Posted by admin on Apr 1, 2014 in |
The objective of the present study was to develop Sustained release matrix tablets of Dicyclomine hydrochloride to reduce the dosing frequency to twice daily thereby increasing patient compliance and therapeutic efficacy. Twelve batches of tablets were fabricated using hydrophilic polymer guar gum alone or in combination with xanthan gum and pectin. All the batches were formulated by wet granulation and evaluated for thickness, weight variation, hardness, drug content uniformity, swelling index and in – vitro drug release profile. The results obtained were satisfactory and complied with the Pharmacopoeial specifications. From the twelve batches, B9 formulation had better control over release rate and can increase patient compliance through twice daily dosing. In- vitro drug release for B9 formulation containing 1:3 drug: polymer (guar gum and pectin) ratio was found to be 18.21% at the end of 2nd hour and 81.26% at the end of 11th hour. The dissolution data was fitted into various models to determine the mechanism of drug release. Kinetics of drug release of the optimized batch showed...
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Posted by admin on Apr 1, 2014 in |
Tuberculosis (TB) is a disease caused by a bacterium called Mycobacterium tuberculosis. The bacteria usually attack the lungs. Tuberculosis is a global health threat and infecting about one third of the human population. Despite having a variety of anti-tuberculous drugs and availability of effective chemotherapy and Bacille-Calmette –Guerin (BCG) vaccine, tuberculosis remains a leading infectious killer world-wide. This has prompted an urgent need of new drugs and the identification of new drug targets. GlmU is a bifunctional acetyltransferase/uridyltransferase that catalyses the formation of UDP-GlcNAc from GlcN-1-P. UDP-GlcNAc is a substrate for two important biosynthetic pathway: lipopolysaccharide and peptidoglycan synthesis. The glmU protein is essential in Mycobacterium tuberculosis, being required for optimal bacterial growth. Since inhibition of GlmU affects peptidoglycan synthesis which often results in cell lysis, M. tuberculosis GlmU is a potential anti-tuberculosis drug target. The primary protein sequence analysis was done using protparam tool and secondary structure prediction was done using SOPMA. The NTP_transferase domain and IspD domain found in GlmU protein were identified by SMART and its 3D structure prediction was done using modeller....
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Posted by admin on Apr 1, 2014 in |
Helicteres isora (L.), a South-Asian plant, is a rich source of medicinal and antioxidant compounds and has been widely used in traditional medicine. Antioxidants are important to neutralize damaging free radicals in the body, especially when the systemic defence mechanisms prove insufficient. The objective of this study was to determine the antioxidant potential of its fresh and dry plant parts in various solvent systems. Plant material was collected from Karnala forest of Maharashtra. Extracts of leaves, bark, root and fruits (fresh and dry) were prepared using four different solvents viz. Distilled water, Ethanol, Methanol and Acetone. Each extract was tested for total phenolic content, total flavonoid content, and antioxidant activity by – FRAP, DPPH˙ and ABTS˙+ assays while phenolic compounds like Gallic acid, Cathechol, Vanillin, Caffic acid, p-Coumaric acid and Ferulic acid were detected using RP-HPLC. Antioxidant potential was significantly high in dried plant parts than the fresh ones. Leaves showed highest phenolic and flavonoid content (11.47 ± 0.50 mg GAE/g) and (54.16 ± 1.22mg RE/g) with 90.21 ±...
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Posted by admin on Apr 1, 2014 in |
Present studies was undertaken to find out of aquatic fungi in river Narmada. Fungi are eukaryotic, heterotrophic organisms, including both single-celled yeasts and multi-cellular filamentous fungi. Many fungal species can survive in oligotrophic environments, through scavenging nutrients from the substrate which they colonies, or the air or water in which they live. Fungi also produce secondary metabolites, some of which are toxins. Some of the fungal species and the metabolites they produce are human pathogens or allergens. It is an attempt for water quality studies by the analysis of aquatic fungi at three sampling sites in...
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Posted by admin on Apr 1, 2014 in |
A simple and precise RP-HPLC method was developed and validated for the determination of Choline fenofibrate in pharmaceutical dosage forms. Chromatography was carried out on Agilent make Zorbax C18 column (4.6 mm x 15 cm), 5 µ particle size using a mobile phase of phosphate buffer (pH 6.8) : acetonitrile (70 : 30 % v/v) at a flow rate of 0.8 ml/min. The analyte was monitored using UV detector at 298 nm. The retention time was found to be 3.207 min for Choline fenofibrate. The proposed method was found to be having linearity in the concentration range of 5-35 µg/ml with correlation coefficient 0.999. The mean recoveries obtained for Choline fenofibrate were in the range of 99.4-100.65 %. The developed method has been statistically validated according to ICH guidelines and found to be simple, precise and accurate with the prescribed values. Thus the proposed method was successfully applied for the estimation of Choline fenofibrate in routine quality control analysis in bulk and its...
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