Posted by admin on Jun 1, 2014 in |
Schistosomiasis is a major communicable disease with a great impact on both public health and socioeconomic status of the developing world.It is highly prevalent in tropics and causes morbidity and mortality in developing countries including Sudan. Because of its chronicity and robust immunomodulatory activity, the effects of Schistosomiasis on other diseases such as allergies, autoimmunity and infectious diseases have been studied extensively in both epidemiological and experimental settings. Salmonella spp. is a common water- and food-borne cause of gastrointestinal and systemic diseases worldwide.Approximately, 2 million individuals die each year of diarrhea and Salmonella is a leading cause of this illness. A relationship between persistent as well as recurrent Salmonella bacteremia and schistosomiasis has been described both in adults and children. The present cross-sectional study was conducted to determine the relationship between schistosomiasis and salmonellosis in Helat Mahajoub in AL-Gezira Agricultural Scheme. Urine and faecal samples were collected from 288 individuals and investigated for Schistosoma ova using the modified Kato thick smear technique and the concentration technique. 207 subjects were...
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Posted by admin on Jun 1, 2014 in |
Ocimum basilicum L. and Ocimum gratissimum L. (Lamiaceae) are widely distributed aromatic herbs used in ethnomedicinal management of a range of inflammatory disorders. In the present work we evaluated the topical anti-inflammatory effects of the volatile constituents extracted from the fresh leaves of these plant species. Fresh leaves of the plants were subjected to hydro distillation to obtain the volatile oils OBV and OGV from O. basilicum and O. gratissimum respectively. The fresh leaves were also extracted with n-hexane to obtain OBHE and OGHE respectively. OBV, OGV, OBHE and OGHE were screened for anti-inflammatory effect using xylene-induced ear edema as a model of inflammation. Their chemical constituents were also analysed using GC/MS apparatus. At 50 µg/ear OBV, OGV, OBHE and OGHE exhibited significant (P<0.05) topical anti-inflammatory effect with edema inhibitions of 50.0, 63.3, 62.7 and 80 % respectively. The effects were comparable (P<0.05) with that of 100 µg/ear hydrocortisone (% edema inhibition of 54.8). 11 of the compounds from OGV are monoterpenes while 4 are sesquiterpenes. 8 of the...
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Posted by admin on Jun 1, 2014 in |
Bryophyllum pinnatum is widely used in ayurvedic system of medicine as astringent, analgesic, carminative and also useful in diarrhoea and vomiting. It is naturalized throughout the hot and moist parts of India. The leaves of B. pinnatum have a variety of uses in the traditional system of medicine in India. They are eaten for diabetes, diuresis, dissolving kidney stones, respiratory tract infections, as well as applied to wounds, boils, and insect bites. It is useful for preventing alcoholic, viral and toxic liver damages. The aqueous extract of this plant have shown anti-inflammatory, anti-diabetic, anti-tumor and cutaneous leishmanicidal activities. Maximum percent shoot and root regeneration took place in A3 and A16 set respectively which was 80±1.45 % for the former and 75±1.84 % for the latter. The shoot regeneration which was acquired in set A3 was statistically highly significant while regeneration in A15 was more and statistically highly...
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Posted by admin on Jun 1, 2014 in |
This research manuscript describes simple, sensitive, accurate, precise and repeatable RP- UPLC method for the simultaneous determination of Ceftriaxone (CEF) and Tazobactam (TAZ) Injection in combine dosage form. The sample was analyzed by reverse phase C18 column (Acquity UPLC BEH 100 × 2.1 mm ID, 1.7 µm) with mobile phase. In mobile phase, Solution A containing Potassium Dihydrogen Phosphate buffer (pH adjusted to 6.5±0.2 with Orthophosphoric acid), Citric acid buffer (pH adjusted to 5.0±0.2 with NaOH solution) and Acetonitrile and Solution B containing Tetradecyl ammonium bromide, Tetraheptyl ammonium bromide and Acetonitrile in the flow rate of 0.3 ml/min. Quantification was achieved 230 nm with PDA detector. The retention time for Ceftriaxone and Tazobactam was found to be 2.83 and 1.72 minute respectively. The linearity for Ceftriaxone and Tazobactam was obtained in the concentration range of 40-280 µg/ml and 5-35 µg/ml respectively. Ceftriaxone and Tazobactam API and market formulation were subjected to acid and alkali hydrolysis, oxidation, thermal and photolytic forced degradation. The peak purity of drug substance and drug...
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Posted by admin on Jun 1, 2014 in |
A rapid and simple spectrophotometric method is reported here for the determination of paracetamol in a commercially available tablet formulation. The method is based on the diazotization of hydrolyzed paracetamol with 8-hydroxyquinoline as a coupler to form stable azo dyes color solution. The concentration of drug paracetamol was investigated by spectrophotometrically. The azo dyes formed with 8-hydroxyquinoline as coupling agents follow the Lambert-Beer’s law in the range of 2 to 10 μgmL-1 of paracetamol. Sandells sensitivity and molar absorptivity observed for azo dye coupled with 8-hydroxyquinoline was found 7.9 µg mL-1cmand 1.9 ×104 Lmol-1cm-1 respectively. The percentage recovery of the drug was found in the range of 97.4 to 100.2 % the coupling agent 8-hydroxyquinoline. The data obtained using 8-hydroxyquinoline as a coupler were compared with the data obtained with 2-naphthol as a coupler suggests that dye formed with 8-hydroxyquinoline is more stable than the 2-naphthol. The method reported here may be used to determine the trace amount of paracetamol in any clinical samples with accuracy and...
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