Posted by admin on Jul 1, 2014 in |
Objective: To isolate and analyze the chemical components in crude methanolic extract from the South Indian medicinal plant of Kyllinga triceps by gas chromatography-mass spectrometry (GC-MS). Methods: Preliminary phytochemical screening of the extract was carried out according to the standard method described by Brindha et al. The shade dried powder was extracted with methanol by using soxhlet extractor. The GC-MS analysis of methanolic extract of Kyllinga tricepswas performed using a GC–MS equipment Thermo GC-TRACE ultra ver., 5.0, Thermo MS DSQ II. Experimental conditions of GC-MS system were as follows: TR 5-MS capillary standard non-polar column, dimension: 30 Mts, ID: 0.25 mm, Film: 0.25 µm was used and flow rate of mobile phase (carrier gas: He) was set at 1.0 ml/min. Crude samples which dissolved in methanol were run fully at a range of 50-650 m/z and the results were compared by using Wiley Spectral library search programme. Results: The phytochemical analysis of the extract revealed the presence of steroids, alkaloids, sugar, phenolic compounds, flavonoids, saponins, tannins and amino acids.The...
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Posted by admin on Jul 1, 2014 in |
Antifertility effect of ethanol extract of leaf of Eugenia singampattiana was observed in male albino rats. The relative weight of the testes and epididymis were decreased. The epididymal sperm count, motility and sperm abnormality were reduced significantly in treated rats. There was an increase in serum urea, creatinine and the activity of liver marker enzyme (SGOT) levels of drug treated rats. The results of the hormonal assay showed increased serum levels of FSH and estrogen but decreased levels of LH and testosterone when compared to control. The results of the present study concluded that, ethanol extract of leaf of E. singampattiana inhibited sperm concentration, motility and testosterone which might result in a male...
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Posted by admin on Jul 1, 2014 in |
The different extracts of leaf and bark of the plant Holoptelia integrifolia were investigated for in-vitro anti-inflammatory activity by human red blood cell membrane stabilization (HRBC) method. The HRBC membrane stabilization activity of the petroleum ether extract of leaf at the concentration of 200 mg/ml showed 68.12+/-1.05 % and petroleum ether extract of bark at the concentration of 200 mg/ml showed 71.25+/-1.72 %. Inhibition of denaturation in hypotonic solution with standard Diclofenac sodium 100 mg/ml showed 79.25%. Therefore, these extracts showed equipotent activity to Diclofenac...
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Posted by admin on Jul 1, 2014 in |
Acacia catechu is a multipurpose tree predominantly found in tropical and subtropical regions of India. The tree produces nitrogen rich fodder and green manure, high quality fuel wood and charcoal, strong durable poles and timber. The most useful products of this tree are Kutch or “Kattha” which is obtained from bark and heartwood of the tree. Commonly used plant part of Acacia catechu is bark which is a usual source of Kattha, is strong antioxidant, astringent, anti-inflammatory, antibacterial and antifungal in nature. The tree is also used as fodder for goat and cattle in many parts of India. However, the other plant parts of Acacia catechu especially from Central India have not been investigated for their nutritive value. The present study showcases a comprehensive investigation on different plant parts of Acacia catechu collected from Jabalpur. The results show that the young legumes of Acacia catechu can serve as a better source of nutrition for both human and...
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Posted by admin on Jul 1, 2014 in |
This communication reports on an easy and cost effective mycological medium, developed for primary isolation and culture of lipophilic yeasts. The medium has been modified and adjusted specifically to promote the growth of lipophilic yeasts and simultaneously suppress the growth of other fungi and bacteria. The traditional medium of Sabouraud’s dextrose agar (SDA) with olive oil overlay was modified by incorporating different oils (coconut, palm, corn, olive and sesame) in the medium. The oil incorporated SDA medium was compared with other routinely used media such as Modified Dixon’s, IMU – Mf and Modified Leeming – Notman, for the culture of lipophilic yeasts. From the results obtained, it was observed that the medium with sesame oil exhibited the highest viable count within 48 hours. Thus, this medium can be used in the conventional microbial procedures such as isolation of pure culture by streaking; enumeration (viable count) using spread plate technique, and anti-microbial sensitivity testing, for lipophilic...
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