Posted by admin on Jul 1, 2014 in |
Biofilm are a matrix of microorganisms which are adhered to and colonized a surface. When formed they are very difficult to remove and act as a source of contamination in processing environments. As bacteria in biofilm exhibit enhanced resistance to antibiotics and clearance by the host immune system, the resistance of enteropathogenic bacteria to commonly prescribed antibiotics is increasing both in developing as well as in developed countries. Resistances have emerged even to newer, more potent antimicrobial agents. This study was under taken to investigate the presence of multidrug resistance producing biofilm forming Aeromonas hydrophila in human clinical samples. A total of 150 urine samples were collected from private hospital in Tiruchengode during the period of six month. Among these only 75 isolates were found to be positive for Aeromonas hydrophila. The Starch-Ampicillin agar were used as a selective presumptive isolation medium for the isolation of bacterial isolates and confirmed as Aeromonas hydrophila were determined by using standard biochemical analysis according to Bergey’s manual of systematic Bacteriology. Slime producing...
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Posted by admin on Jul 1, 2014 in |
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantitation of Moxifloxacin Hydrochloride (MOX), Ketorolac Tromethamine (KET) in pharmaceutical dosage form. Chromatographic separation was carried out in a Zorbax eclipse plus, C18 column (250 mm × 4.6 mm; 5 µm particle size) with simple mobile phase composition of 10 mM Potassium dihydrogen phosphate buffer with Triethylamine (pH 3.14) and acetonitrile (40:60, v/v) at a flow rate of 0.5 mL min-1 where detector was set at 302 nm with a total run time of 8 mins. The method was linear over the concentration range of 40-100, µg mL-1 with a correlation coefficient of 0.9891 and 0.994. Limit of quantifications (LOQ) of 13.3, 26.3 and limit of detections (LOD) 4.4, 8.7 µg mL-1 for MOX, and KET respectively. Accuracy and precision values of both within-run and between-run obtained from six different sets of three quality control (QC) samples analyzed in separate occasions for both the analytes...
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Posted by admin on Jul 1, 2014 in |
Non-nucleoside reverse transcriptase inhibitors (NNRTI) are a group of small hydrophobic compounds with diverse structure that specifically inhibit HIV-1 reverse transcriptase (RT) by allosteric bind to change its conformation. NNRTIs interact with HIV-1 RT by binding to a single site on the p66 subunit of the p66/p51 heterodimeric enzyme, termed the NNRTI-binding pocket (NNRTI-BP), binding interaction results in both short-range and long-range distortions of RT structure. In this article, we chose T-70(Rilipivirine) as a base structure for virtually identification of more/similar efficient drug like leads then T-70 using five different PDB structures (4KFB, 4IG3, 4IF3, 4GIQ, 3BGR) of RT fromPDB database ‘RCSB’ versus chemical compounds database ‘ZINC’ using Schrodinger and Discovery Studio software. Using molecular constraint search with similarity coefficient ‘Tanimoto’, 67500 ligands were extracted and docking analysis resulted in few better efficient in docking properties and in other computational medicinal parameters have reported, and they may further undergo through high end extensive virtual investigation and...
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Posted by admin on Jul 1, 2014 in |
Cordia dichotoma L. family Boraginaceae is a medium sized tree, is taken as food. The immature fruits are pickled and are also used as vegetable. The various extracts and fractions of Cordia dichotoma were screened for their free radical scavenging properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and comparing it with butylated hydroxyl toluene (BHT) as standard antioxidant. The methanolic extract of seeds of Cordia dichotoma Linn and its fraction containing Taxifolin showed promising (P<0.001) DPPH free radical scavenging activity at a concentration of 100...
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Posted by admin on Jul 1, 2014 in |
The role of liquid membrane hypothesis has been studied in the mechanism of action of Ertapenem. In the present study transport of selected permeants (glucose, p-amino benzoic acid and ions like magnesium, ammonium, phosphate, calcium, sodium, potassium and chloride) through liquid membrane generated by Ertapenem in series with supporting membrane has been studied. The results indicate that the liquid membrane generated by Ertapenem inhibit the transport of various essential bio-molecules and permeants into the cell. The data indicate that modification in permeability of different permeants in the presence of the liquid membrane is likely to play significant role in the biological actions of Ertapenem. It seems that the formation of liquid membrane by Ertapenem may also contribute for the bactericidal activity of the drug, in addition to its conventionally established mechanism i.e. inhibition of cell wall...
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