Posted by admin on Apr 1, 2014 in |
Onion, garlic and fenugreek are widely cultivated in Egypt and many reports confirmed their antidiabetic activities as used separately. Current study focused on these three herbs when present together in a mixture. All dried herbs were finely powdered to obtain homogenous mixtures. Pre-formulation studies were conducted on each individual herb including determination of foreign matter, loss on drying, acid insoluble ash and water contents. Analysis of active components responsible for the antidiabetic activity was also carried out using GLC and HPLC techniques. Pharmaceutical capsules were prepared by blending garlic, onion, and fenugreek seed powders in a ratio 1:1:2 respectively. The blend was granulated using 2% alcoholic solution of PVP k-30. The Disintegration testing of hard gelatin capsules was performed according to the USP, the capsules were disintegrated completely within 15 minutes in both distilled water and acetate buffer (0.05 M). In vitro release studies showed variable dependence of the active components on the pH of the release media. Pharmacological studies conducted on hyperglycemic rats indicated that both onion- and...
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Posted by admin on Apr 1, 2014 in |
A new simple, rapid, accurate, precise and sensitive RP-HPLC method was developed for the estimation of sildenafil citrate in bulk and pharmaceutical dosage form. The separation of sildenafil citrate was achieved on a Phenomenex C18 Column (150 X 4.6 mm i.d, 5µ particle size) with a mobile phase consisting of methanol and Buffer (50:50 % v/v). The flow rate was 1.5 ml/min and photodiode array detection at 290nm. The retention time of Sildenafil citrate was found to be 4.842 min. Calibration curve was found to be linear in the concentration range of 70 – 210 µg/ml and the correlation coefficient value was found to be (R2) 0.9998. LOD and LOQ were 2.361ng/ml and 7.156ng/ml respectively. The RSD value of Precision and recovery studies were less than 2 %, indicated that the method was precise and...
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Posted by admin on Apr 1, 2014 in |
This work has been undertaken to study the antimicrobial efficiency of Hexane, Ethyl acetate, Ethanolic extracts of herbal plants (Anthocephalus cadamba, Allium sativum, Origanum vulgare, Ocimum sanctum) against human pathogens like Staphylococcus aureus (MTCC-3160), Escherichia coli (MTCC-1652) and fungi Aspergillus niger (MTCC-282) by using agar well diffusion method. All the plants showed significant activity against all pathogens, but the alcoholic extract of Ocimum sanctum showed maximum zone of inhibition and minimum inhibitory concentration against all the pathogens. The minimum zone of inhibition and comparatively greater inhibitory concentration were determined in hexane and ethanolic extract. The Spectrum activity of alcoholic extracts of these plants could be a possible source to obtain new and effective herbal medicines to treat various infectious...
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Posted by admin on Apr 1, 2014 in |
Tridax procumbens (Linn.) is an important medicinal plant belonging to the family Asteraceae. It is known for a number of pharmacological activities like antidiabetic, anticancer, anti-inflammatory, wound healing, hepatoprotective and antioxidant activity.Flavonoid “Quercetin” was isolated from the flowers of this plant. Dried flowers were Soxhlet with petroleum ether, chloroform, and methanol successively. The methanolic fraction so obtained was successively extracted with petroleum ether, diethyl ether and ethyl acetate. The ethyl acetate fraction was hydrolyzed with 7% H2SO4 and extracted with ethyl acetate to obtain crude Quercetin. The crude product was recrystallized with dilute ethanol to get pure Quercetin. The purified material was subjected to various chromatographic and spectral techniques such as UV, IR, HPTLC, HPLC, NMR etc. and was identified as “Quercetin”. This study is also of practical importance because quercetin is an important constituent of Tridax procumbens and it has many uses such as in cancer, diabetes, inflammation, & in viral infections...
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Posted by admin on Apr 1, 2014 in |
The main objective of the present study is to enhance the dissolution of clopidogrel bisulphate in intestinal fluids by formulating into mini-tablets with in-situ acidifying agent mini-tablets. The solubility data reveals, Clopidogrel bisulphate is freely soluble in pH 2.0 and the solubility decreases with increase in pH. Whereas, with weak acid, the solubility of clopidogrel is increased at pH 5.0 blank fed state simulated intestinal fluid and & pH 6.5 blank fasted state simulated intestinal fluid. Hence, tartaric acid is selected in the core tablet to enhance the dissolution of clopidogrel tablets at intestinal fluid pH 5.0 & pH 6.5. Formulation was evaluated with different levels of tartaric acid in core tablet. Formulation with 50mg/unit of tartaric acid showed the extent of dissolution of 100% in pH 5.0 blank fed state simulated intestinal fluid and 96% in pH 6.5 blank fasted state simulated intestinal fluid. To control the impact of humidity on degradation, a moisture protective layer is coated on core tablet with non-aqueous solvent, using conventional coating pan....
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