Posted by admin on Jan 1, 2014 in |
To investigate an Ayurvedic compound formulation Phalatrikadi Kvatha (PTK) having seven plant origin drugs for quality control and standardization. This compound formulation is mentioned in Ayurvedic classics for the treatment of Kamala ~ hepatic disorders. Macroscopy, qualitative phytochemical analysis, extractive values in ethanol and water and thin layer chromatography (TLC) of the formulation was done. Physicochemical parameters include loss on drying, total ash, acid insoluble ash, sulphated ash, alcohol soluble extractive, water soluble extractive and preliminary phytochemical screening of various solvent extract (hexane, chloroform, ethyl acetate, methanol and aqueous). Phytoconstituents such as Tannins, Saponin, Flavonoids, and Steroids were found to be present. Further TLC of Alcoholic extract of Phalatrikadi Kvatha (PTK) was done for proper identification. The qualitative phytochemical and pharmacognostic study of the drug is helpful in sample identification, quality and purity standards of the plant materials. The result of the present study can serves as valuable source of information and provides suitable standards for identification of PTK formulation for future investigation and its...
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Posted by admin on Jan 1, 2014 in |
Floating matrix tablets of ranitidine hydrochloride were developed and evaluated to increase bioavailability by increasing gastric residence time and sustained release of drug in the upper part of gastrointestinal tract thereby diminishing side effects and enhanced patient compliance. The tablets were prepared by direct compression method, using polymers such as Hydroxy propyl methyl cellulose, carbopol 940, xanthum gum, oryza sativa husk, chitosan and cetyl alcohol. The formulations were evaluated for various physical parameters, buoyancy studies, dissolution parameters and drug released profile from all formulation batch F8 showed slow and sustained release of ranitidine hydrochloride over a period of 12 hours upto 95%. Optimized floating matrix tablets F8 showed no change in physical appearance, drug content, or in dissolution pattern after storage at 40±2°C/75±5% for 90 days. It was concluded that formulation F8 shows the better buoyancy and drug...
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Posted by admin on Jan 1, 2014 in |
A simple, accurate rapid and precise RP-HPLC method has been developed and validated for determination of Hamycin and Ketoconazole in Pharmaceutical Cream. The RP-HPLC separation was achieved on Thermosil C-18, (250 mm, 4.6 mm, 5μm) using mobile phase 0.4 % ( v/v) diisopropylamine in methanol (v/v): 0.5% (w/v) Ammonium acetate in distilled water (90:10 % v/v) pH 6.5 adjusted with Glacial acetic acid at flow rate of 1.0 ml/min at ambient temperature. The retention times were 2.433 min. for Hamycin and 4.711 min. for Ketoconazole. Calibration plots were linear over the concentration range 50-250 μg/ml for Hamycin and 200-1000 μg/ml. Quantification was achieved with UV detection at 263 nm over the Beer-Lambert’s range.The method was validated statistically and applied successfully for the determination of Hamycin and Ketoconazole. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for the routine determination of Hamycin and Ketoconazole in pharmaceutical cream. The proposed method was validated as...
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Posted by admin on Jan 1, 2014 in |
The main objective of the present investigation is to evaluate theantilithiatic activity of ethanolic extract of Tamarindus indica L. on mice. Antilithiatic activity of the ethanolic extract of the Tamarindus indica leaves at a dose of 250mg/kg, 400mg/kg & 500mg/kg was evaluated against the standard drug Cystone was given orally 750mg/kg. Adult Wister Albino rats of either sex of divided in six groups of six animals each as undertaken for study and evaluated by Rat models of calcium oxalate urolithiasis induced by either ethylene glycol (EG) are most commonly used to study the pathogenesis of urolithiasis. Ethanolic extract of Tamarindus indica (at 250, 400 and 500 mg/kg) exhibited a dose dependent significant anti-lithiatic activity on treatment. The extract dose of 250 mg/kg also caused partial reduction of Uria, Uric acid, calcium, Potassium, oxalates, phosphorus and creatinine in blood serum level the results were found statistically insignificant. The antilithiatic effect of ethanol extract at was found less effective than the reference...
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Posted by admin on Jan 1, 2014 in |
The aim of the present study was to investigate the oil composition extracted from Sesamum indicum seeds. The oil was extracted from seeds by soxhlet method and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 84 different components were identified and out of which 12 are fatty acid components. The principal compounds identified and present in all samples were silane, trimethyl[5-methyl-2-(1-me thylethyl)phenoxy]-; silicic acid, diethyl bis (trimethylsilyl)ester; stearic acid; oleic acid; n-Hexadecanoic acid; myristic acid; indole-2-one 2,3-dihydro-N-hydroxy-4-methoxy-3,3-dimethyl-; cyclotrisiloxane, hexamethyl-; acetamide, N-[-4-(trimethylsilyl)phenyl]-; benzene, 2-[(tert-butyldimethylsilyl)oxy]-1-isopropyl-4-methyl-; benzo[h]quinoline, 2,4-dimethyl-, trichloroacetic acid undec-2-enyl ester and 2-Ethylacridine. Among fatty acids, palmitic acid and oleic acid were identified in highest concentration. In comparison, myristic acid and stearic acid were present in lesser amount. Variation was found between chemical compositions of different genotypes of sesame. The obtained results supported the use of seed oil as edible oil and in pharmaceuticals. This study is also important as identification of volatile compounds in Indian genotypes of Sesamum indicum was done for the first...
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