Posted by admin on Nov 1, 2013 in |
The purpose of this work is to evaluate the possibility of enzyme therapy through microencapsulation of serratiopeptidase (SP) in biodegradable nanoparticles of chitosan (CS). This drug has short biological half-life and thus frequent administration makes it a suitable candidate for controlled release. In this study, serratiopeptidase loaded chitosan nanoparticles were prepared by ionotropic gelation of CS with tripolyphosphate (TPP) anions. Reversible physical cross-linking by electrostatic interaction, instead of chemical cross-linking, has been applied to avoid the possible toxicity of reagents and other undesirable effects. The enzyme loaded particles optimized formulation was coated with sodium alginate solution to protect its release in stomach. The enzyme loaded nanoparticle formulations were characterized for by morphology, particle size, encapsulation efficiency and in-vitro drug release. The preliminary studies show that TPP and CS were compatible with SP. The ratio of CS to TPP has an influence on the mean particle size and when CS: TPP is 4:1 nanoparticles with smallest diameter are formed. Entrapment efficiency depends on the degree of deacetylation of chitosan. The...
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Posted by admin on Nov 1, 2013 in |
Ras proteins in association with GTP and GDP act as a bio-molecular switch for signaling cell growth, cell survival and signal transduction. The presence of mutated Ras proteins is found to vary in different cancer types and the highest occurrence of about 90% is observed in pancreatic cancer. The Ras GTPase binding site is mainly involved in signal cell proliferation. Hence, this binding site has been considered as a major target. At the same time, targeting a specific protein and designing the drug molecule with respect to that is practically of no use as the target proteins are fast mutating. In this scenario, designing the template from the hot spot of proteins and fitting the template for all the target protein molecules seem to be a promising technique. The templates are initially screened on the basis of pharmacokinetic and pharmacodynamic requirements. Six templates are found to be satisfying conditions like IC50, lipophilic efficiency, ligand efficiency etc. and their efficiencies are compared with standard reference molecules. The computed enrichment factors...
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Posted by admin on Nov 1, 2013 in |
Herbal medicines have been broadly used in developed countries hence they are natural and comparatively safe. They contain plant materials as their pharmacologically active components. Infertility is one of the most extremely tragic all over the world. Despite recent advances in the treatment of male infertility, the problem has not been satisfactorily tackled. The male infertility is mainly due to an inadequate number of spermatozoa in the semen, the failure of the spermatozoa to move with sufficient vigor towards their goal. Aim of the study is to evaluate the acute and sub-acute toxicity of the spermatogenic siddha drug Isappukol Chooranam (IC) (siddha drug).For acute studies, different doses of IC were administered orally to rats once daily for one week. Forsub-acute studies, different doses of IC were administered orally to rats once daily for 28 days in various doses at 50,100,200 mg/kg of body weight. Detailed hematological, biochemical, necropsy and Histopathological evaluation of organs was performed for all animals. Histopathological analysis revealed that Spleen, Testes, Pancreas, Lung, Liver, Brain, Heart,...
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Posted by admin on Nov 1, 2013 in |
Aim: To compare safety and efficacy of topical quick penetrating solution (QPS) of heparin 1000 IU/ml versus heparin gel 200 IU/g in the management of post infusion superficial thrombophlebitis. Methods: Total 202 patients of early, medium and advance stage ofsuperficial thrombophlebitis were randomized to receive either treatment. Treatments were applied 3 times daily until healing or for a maximum of 7 days. Primary efficacy endpoints were change in length of the venous lesion, proportion of patients with complete healing; while secondary efficacy endpoints included local symptoms, change in the grade of the lesion, global assessment by patients and investigator. Safety of treatment in all patients was evaluated. Results: Mean change in length of the venous lesion from baseline was significantly higher in heparin QPS group as compared to heparin gel on day 3 (P =0.0144). 90.0% patients in heparin QPS group were healed at day 7 which was significantly higher as compared to 65.7% patients in heparin gel group (P=0.00001). Heparin QPS was also found better in secondary efficacy...
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Posted by admin on Nov 1, 2013 in |
An isocratic HPLC method with UV detection at 225nm is described for simultaneous determination of Guaiphenesin, Chlor-pheneramine Maleate & Dextromethorphan HBr. Chromatographic separation of all three drugs were achieved on Purospher star RP-18e column (Merck) using Mobile phase as 74% Buffer (prepared by dissolving 1.8g dipotassium hydrogen orthophosphate in 1 litre of HPLC grade water, adjusting pH-3 with phosphoric acid) & 26% Acetonitrile. Method offers simplicity, good linearity, symmetric peak shape, and good resolution is achieved in a run time of 13 minutes. The method has been validated for Specificity, Linearity, Accuracy, and Precision. Stress testing which covered Oxidation, acid and alkali hydrolysis, photolysis and thermal degradation was performed on individual molecules under test to prove the specificity of the method and the degradation products formed were resolved effectively. It has a very good linearity with correlation coefficient value more than 0.999, % recovery for Guaiphenesin, Chlorpheneramine Maleate & Dextromethorphan HBr obtained were between 99-101%, and the relative standard deviation for 5 replicates obtained was less than...
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