Posted by admin on Jul 31, 2014 in |
The synthesis, characterization and spectroscopic studies of new N-substituted pthalimide analogues 2–7 with analgesic activity were described. The compounds were synthesized using phthalic anhydride with various appropriate amines (Triazolamine, Benzocaine, p-Nitro-aniline, pyrazinamide, Phenazone and glycine) in reflux synthesizer. The purity of the compounds was determined by TLC. With the physical and spectral data the structure of the new synthesized compounds were elucidated. The newly synthesized compounds were subjected for the screening of CNS activity by using standard experimental models. The analgesic activity of the selected compounds 2-7 were evaluated in vivo by ip carboxymethylcellulose (CMC) and acetic acid- induced ‘writhing’ test in mice. Compounds 2-7 were exhibited significant analgesic activity in hot-plate and acetic acid inducer screening test comparable to the control CMC (Carboxymethylcellulose) and...
Read More
Posted by admin on Jul 31, 2014 in |
The present study has been designed to investigate the effect of modulation of tumour necrosis α (TNF-α) on naloxone induced opioid withdrawal syndrome. Chronic morphine (5mg/kg,i.p) administration followed by a single injection of naloxone (8mg/kg, i.p) was used to precipitate opioid withdrawal syndrome in mice. Behavioral observations were made immediately after naloxone treatment. Withdrawal syndrome was quantitatively assessed in terms of withdrawal severity score and frequency of jumping, rearing, fore paw licking and circling. Administration of pirfenidone (200mg/kg, p.o) markedly and dose dependently attenuated naloxone induced morphine withdrawal syndrome. Thus, it is proposed that glial cells activation via toll like receptor (TLR) linked mechanism might be involved in the development of opioid dependence and precipitation of its withdrawal...
Read More
Posted by admin on Jul 31, 2014 in |
Turmeric, curcuma longa L of Zingiberaceae family is a widely cultivated spice in India and other Asian countries. Turmeric is rich in curcuminoids, and recognized for their broad spectrum of biological activities, curcuminoids vary in chemical structures, physico-chemical characteristics as well as the functional properties. This study focused on separation of individual curcuminoids from spent turmeric oleoresin considered as an industrial waste and its purification by column chromatography followed by purity analysis by HPLC. Chromatographic purification of spent turmeric oleoresin was done with Diaion HP 20 resin, fractions were collected. Curcuminoid enriched oleoresin fractions combined & then subjected to hexane precipitation, purified curcuminoid enriched fraction obtained. Purified curcuminoid enriched fraction subjected to silica gel column chromatography to separate the individual curcuminoids with chloroform: methanol at increasing polarity. 10ml each 40 fractions were collected and TLC profile done. Same Rf fractions collected & evaporated. Total curcuminoids of individual fractions were determined by HPLC. Recrystallization of each compound was done using methanol: chloroform (5:1) at 3°C. Curcumin, Demethoxy curcumin, Bisdemethoxy curcumin...
Read More
Posted by admin on Jul 31, 2014 in |
The cyclic albino rats of body weight group (120-130 gm.) were intravaginally treated with single dose (0.1 ml) neem oil at various durations to study its anti-fertility efficiency. It was observed that a significant increase in ovarian weight in weekly three months treated rat group to control and a significant decrease in follicle numbers in single ovary in weekly and fortnightly one and three months treated rat group in comparison to control. The disturbed reproductive cycle showed a highly significant increase estrus frequency from control in case of weekly and fortnightly one and three month treated rat group. But in rat group treated for monthly one and three months showed a significant increase. The reversibility showed a highly significant decrease in fortnightly one month in comparison to weekly one month treated rat group whereas monthly one month treatment showed a significant decrease from fortnightly one month treated group. The fortnightly three months treatment showed a significant increase in the reversibility with weekly and monthly three months treated group. All such...
Read More
Posted by admin on Jul 31, 2014 in |
Reactive oxygen species are associated with ageing process creating many health disorders such as Alzheimer, Parkinson, cancer, atherosclerosis and arthritis etc. These harmful actions of reactive oxygen species can be controlled to the some extent by using antioxidants which are present in plants, fruits, vegetables and spices. Turmeric, black pepper and clove are popular spices and have been used in traditional medicine. Their bioactive constituents i.e. curcumin, piperine and eugenol show many pharmacological impact on the nervous and neuromuscular system. Antioxidant activity of methanolic extract of above spices and their bioactive constituents have been evaluated synergistically by 1,1,-Diphenyl-2-picrylhydrazyl assay. Highest antioxidant potential is reported in mixture of curcumin and eugenol (97.38%) followed by methanolic extract of clove (95.91%) and mixture of methanolic extract of turmeric and clove (94.23%). Our studies clearly suggest that extract and their bioactive constituents individually does not shows as much as antioxidant potential as when they are mixed in definite proportion which may be due to synergistic...
Read More
