Posted by admin on Feb 1, 2014 in |
Objective: To study the leaves of Simarouba glauca for their antibacterial, antioxidant, haemolytic, thrombolytic activities and to perform phytochemical evaluation. Methods: The three extracts (chloroform, methanol, ethyl acetate) of Simarouba glauca were screened for antibacterial activity against five pathogenic microorganisms by well diffusion method. In vitro antioxidant activity of extract was studied using H2O2 radical scavenging assay. The haemolytic activity was determined using agar diffusion techniques on blood agar plate, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis. Results: Among the different extracts tested, the methanol extract of leaves showed significant antimicrobial activities. The most susceptible micro-organisms were found to be Gram negative bacteria (Stenotrophomonas maltophilia, Citrobacter), Gram positive bacteria (Enterococcus faecalis). H2O2 scavenging activity of Simarouba glauca was found to increase with increasing concentration of the extract. IC50 values of H2O2 scavenging activity was 6.72±0.1 µg/mL which was found in chloroform extract. The haemolytic activity was found to be higher in ethyl acetate extract than methanol, chloroform. The chloroform and ethyl acetate extracts shows 23.68...
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Posted by admin on Feb 1, 2014 in |
The objective of the current study was to develop and optimize sublingual tablets of Terazosin Hydrochloride, which is an effective drug in the treatment of Benign Prostate Hyperplasia, Hypertension. Sublingual tablets of Terazosin Hydrochloride were prepared by direct compression method using different superdisintegrating agents such as Crosspovidone, Sodium starch glycolate and Crosscarmellose sodium. The tablets were evaluated for pre-compression studies like Bulk density, Tapped density, Carr’s index, Hausner’s ratio and post-compression studies like Thickness, Hardness, Weight variation, Friability, drug content, Wetting time, Water absorption ratio, in-vitro disintegration time, in-vitro dispersion time, in-vitro dissolution study and also drug release kinetic study. The Hardness, Weight variation, Thickness, Friability and Drug content of tablets were found to be acceptable according to pharmacopoeial limits. An optimized formulation i.e. F6 was found, which provided short wetting time of 67sec, water absorption ratio of 39.01 , in-vitro disintegration time of 61sec and in-vitro dispersion time of 112sec. From the above results It indicated that the amount of superdisintegrant i.e. Crosspovidone was significantly affected the dependent...
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Posted by admin on Feb 1, 2014 in |
A reverse phase high performance liquid chromatographic method was developed for the estimation of paracetamol, guaiphenesin, phenylephrine HCl, chlorpheniramine maleate and bromhexine HCL in a single tablet dosage form. This method was proved to be simple, accurate, precise and robust. The chromatographic separation was achieved on a symmetry C-8 column with dimensions of 150 X 4.6mm, 3.5µm. All the five components were separated by the gradient elution of the mobile phase A consisting of buffer 10mM KH2P04 and 3.7mM of an ion pair reagent, octane-1-sulphonic acid sodium salt. The pH of the mobile phase A was adjusted to 4.0 with ortho phosphoric acid and the mobile phase B consisted of a mixture of methanol and acetonitrile in the ratio of 3:2. The wavelength of absorption was 220nm and the flow rate was fixed at 1.0 ml/min. The retention times of paracetamol, guaiphenesin, phenylephrine HCL, chlorpheniramine maleate and bromhexine HCl were found to be 5.73, 17.46, 18.29, 21.78, and 23.55 respectively. The method was validated according to the ICH guidelines...
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Posted by admin on Feb 1, 2014 in |
Metal Complexes of lanthanum (III), Thorium (IV) and dioxouranium (VI) with Schiff base ligand ethyl 2-{[(2E, 3Z)-4-hydroxypent-3-en-2-ylidene] amino}-4, 5, 6, 7-tetrahydro-1-benzothio-phene-3-carboxylate were prepared. All synthesized complexes were identified and confirmed by elemental analysis, molar conductance measurements and spectral analysis (UV-visible, IR and 1H NMR). The conductance measurement suggested the non-electrolyte nature of the complexes and they were isolated in 1:2 (M: L) ratio. The thermal behavior (TGA/DTA) of the complexes was studied and kinetic parameter was determined by Coats-Redfern method. The data from thermo gravimetric analysis clearly indicates that decomposition of the complexes proceeds in two or three steps .The decomposition of all the complexes ended with the metal oxide. These metal complexes also showed its potential as an antibacterial agent against pathogenic strains causing urinary tract...
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Posted by admin on Feb 1, 2014 in |
Recent evidence indicates that fish, an extremely valuable resource, are quickly becoming scarce. One consequence of this scarcity is the increasing concern for fish survival and a growing interest in identifying the levels of various chemical pollutants, which are safe for fish and other aquatic life. Pesticides are among the most hazardous chemicals to men and ambient. Insecticides are extensively used to protect agricultural crops against the damages caused by pests. However, these chemicals may reach other ecological compartments as lakes and rivers through rains and wind affecting many other organisms away from the primary target. Biochemical and physiological variables show specific responses to certain type of environmental stress. The insecticide Encounter (Herbal plant extract) is used for the present study. The fishes were exposed to different concentrations of insecticide ‘Encounter’ to calculate the LC50 value. The LC50 value is 0.11ppm. Four groups of fishes were exposed for 24, 48, 72 &96 hours respectively. At the end of each exposure period, fishes were sacrificed and tissues such as liver,...
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