Posted by admin on Jun 1, 2014 in |
Complexes of Manganese(II) of general composition Mn(L)2X2 have been synthesized with ligand 2-heptanone semicarbazone and 2-heptanone thiosemicarbazone (where L = 2-heptanone semicarbazone or 2-hepatanone thiosemicarbazone and X=Cl–, SO42-, CH3COO–, NCS–, ClO4–) The IR spectral data of ligands indicate the involvement of sulphur/oxygen and nitrogen in coordination to the central metal. The molar conductance of the complexes of the complexes in fresh solution of DMSO lies in the range of 10 -20 ῼ-1 cm2mol-1 indicating their non-electrolyte behaviour thus the complexes may be formulated as [M(L2)X2].Ligands were characterized by Mass, NMR, and IR. All the complexes were characterized by elemental analysis, magnetic moments, IR, electronic and EPR spectral studies. All the manganese (II) complexes in present study show magnetic moment in the range of 5.90-6.05 B.M; corresponding to five unpaired electrons and hence these are high-spin complexes. The Schiff’s base ligands forms hexacoordinated complexes having octahedral geometry for...
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Posted by admin on Jun 1, 2014 in |
Aim and objective: To isolate the natural herbal binding agent from Eulophia ochreata Lindl. and study its evaluation properties using diclofenac as a model drug. Principle: Binders confer structural strength required by tablets during processing, handling, packaging and transportation. A number of plant mucilage, gums/hydrocolloids have been used as binding agents, suspending or emulsifying agents in both solid and liquid dosage formulation. They have been found useful in producing tablets with different mechanical strength and drug release properties for different pharmaceutical purposes. Mucilage is non-toxic and there wide availability has made them of continuing interest. Method: Present work reports extraction of mucilage of Eulophia ochreata Lindl. by multiple hot maceration technique using water and precipitation by alcohol (28% w/w yield). Physicochemical characteristics of mucilage, such as solubility, swelling index, loss on drying, pH and viscosity, were studied and also microbial load was determined. Mucilage was used in six different concentrations – 2, 4, 6, 8, 10 and 12% w/v. The granules were prepared by wet granulation technique and were...
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Posted by admin on Jun 1, 2014 in |
A study on the effect of methanol extract of red beetroot (MEBV) against CCl4 induced hepatotoxicity in male albino rats of Wistar strain (150 to 180g) was done. Five groups with six rats each were used as positive control, negative control and experimental with an oral dose of CCl4 throughintra-peritoneal (IP) route and MEBV at 100µgm/ml, 200µgm/ml & 300µgm/ml per os, respectively, for 14 days. After 14 days, animals were sacrificed; blood samples were obtained and analyzed. The results showed that CCl4 administration was associated with triple fold increases in the activities of alanine amino transferase (ALT), aspartate amino transferase (AST) and Bilirubin (P<0.05) compared with the respective mean control values. The enzymatic and non-enzymatic antioxidants such as, serum catalase, GSH, Vit. E, Vit.C, & SOD, were lower than the respective mean values of the normal control. The blood parameters have got reduced. The hapatochords and hepatocytes architecture was also observed to be disturbed. However, treatment with MEBV restored the enzyme activities of the liver ALT, AST, Bilirubin, WBC,...
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Posted by admin on Jun 1, 2014 in |
Antioxidants can protect the human body from free radicals and ROS effects. They retard the progress of many chronic diseases as well as lipid peroxidation. The commercially used synthetic antioxidants BHA and BHT have been suspected of being responsible for liver damage and carcinogenesis. Hence, a need for identifying alternative natural and safe sources of antioxidants has been created, and the search for natural antioxidants, especially of marine origin, has notably increased in recent years. The aim of this current study is to find the antioxidant effect of marine invertebrates of molluscan group comprising gastropods, bivalves and echinoderms methanolic extract using different in-vitro assays such as DPPH, reducing power and total antioxidant activity. The present findings imply that, bivalves possess higher antioxidant activity that formed as regular seafood for mankind than other groups investigated; hence, these methanolic extracts can be served as ironic agents of antioxidants and also used as pharmaceutical agents to prevent various degenerative diseases where it demands further research towards purification and mechanisms of...
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Posted by admin on Jun 1, 2014 in |
Chicken anemia virus (CAV) encodes the protein VP3 also named “apoptin” that specifically induces apoptosis in human tumor cells. In this study, the VP3 gene cloned in p VAX1 plasmid vector was transfected in HeLa cells. The expression of CAV VP3 gene was detected using immunoperoxidase test and indirect fluorescent antibody technique. High level of gene expression was observed in HeLa cell culture. Successful expression of recombinant plasmid in mammalian cell lines shows that the plasmid could be further evaluated in field trials and hence useful tool for study of antitumor...
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