Posted by admin on May 1, 2014 in |
A fast simple sensitive precise, accurate and reproducible RP-HPLC method was developed and validated for the analysis of rivastigmine hydrogen tartrate in transdermal drug delivery system. Rivastigmine tartrate is soluble in water so it was used as solvent. The separation was conducted by using C-18 RP-HPLC column which was maintained at ambient temperature.The mobile phase consist 0.01M ammonium acetate buffer and acetonitrile (70:30 v/v) was delivered at a rate of 1ml/min. The analysis was detected by using UV detector at the wavelength 219nm. The method is validated for its accuracy precision,ruggedness, linearity and range. The method was found to be linear over the concentration range 50-100 µg/ml (r2 = 0.999). The retention time for rivastigmine was found to be 4.40min.The total run time of chromatogram was about 10min.The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. The above method was a rapid and cost-effective quality-control tool for routine analysis of Rivastigmine hydrogen...
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Posted by admin on May 1, 2014 in |
The spread of antibiotic resistant pathogens is one of the most serious menaces to successful treatment of microbial diseases. Medicinal and aromatic plants are widely used as traditional medicines and constitute a major source of natural organic compounds. Semecarpus anacardium Linn (Family: Anacardium), is a plant well known for its medicinal value in Ayurvedic and Siddha system of medicine. The plant was selected on the basis of its reported ethnobotanical uses. The present investigation was undertaken to evaluate the phytochemicals and antifungal activity of methanolic extract of Semecarpus anacardium L. nuts oils. The preliminary phytochemical studies showed the presence of alkaloids, saponins, tannins, flavonoids, steroids, glycosides. Different Concentration of methanolic extract (6.25, 12.5, 25, 37.5, 50, 62.5 μg/ml) of S. anacardium were tested against four fungal strains namely Fusarium oxysporum, Rhizctonia solanii, Alternaria spp., and Sclerotium rolfsii. The excellent inhibitory activity was observed against Rhizoctonia solanii (100%) followed by Sclerotium rolfsii (92.59 %), Alternaria spp. (72.34 %) and Fusarium oxysporum (47.19 %) at 62.5 μg/ml. Among different fungi tested R. solanii and Sclerotium rolfsii were found to be more...
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Posted by admin on May 1, 2014 in |
The present study was aimed to study the analgesic potency of some new pyrazole compounds. Various Quinazolinone clubbed Pyrazole derivative compounds (7a1, 7a2, 7b1, 7b2) were synthesized by using anthranilic acid as starting material as per standard chemical procedure. The synthesized compounds were characterized by physical and spectral analysis by using UV-visible spectrophotometer, Fourier transform infra red (FTIR) Spectrophotometer, 1H Nuclear Magnetic Radiation spectrometer and Gas Chromatography Mass Spectophotometer.-QC-2010. Moreover Pyrazole derivatives have found their clinical application as analgesic agent. In this study the synthesized novel Pyrazole derivatives were screened for in-vivo analgesic properties by tail-flick method. The result obtained is compare with standard diclofenac. It has been observed that the maximum analgesic activity was recorded by the compound 7a1 having 359 % analgesic activity followed by compound 7b1 (268 %), 7b2(204 %) & 7a2 (159%) analgesic potential. The compounds 7a1showed nearly similar percentage analgesic activity compaired to standard drug Diclofenac sodium, whereas 7b1, 7b2 & 7a2 registered percentage analgesic activity relatively less than standard drug. The compound 7a1possess...
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Posted by admin on May 1, 2014 in |
In this present study, we investigated phytochemical constituents screening and In-vitro antioxidant activity of hydro-alcoholic (methanol 70%v/v) extracts of Soymida febrifuga bark. Soymida febrifuga is a huge tree bearing deciduous foliage and having a tough bark belonging to the family Meliaceae. Traditionally the different parts of plant such as root, leaves, bark, and flower are used for various human ailments. Thebark extracts are used in treatment of rheumatoid arthritis asthma and good for ulcers .The decoction of the bark has bitter resin used in vaginal infections, rheumatic pains , stomach pains, wounds, dental diseases, uterine bleeding and haemorrhage .The bark is also used as an acrid, refrigerant, laxative, good for sore throat, removes ‘vata’ and cures ‘tridosha’ in Ayurveda. Apart from many uses various active constituents like methyl angolensate, luteolin 7-O-glucoside, quercetin, sitosterol, myrecetin were isolated. It also possesses various pharmacological activities such as anticancer, antihelmenthic, antioxidant ant malarial and antimicrobial. In view of this the hydro alcoholic bark extract of S. febrifuga produced a dose dependent inhibition of...
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Posted by admin on May 1, 2014 in |
Context: Malaria endemic disease remains the most common tropical parasitic disease in the world. According to the World Health Organization (WHO), malaria is the first endemic disease of which humanity is paying a heavy price. Malaria is a major public health problem in Côte d’Ivoire. Objective: To evaluate the in vitro chemosensitivity to Lumefantrine (LUM) and analyze Pfmdr-1gene polymorphism in isolates of Plasmodium falciparum from Abidjan. Materials and Methods: The in vitro activity of Lumefantrine (LUM) was performed using the optical variant of microtest of the World Health Organization (WHO). After extraction, plasmodial falciparum DNA fragments were amplified by PCR method. The study of Pfmdr-1 polymophism was performed after sequencing of amplicons. The analysis of the relationship between the observed mutations and chemosensitivity of isolates was performed using Cohen’s kappa test. Results: Out of 64 Plasmodium falciparum isolates tested, 57 (89%) gave cleare results of in vitro culture. It was found that 67% of them were susceptible to LUM against 33% that were resistant. Molecular study performed on 28...
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