Posted by admin on Jul 1, 2014 in |
Background: Familial Hypercholesterolemia (FH) is an autosomal dominant disorder caused by mutation in the LDL receptor gene and characterized by raised LDL-C, tendon xanthomata and premature atherosclerosis. Existing therapies for FH such as statins and LDL apheresis do not offer adequate lipid control in most patients, which has led to the search for more definite alternatives. Two new drugs have been approved by the US FDA in the recent past for FH, namely mipomersen, an anti-sense oligo-nucleotide and lomitapide, a microsomal triglyceride transfer protein. Methods: We did a literature search across PubMed to retrieve articles related to efficacy and safety of mipomersen and lomitapide. Results:Mipomersen has been found to have reasonable efficacy in clinical trials over and above that seen with concomitant statin therapy in FH. Lomitapide has also shown evidence of adequate LDL-C reduction in clinical trials though the number of studies performed with lomitapide is relatively fewer. The subcutaneous route of administration for mipomersen may affect compliance especially with long term treatment. The most common adverse reactions...
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Posted by admin on Jul 1, 2014 in |
Simple, accurate and precise spectrophotometric methods for quantitative determination of four drugs viz., Alfuzosin (ALF), Esomeprazole (ESO), Indomethacin (IND), Ketorolac Tromethamine (KET) have been developed based on oxidation of the drugs by alk.KMnO4. Kinetics of the oxidation reaction is followed spectrophotometrically, as one of the reaction product, Mn(VI), absorbed at 610 nm. Initial rate and fixed time method are used for the construction of calibration curves Beer’s law is obeyed in the range 12.5-100µg mL-1 for ALF; 25-150 µg mL-1 for ESO; 2.5-15 µg mL-1 for IND and 6.25-37.5 µg mL-1 for KET. Recovery studies using pure samples and pharmulations in the Beer’s Law limits have been carried out and the methods have been validated in terms of ICH guidelines. Statistical analysis in terms of student’s t- test and variance F- test demonstrate high accuracy and precision and suggest the methods can be applied in bulk drug and pharmaceutical industries.Simple, accurate and precise spectrophotometric methods for quantitative determination of four drugs viz., Alfuzosin (ALF), Esomeprazole (ESO), Indomethacin (IND), Ketorolac...
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Posted by admin on Jul 1, 2014 in |
The evolution of green chemistry in the production of nanoparticles has wrapped up an immense consideration because traces of chemicals left unreacted in the chemical synthesis process can be precarious. Owing to copious interest a competent protocol for the production of ZnO NPs without calcinations was developed by green synthesis method using aqueous leaf extracts of Azadirachta Indica and Emblica Officinalis. The aqueous leaf extract acts as a solvent with manifold roles as promoter, stabilizer and template for the synthesis of nanoparticle. The formed nanoparticles were characterized by SEM, XRD, FTIR, and EDAX for its morphology, size, crystallinity and percentage composition. The results confirmed the formation of nanoflowers of 51 nm for Azadirachta Indica and nano flakes of 16 nm for Emblica Officinalis. Though both the green synthesis is trendier for nanoparticle synthesis, the biogenic green fabrication of Azadirachta is better due to its morphology, particle size and crystallinity. Green synthesis using Azadirachta is found to be the best stabilizer for synthesizing nanoparticle. And the study was further initiated...
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Posted by admin on Jul 1, 2014 in |
The World Health Organization (WHO) defines traditional medicine as “the health practices, approaches, knowledge and beliefs incorporating plants, animals and mineral-based medicines, spiritual therapies, manual techniques and exercises, applied singularly or in combination to treat, diagnose and prevent illness or maintain wellbeing. Various compounds have been isolated from Bridelia micrantha some of which areFriedelin, Taraxeronie, Epifiedelinol, Taraxerol, Garlic acid, Ellagic acid and sitosterol. Fresh stems of B. micrantha collected, dried, pulverized and mixed with sterile distilled water, extract left for 72hrs and stirred at 3hourly, filtered and concentrated in a water bath, stored in a refrigerator. Experiments were carried out using mice and wister rats of both sexes. The LD50 following the intraperitoneal route was estimated to be 20.3 mg/kg. The surviving animals following intraperitoneal route exhibited toxicological signs of writhing, decrease loco-motor activity, sedation and dose dependant mortality. Haematological parameters in the rat after 28days of daily oral administration show no significant difference between the treated groups and control. However, lymphocytes at the dose of 1000mg/kg indicate significant...
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Posted by admin on Jul 1, 2014 in |
A simple, precise, accurate, economical and reproducible HPLC method for estimation of Olmesartan in tablet dosage form has been developed. Quantitative HPLC was performed with HITACHI L2130 with D Elite 2000 Software with Isocratic with UV-Visible Detector (L-2400), PUMP (LC-IOAT). C18 Develosil ODS HG-5 RP 150mm x 4.6mm 5µm particle sizecolumn receptor in vascular muscle. Its action is therefore independent of the pathway of angiotensin II synthesis was used in the study.The mobile phase of ACN: phosphate buffer (pH 3.1) was used in this study. The conditions optimized were: flow rate (1.0 ml/minute), wavelength (205nm) and run time was 10 min; column temperature was maintained at 400C. Retention time was found to be 6.31 min. The linearity was found to be in the concentration range of 10-100 mg/ml. The developed method was evaluated in the assay of commercially available tablets OLMESARcontaining Olmesartan. The amount of drug in tablet was found to be 40mg. Results of analysis were validated statistically and by recovery studies. The recovery studies 99.27 % was...
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