Posted by admin on Jun 1, 2014 in |
In the present study, metronidazole was used for preparing floating dosage forms that are designed to retain in the stomach for a long time and have developed as a drug delivery system for better eradication of Helicobacter Pylori in peptic ulcer diseases. Hydroxypropyl methylcellulose (HPMC K4M) and Carbopol-934P of different ratio were used to prepare Hydrodynamically Balanced Systems (HBS) by direct compression technique. The prepared HBS tablets were evaluated in terms of their pre-compression parameters and post-compression parameters like hardness, friability, weight variation, uniformity of drug content, percentage swelling, in-vitro floating studies, in-vitro drug release and short term stability studies. The floating properties and drug release characteristics were determined for the prepared HBS in 0.1 N HCl dissolution media. All the HBS formulations showed good in-vitro floating properties with an optimum concentration of gas generating agents; sodium bicarbonate and citric acid. The rate of drug release decreased with increased hardness of tablets. Optimum concentration of HPMC K4M (140 mg) and Carbopol-934P (10 mg) along with excipients was found to...
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Posted by admin on Jun 1, 2014 in |
Evaluation of the anticancer effect of Trapa acornis (Family- Trapaceae) which has long been prescribed for various infectious and malignant diseases. The shells extract of Trapa acornis has been carried out by following due procedure. Cell lines, cultures were incorporated and procured for in-vitro and in-vivo study with reference to cell proliferation assay, cell viability assay and cell cycle analysis. Bio-assays of extracts from Trapa acornis showed that a fraction (fraction 3) from an ethanolic extract had an anticancer effect on SKBR3 and MDA-MB435 human breast cancer cells. In MDA-MB435 cells, cell cycle analysis showed that the plant extract fraction 3 induced the accumulation of cells in G2/M phase, whereas no significant change in cell cycle was detected in SKBR3 cells. The results indicated that the extract fraction could induce cell cycle arrest in some way. However, further investigation is needed to assess the molecular mechanisms mediated anticancer activities of this...
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Posted by admin on Jun 1, 2014 in |
Three Simple, rapid, sensitive, accurate and precise stability-indicating spectrophotometric methods was developed for the determination of cefdinir in bulk powder and in pharmaceutical preparation. Method (A) Ratio derivative method(1DD); is used for the determination of intact cefdinir in presence of its degradation product at 307 nm in the range of 2 – 14 µg ml-1 with LOD of 0.049 µg ml-1 and LOQ of 0.162 µg ml-1. Method (B) Ratio difference method ;is based on measuring the difference in the amplitude of intact cefdinir in presence of its degradation product at two different wavelengths ,this is done at 219 nm and 250 nm in the range of 2 – 14 µg ml-1 with LOD of 0.050 µg ml-1 and LOQ of 0.165 µg ml-1. Method (C) First derivative method(1D); is used for the determination of intact cefdinir in presence of its degradation product at 304 nm in the range of 2 – 14 µg ml-1 with LOD of 0.034 µg ml-1 and LOQ of 0.114 µg ml-1. The methods...
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Posted by admin on Jun 1, 2014 in |
The objective of this study was to characterize the non-enzymatic and enzymatic antioxidants and Scavenging activities of different extracts (Petroleum ether, Benzene, Chloroform, Acetone, Ethanol, Methanol and Water) of dried flowers powder of Blepharis molluginifolia (Acanthaceae). Enzymatic antioxidant activity such as Superoxide Dismutase, Glutathione –S-transferase, peroxidase and Catalase of B. molluginifolia flowers were high in methanol extract. Non enzymatic antioxidants like Tocopherol, Ascorbic acid, Phenols, Carotenoids and Lycopene were analysed. Acetone extract showed highest Total Phenol content and Vitamin-C were 100.66±1.5 mg GAE/gm & 52.08±2.42 mg/gm respectively. Scavenging activities like DPPH, ABTS and H2O2 were high in Methanol extract. In this study, the non-enzymatic antioxidant activity was found to be significant, which prove to be a better scavenger of free radical in comparison to enzymatic extracts in the shade dried...
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Posted by admin on Jun 1, 2014 in |
The present study investigates neuroprotective effect of hydroxy citric acid (50%) on phenytoin induced cognitive impairment and oxidative stress in rats.Male Wistar rats were divided into 6 groups and each group received vehicle, phenytoin (25mg/kg, i.p) and hydroxy citric acid (400 and 800mg/kg, orally) for 14 days respectively. Memory and learning was evaluated by using the Rectangular maze, Morris water maze and Locomotor activity were evaluated. On day 14, animals were sacrificed, brain tissue was dissected. The Acetylcholine Esterase (AChE) levels, antioxidants levels were estimated in the brain homogenate.The histopathological studies were done to check for the gross changes in histology. Phenytoin (25mg/kg, i.p) produced significant deficits in learning and memory as indicated by significant increase transfer latency in rectangular maze, Morris water maze and decrease number of counts in Actophotometer. It also produced significant elevation in brain AChE, MDA levels along with reduction in Catalase and DPPH levels. Hyrdroxy citric acid (400 and 800mg/kg) orally, when co-administered with phenytoin, significantly prevented phenytoin induced cognitive impairment, oxidative stress and...
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