explore possible protective effect of neem leaf extract against methotrexate induced genotoxicity in the somatic cells (wing primordial) of Drosophila melanogaster. Methods: In the first phase of the experiment, neem leaf extract (NLE) at two doses was tested in the somatic cells (wing primordia) of Drosophila melanogaster (3rd instar trans heterozygous larvae resulting from cross of two bioactive strains) to probe effect on mutagenicity. In the second phase, effect of NLE at two doses (2.5% & 5%) was studied to probe its role in prevention of methotrexate induced genotoxicity. Concurrent negative control was run simultaneously in each experiment, where the larvae were exposed to the solvent (distilled water). The effect was observed and quantified under microscope as the number of spots (Clones) per wing. Results & interpretation: Only higher concentration of NLE (5%) showed significantly higher spontaneous mutation (2.7%) compared to untreated control group. Methotrexate induced 12.01 spots per wing (positive control). NLE at both doses (2.5 % & 5%) when administered concurrently with methotrexate, average...
Read More
Posted by admin on Jul 31, 2014 in |
In the present work six new benzoxazole derivatives were synthesized adopting the suitably selected scheme. The synthetic methodology included the synthesis of 2-(2-aminophenyl) benzoxazole (D1) by the reaction of anthranilic acid with 2-aminophenol in poly phosphoric acid. Substituted chloroacetanilides (A1-A6) were synthesized by the reaction of substituted anilines with chloroacetyl chloride. Finally compound 2-(2-(benzo[d]oxazol-2-yl) phenylamino)-N-(substituted phenyl) acetamide (2A-2F) were synthesized by the fusion of 2-(2-aminophenyl) benzoxazole (D1) and substituted chloroacetanilides (A1-A6). All the aynthesized compounds were subjected to antimicrobial screening against the bacterial strains i.e., Bacillus subtilis (MTCC-619), Streptococcus pneumonia (recultured), Escherichia coli (NCTC 6571) and Staphylococcus aureus (NCTC 7447). The compounds were also tested for antifungal potential against two fungal strains i.e. Aspergilus. Niger (NCIM NO. 618) and Candida albicans (NCYC 597). In all the testing a significant correlation existed between the antimicrobial potential and the concentration of test...
Read More
Posted by admin on Jul 31, 2014 in |
Objective: Study to elucidate the relation between depression, gender and blood groups. Methods: The study was prepared on 279 medical students of IMTU, who have spent more than four months in the medical school. A Performa for testing depression scale was given to the students. Blood groups and gender were analyzed. Results: It was evident that few blood groups like A and O shown significant prevalence of mild depression and group AB with severe depression. Females shown a high frequency of mild depression and males demonstrated an elevated severe depressed condition. Conclusion: The present study shows that blood groups and gender and depression scales are...
Read More
Posted by admin on Jul 31, 2014 in |
The term ‘transgenic plant’ is defined as the plants that are produced in the lab by artificial insertion of genes sometimes from the same species and sometimes entirely from the different kingdom. A major advantage of transgenic plant because of which it gains a lot of importance in today’s world is developing it for producing pharmaceutically useful compounds. Hence, they are acting as a natural bioreactor for producing therapeutic proteins and peptides in large amount. The present work deals with the investigation of producing a Human Neutrophil Peptide-1 (HNP-1) which is an antimicrobial peptide and commonly known as Defensin in Lycopersicum esculentum Mill. plant by Agrobacterium tumefaciens mediated genetic transformation technique. The cloning of HNP-1 was successfully done in a plant expression vector ‘pGreenI 0029 with 35S CaMV promoter’ by authors and described earlier. In this work we would like to describe transformation of pGreenI 0029 with HNP-1 gene and 35S CaMV promoter, inside Agrobacteium tumefaciens cells and then genetic transformation of Lycopersicum esculentum Mill.plant via recombinant Agrobacteium...
Read More
