Posted by admin on Nov 1, 2013 in |
From this current research, Syk (spleen tyrosine kinase) protein and gene information is analyzed by different genomics, proteomics tools & databases. One crystal ligand 4DFL was collected from protein data bank (pdb). From different literature review 131 syk protein inhibitors were collected. Molecular modeling of these 131 molecules was done through Accelrys discovery studio (ADS). Choose appropriate force-field & minimization (Smart, Stephent Descent, and Conjugate Gradient) according to selected molecules. Then collected crystal ligand is purified by protein purification method and used appropriate conformation (BEST, FAST, and CAESAR). Docking methods were analyzed with protein, crystal ligand and similar inhibitors to know the best protein-ligand interaction. Pharmacophore research is done through HIPHOP and HYPOGEN method. Protein with final compound docking method is done after completion of virtual screening method. Pharmacophore research with final molecule was done. Quantitative structure activity relationship (qsar) method is analyzed to know the correlation between the above selective structures. From virtual screening method, best and final compound is analyzed. So, final molecule can be a drug...
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Posted by admin on Nov 1, 2013 in |
A simple, accurate, sensitive and reproducible UV spectrophotometric method has been developed for the determination of Tulobuterol (TLB) in bulk and also in its pharmaceutical dosage formulations. The proposed method showed absorbance maxima at 212 nm. Beer’s law is obeyed over a concentration range of 25-125µg/mL. The respective linear regression equation being Y =0.009x +0.014 for TLB. Results of analysis for the method established, was validated statistically and also by recovery studies. The apparent molar absorptivity and Sandell’s Sensitivity values are 0.43×104 Lmol-1cm-1 and 0.0371 µgcm-2 respectively. The assay and recovery studies were found to be 99.16% and coefficient correlation(r) was found to be 0.999. The different experimental parameters effecting the development and stability were studied carefully and optimized. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were also obtained. The developed method employed was successful for the determination of TLB in various pharmaceutical...
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Posted by admin on Nov 1, 2013 in |
Thyme (Thymus vulgaris L.) is a pleasant smelling perennial shrub, which grows in several regions in the world. The plant is reported to have high economic and medicinal values. The objectives of this research was to determine antimicrobial activity of petroleum ether, chloroform and hydroalcoholic extracts obtained from leaves of Thymus vulgaris collected from Oman. The other objective was to determine cytotoxic activity of the three extracts. Antimicrobial activity was measured using disc diffusion method against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia. Brine shrimp test was used to estimate cytotoxic activity. In antibacterial assay, Thymus vulgaris leaves extracts showed very strong results, inhibition zones ranged from 7 – 20 mm. So this plant can be used as a good source of potential antimicrobial agent.Petroleum ether showed the highest antibacterial activity. Furthermore, Petroleum ether and Chloroform extracts have almost killed all the shrimp larvae at higher concentration of 1000 mcg/ml. LC50 values for the two extracts were 85.2 and 95.8 mcg/ml, respectively. Polar fractions like hydro alcoholic...
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Posted by admin on Nov 1, 2013 in |
In different parts of India, Bombax malabaricum (Family: Bombacaceae), is well known for its traditional use in inflammation, prolapse of uterus or vagina, menorrhoea, diarrhoea, wound etc. Literature survey showed that the leaves of Bombax malabaricum have anti-inflammatory, antipyretic, analgesic and antioxidant activities. Although the plant has much potentiality, proper pharmacognostical standardization of the parts of the plant was still deficient. The aim of the present research work was to carry out a systematic pharmacognostical and phytochemical evaluation of Bombax malabaricum leaves. After collection, the leaves were authenticated and evaluated pharmacognostically to establish its macroscopical and microscopical characters and also that of the powdered leaves. In the microscopical features of Bombax malabaricum leaves, full of mucilage containing cells and numerous calcium oxalate crystal containing mesophyll tissues were seen. In the powder microscopy, stone cell, reticulate type xylem vessels, multicellular covering trichome and glandular trichome were seen. In preliminary phytochemical screening, the methanol extract of the leaves showed the presence of steroids, carbohydrates, tannins, triterpenoids, deoxysugar, flavonoids and coumarin glycosides....
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Posted by admin on Nov 1, 2013 in |
Background: Skin wound infected by Staphylococcus aureus (S. aureus) is a common and painful lesion. Tetracycline is commonly used as the antibiotic of choice for its treatment. Aim: to determine the effectiveness of hen egg white lysozyme and dextran conjugated lysozyme in decreasing the bacterial count of experimentally induced skin wound infection in mice. Methods; Lysozyme was conjugated with dextran under mild Maillard reaction conditions. Lysozyme and conjugated lysozyme were tested in vitro for their effectiveness against S. aureus and E. coli. Mouse model of skin wound infection was prepared in 6 group of mices (n=5) by 1cm × 1cm paravertebral laceration, extending to fascia created by scalpel. Five minutes after wounding, 10 µl culture media suspension containing 106 CFU⁄cm2 S. aureus was inoculated. Lysozyme and dextran conjugated lysozyme were applied as a 400µg/ml eucerin ointment base in treatment groups. Tetracycline was applied topically in the third group of mice. Results: Both Lysozyme and conjugated lysozyme exhibited in vitro antibacterial effect against S. aureus at 400µg/ml, but only the...
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