Posted by admin on Jun 1, 2014 in |
This research manuscript describes simple, sensitive, accurate, precise and repeatable RP- UPLC method for the simultaneous determination of Ceftriaxone (CEF) and Tazobactam (TAZ) Injection in combine dosage form. The sample was analyzed by reverse phase C18 column (Acquity UPLC BEH 100 × 2.1 mm ID, 1.7 µm) with mobile phase. In mobile phase, Solution A containing Potassium Dihydrogen Phosphate buffer (pH adjusted to 6.5±0.2 with Orthophosphoric acid), Citric acid buffer (pH adjusted to 5.0±0.2 with NaOH solution) and Acetonitrile and Solution B containing Tetradecyl ammonium bromide, Tetraheptyl ammonium bromide and Acetonitrile in the flow rate of 0.3 ml/min. Quantification was achieved 230 nm with PDA detector. The retention time for Ceftriaxone and Tazobactam was found to be 2.83 and 1.72 minute respectively. The linearity for Ceftriaxone and Tazobactam was obtained in the concentration range of 40-280 µg/ml and 5-35 µg/ml respectively. Ceftriaxone and Tazobactam API and market formulation were subjected to acid and alkali hydrolysis, oxidation, thermal and photolytic forced degradation. The peak purity of drug substance and drug...
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Posted by admin on Jun 1, 2014 in |
A rapid and simple spectrophotometric method is reported here for the determination of paracetamol in a commercially available tablet formulation. The method is based on the diazotization of hydrolyzed paracetamol with 8-hydroxyquinoline as a coupler to form stable azo dyes color solution. The concentration of drug paracetamol was investigated by spectrophotometrically. The azo dyes formed with 8-hydroxyquinoline as coupling agents follow the Lambert-Beer’s law in the range of 2 to 10 μgmL-1 of paracetamol. Sandells sensitivity and molar absorptivity observed for azo dye coupled with 8-hydroxyquinoline was found 7.9 µg mL-1cmand 1.9 ×104 Lmol-1cm-1 respectively. The percentage recovery of the drug was found in the range of 97.4 to 100.2 % the coupling agent 8-hydroxyquinoline. The data obtained using 8-hydroxyquinoline as a coupler were compared with the data obtained with 2-naphthol as a coupler suggests that dye formed with 8-hydroxyquinoline is more stable than the 2-naphthol. The method reported here may be used to determine the trace amount of paracetamol in any clinical samples with accuracy and...
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Posted by admin on Jun 1, 2014 in |
Objective: To study detailed Pharmacognosy of the root of Operculina turpethum (Convolvulaceae) well known Ayurvedic drug (trivrit). Method: Macroscopy, microscopy, physicochemical analysis, preliminary phytochemical testing of the root and other WHO recommended methods for the standardization were done. Results: The periderm cells are tabular in shape; the phloem cells are homogeneous, tabular in shape and have suberized walls. The phelloderm cells are also tabular in shape and have cellulose walls. Irregular masses of squarish sclerenchyma cells are seen inner to the periderm and with outer cortical zone. Sclereids are also seen in small masses in the interior portion of the cortex. The cortical cells are densely loaded with starch grains and sparsely distributed calcium oxalate crystals. Secondary xylem is the thick and dense central cylinder comprising wide, circular thin walled vessels and thick walled lignified fibers. The vessels are solitary and diffuse in distribution. The xylem parenchyma cells are thick, tangential layered and they are apotracheal layered type. The parenchyma layers are away from the...
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Posted by admin on Jun 1, 2014 in |
A direct chiral separation method development was carried out for sitagliptin and its (S)-enantiomer on ten diverse chiral stationary phases. Chiral stationary phases, which were tested include, R, R Whelk-O1 column, macrocyclic glycopeptides namely, Chirobiotic R (Ristocetin A), Chirobiotic V (Vancomycin), Chirobiotic T (Teicoplanin), Chirobiotic TAG (Teicoplanin aglycone), OJ-H, OJ-RH belonging to tris (4-methylbenzoate) of cellulose. Chiral selectivity was observed on polysaccharide based CSP columns namely Chiralpak IA-3, Chiralpak IC-3 belonging to tris-(3,5-dimethylphenyl carbamate) of amylose, tris-(3,5-dichlorophenyl carbamate) of cellulose respectively and OD-H (tris-3,5-dimethylphenylcarbamate) of cellulose. Better enantioselective separation has been achieved on cellulose tris-(3,5-dichlorophenyl carbamate) column (Chiralpak IC-3), using IPA and n-hexane as mobile phase, both containing 0.05% ethylene diamine and at 0.5 mL/min flow rate. Detection was carried out at 266nm using PDA detector and column maintained at 35ºC. The method was validated for precision, accuracy, linearity and robustness. The advantages of the method are rapid equilibration and less solvent consumption due to short column length. Efficient enantio separation (Resolution 3.38) is due to small particle size...
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Posted by admin on Jun 1, 2014 in |
In present study the phytochemical analysis of the plant Acacia nilotica was done using GCMS method and seven compounds were identified as 3-picoline-2-nitro, 1-acetyl beta carboline, Hydroxy citronellal, Trans decalone, Propionic acid-2-chloro, ethyl ester, Lavandulyl acetate and D-Glucoronic acid. The anticancer activity of these seven compounds were analyzed with molecular docking study against the protein S-adenosyl-L-methionine decarboxylase which is found elevated in person with liver cancer. Among the seven phytochemicals identified, hydroxy citronellal followed by 1-Acetyl ß carboline was observed to have maximum score with S-adenosyl-L-methionine decarboxylase in molecular docking study, Glucoronic acid scores maximum in number of Hydrogen bonds. And taking into consideration all the scoring parameters, Glucoronic acid is considered as the best fit. Further studies are needed for the isolation of this particular phytochemical and subject it to further cell line testing and animal testing for anticancer activity and can be processed to be an effective and cheaper drug due to higher biomass availability of Acacia nilotica...
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