Posted by admin on Feb 1, 2014 in |
The long-term effects of daily consumption of benzene-contaminated water over a period of Sixty-five days on rats’ cellular system were inspected. Haematological parameters such as haemoglobin, packed cell volume etc were determined. Standard enzyme assays were conducted on some enzymes (alkaline phosphatase, acid phosphatase and lactate dehydrogenase) activities of the heart and serum. Significantly lower (P<0.05) enzymes activities were observed in the heart of test rats relative to control. Conversely, enzymes activities in the serum significantly increased. The white blood cell of test rats was found to be 11.3±0.5 while that of control was 9.8±0.6 suggesting infection in the test rats. Haemoglobin, red blood cell count and packed cell volume of test rats were found to be significantly lower (P<0.05) than that of control which may be indicative of anaemia. It could be inferred therefore, that consumption of benzene-contaminated water could pose serious health problems such as heart failure and abnormal haematological...
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Posted by admin on Feb 1, 2014 in |
The present investigation was under taken to find out the effect of stem bark extracts of medicinally important forest tree Oroxylum indicum (L) Kurz on silk production of Bombyx mori. Silk worms were fed with the leaves of mulberry fortified with stem bark extracts of O. indicum. The silk worms were reared up to the end of fifth instar. As the concentration of stem bark extract of O. indicum increased, there was significant increase in the average cocoon weight, pupal weight, shell weight, silk percentage and also filament length of silk. Hence, the stem bark extract of O. indicum can also be considered as cheap and abundant source to enhance the commercial characters of...
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Posted by admin on Feb 1, 2014 in |
The study describes development and subsequent validation of a reverse phase (RP) high-performance liquid chromatography (HPLC) method for the estimation of sparfloxacin (SPR) in tablet dosage form. The liquid chromatographic separation was achieved isocratically using a mobile phase composed of phosphate buffer (5.3 mM) and acetonitrile in the ratio of 60:40 (v/v) adjusted to pH 3.5 using dilute phosphoric acid. The analysis was carried out using X terra column (4.6 ´ 150 mm, 5µm, C18) at a flow rate of 0.5 ml/min and the UV detection at 298 nm. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness and selectivity. The linearity of the proposed method was investigated in the range of 4 to 24 µg/ml (r2 =0.999). Quantitative and recovery studies of the dosage form were also carried out and analyzed, the % RSD from recovery studies was found to be within the limit. Due to simplicity, rapidity and accuracy of the method we believe that the method will be useful for routine quality control...
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Posted by admin on Feb 1, 2014 in |
The purpose of this study was to develop and optimise the formulation of mucoadhesive patches of Diltiazem hydrochloride using chitosan as base matrix. The patches were prepared by solvent casting method. PVP (K30) and propylene glycol were introduced into the patches formulae to improve the filming properties. The patches were found to be smooth in appearance, uniform in thickness, weight, and drug content and also showed good mucoadhesive strength and folding endurance and moderate swelling properties. The permeation study of the patches indicated a drug release of 80% in 10 hrs. The data analysis of the release profile using Peppas model indicated a non Fickian type of release profile. It can be concluded that buccal patch of Diltiazem hydrochloride can be an alternate for improving bioavailability with a control release...
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Posted by admin on Feb 1, 2014 in |
Tiemonium Methylsulfate is an antispasmodic drug. It strengthens calcium bonding with phospholipids and proteins. A simple, sensitive and highly accurate UV spectroscopic method has been developed for the determination of Tiemonium Methylsulfate in bulk and its tablet dosage form. Tiemonium Methylsulfate in its distilled water solutions was determined at the wavelength range of 200-400 nm by the spectroscopic method. Solution of Tiemonium Methylsulfate in distilled water shows a maximum absorbance at 234 nm. Beer’s law was obeyed in the concentration of 10-30µgmL-1, correlation coefficient, detection and quantification limit were also calculated. The proposed method has been applied successfully to assay of Tiemonium Methylsulfate in pure and tablet dosage form. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentage of assay of Tiemonium Methylsulfate in tablet was 99.90-100.12% of the label claimed 50 mg per tablet. The method was then validated statistically as per ICH guidelines which yielded good results concerning range, precision, accuracy, reproducibility, specificity and...
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