Posted by admin on Mar 1, 2014 in |
The study was carried out to assess the antibacterial & cytotoxic activities of extracts from leaf parts of the plant, Averrhoa bilimbi. Ethanol, methanol and water were used as solvents and antibacterial & cytotoxic effects were measured using disc diffusion test & brine shrimp lethality bioassay (BSLB). The susceptibility of the microorganisms to the extracts of this plant was compared with standard antibiotic cefadroxil. Water extract exhibited more potent antimicrobial activity by inhibiting wide range of gram positive and gram negative bacteria in comparison to ethanolic and methanolic extract of leaves of A. bilimbi. On the other hand, the ethanolic extract of A. bilimbi leaves( EEL) was found to be the most toxic to Brine Shrimp nauplii, with LC50 of 3.7 μg/ml whereas anticancer drug vincristine sulfate (VS) proved LC50 value 1.73 μg/ml indicating that the potent cytotoxic compounds in this plant have affinity for non- polar solvents. The spectrum of activity observed in the present study may provide the indication that the plant could be a possible source...
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Posted by admin on Mar 1, 2014 in |
A simple, rapid, accurate, precise and economical RP- HPLC method with UV detection was optimized, developed and validated as per ICH-Q2 guideline for the simultaneous estimation of Esomeprazole magnesium trihydrate (ESO), Itopride hydrochloride (ITO) and Mosapride citrate (MOSA) in laboratory sample. An optimum condition of separation and detection was developed on a reverse-phase Supelco 516 C18 DB column (250mm×4.6mm i.d., 5µ particle size), using a mobile phase composition of phosphate buffer (20mM, pH-7.4 adjusted with sodium hydroxide):acetonitrile:methanol in the ratio of a 20:20:60 (%v/v) at a flow rate of 1.0 ml/min with UV detection at 275 nm within 6 min with retention time of 3.09, 3.89 and 5.19 for ESO, ITO and MOSA respectively. The standard curves were linear over the concentration range of 2-12 μg/mL, 7.5-45 μg/mL and 1.5-9 μg/mL for ESO, ITO and MOSA respectively with R2 more than 0.999. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification. From the validation results it was concluded that proposed method...
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Posted by admin on Mar 1, 2014 in |
In this study, aspirin (AS) and isosorbide 5-mononitrate(ISM) sustained bilayer tablets were developed by wet granulation and compression technique, the influence of the contents of PVPP on the dissolution of AS from the tablets was investigated. An orthogonal experiment design was used to optimize ISM sustained release layer. The tablets were tested for their drug content, hardness, thickness, friability, and in vitro release characteristics. The optimized formulation of sustained bilayer tablets contains ISM 60 mg, HPMC K100M 45 mg, CMC-Na 12 mg, HPMC K15M 12 mg, lactose 50 mg and aerosol 3 mg for sustained release layer of ISM, PVP K30 Ethanol solution was used as adhesives. The optimized formulation of sustained bilayer tablets contains AS 75mg, PVPP 10mg, citrate acid 15mg, MCC 35mg, pregelatinized starch 15mg and 2mg talc for fast release layer. The drug release of AS was above 80% at 0.5h hour and ISM was above 70% at 7h in the optimized...
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Posted by admin on Mar 1, 2014 in |
Jasminum multiflorum belongs to the Oleaceae family. It is used as a traditional medicine from ancient times. It is a large evergreen twinner shrub found throughout India. Present study reports the phytochemical properties which have been carried out on the leaves extract using ethanol, methanol, ethyl acetate, acetone and n-hexane. The phytochemicals analysis show the presence of Phenols, Saponins, Terpenoids etc; TLC has been carried out on different extracts of leaves of Jasminum multiflorum, which have shown different Rf values. The presences of phenols, terpenoids and saponins based phytochemicals are...
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Posted by admin on Mar 1, 2014 in |
The aim of the present work is to increase the gastric residence time of Lamivudine by preparing it into gastro retentive floating tablets whereby making it available at its site of absorption and to achieve an extended action for a time period of 24hrs and to reduce the dosing frequency to once a day, using release retardant polymers METHOCEL, POLYOX and CARBOPOL. The compatibility of the drug with excipients was confirmed by DSC study. The gastro retentive floating tablets were prepared by direct compression method and were evaluated for physicochemical characteristics and in-vitro dissolution studies. The optimized formulation was subjected to stability studies. The prepared tablets exhibited satisfactory physicochemical characteristics. The in-vitro drug release studies revealed that the drug release was sustained up to 24hrs for formulation F5 containing drug and POLYOX as release retardant polymer at a concentration of 1:0.75. Using Higuchi’s Model and the Korsmeyer equation, the drug release mechanism from the floating sustained release tablets was found to be Anomalous (non-Fickian) diffusion. Optimized formulations showed no...
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