Posted by admin on Jan 1, 2014 in |
A novel, precise, accurate, rapid and cost effective isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed, optimized and validated for the estimation of Arterolane maleate and Piperaquine phosphate in pharmaceutical dosage forms (tablet). The drugs were estimated using hypersil C18 (250 mm x 4.6 mm i.d- 5 µm particle size) column. A mobile phase composed of phosphate buffer, acetonitrile, methanol in proportion of 40:30:30 v/v, at a flow rate of 1.0 ml/min was used for the separation. Detection was carried out at 244 nm. The linearity range obtained was 20-70 µg/ml for ART and 100-350 µg/ml for PIP with retention times (Rt) of 3.353 min and 2.389 min for ART and PIP respectively. The correlation coefficient values were found to be 0.999. Precession studies showed % RSD values less than 2 % for both the drugs in all the selected concentrations. The percentage recoveries of ART and PIP were in the range of 99.47-100.72% and 99.30-100.18% respectively. The assay results of ART and PIP were 99.57% and...
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Posted by admin on Jan 1, 2014 in |
Spirulina is an algae used in our country for people living with HIV (PLHIV) and it is known especially for its therapeutic immunostimulatory effects. Spirulina capsules produced locally enriched in zinc and selenium called “spiruline plus” were formulated and given to PLHIV. The objectives of this randomized placebo controlled trial were to assess the tolerance by reporting side effects and to evaluate the clinical and biological outcome of patients after 12 months follow-up. A randomized clinical trial comparing four groups of patients infected with HIV type 1 (HIV-1) and treated with different diets was conducted over 23 months. These groups were: a first group of patients treated only with placebo, a second group treated with “spiruline plus”, a third group treated with antiretroviral (ART) and placebo, the fourth group treated with ARTs and “Spiruline plus”. Clinical and laboratory data were collected at baseline and at 3, 6, 9 and 12 months after inclusion. Some of the side effects reported in the group receiving antiretroviral therapy and “spiruline plus” decreased...
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Posted by admin on Jan 1, 2014 in |
We report here the optimization of an oxidative Michael addition protocol for the synthesis of highly functionalized, biologically relevant benzofurans on gram scale. Rigorous screening of reaction conditions including solvent, atmosphere, base and incubation time, and development of a straight forward precipitation step afforded benzofurans in greater than 95% purity for direct use in biological...
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Posted by admin on Jan 1, 2014 in |
Furosemide is a potent and commonly used loop diuretic. It is absorbed largely in the stomach and upper small intestine. This narrow absorption window results in its low (average of 50%) and variable (10-100%) bioavailability from conventional dosage forms. The objective of the present study was to develop an optimized controlled release floating microspheres of furosemide capable of floating on the gastric fluid and delivering the drug over a period of 12 h. The floating microspheres were prepared by solvent evaporation method. Preliminary studies were conducted and, drug loading and EC/HPMC ratio were identified as the most important factors affecting the desired response variables: drug release rate and buoyancy. The effects of drug loading and EC/HPMC ratio were further studied and optimized. Simultaneous optimization of buoyancy and release rate was performed using central composite design and the most desirable optimal point was obtained at release rate of 27 h-1/2 and buoyancy of 58.45%, with corresponding levels of 344 mg furosemide and 4.84 EC/HPMC ratio. Evaluation of the optimized formulation...
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Posted by admin on Jan 1, 2014 in |
In the present study, Vigna mungo (blackgram) have been used to investigate bioactive compounds through soxhlet extraction. The obtained methanol extract was evaluated for antimicrobial activity by Kirby-Bauer method against bacterial strains (Enterococci sp., Pseudomonas aeruginosa, Klebsiella sp., Bacillus sp., Staphylococcus aueres)and fungal strains (Aspergillus niger, Candida albicans) on Muller Hinton agar medium and potato dextrose agar medium respectively. The blackgram was analyzed for the presence of proteins through SDS-PAGE and compared with the BSA (66KDa) and Lysine (18KDa) protein markers. The sensitivity of the obtained methanol extract against pathogenic microorganisms was studied with the combined effect of commercially available antibiotics which resulted in good and enhanced antimicrobial effect. The methanol extract of blackgram was also analyzed for bioactive compounds through gas-chromatography mass spectrometry (GC-MS). The GC-MS spectrum revealed bioactive compound with molecular weight of 297.3268 (Heptadecanoic acid, 9-methyl, methyl ester). The active constituents of Vigna mungo was identified through mass spectroscopy analysis act as potential compound for effective antimicrobial and pharmaceutical...
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