Posted by admin on Sep 1, 2013 in |
Simple, sensitive and convenient spectrophotometric methods (method A and method B) are developed for the determination of diclofenac diethylamine (DDEA) in pure form and in formulations. Method A is based on the oxidation of drug by Fe(III) followed by the complex formation between reduced form of Fe(III) with 1,10-phenantroline. The resulting red colored chromogen is measured at 510 nm. Method B is based on the formation of charge transfer complex between DDEA (n-donor) and p-chloroanilic acid (π-acceptor) in 1,4-dioxane. The resulting violet colored chromogen is measured at 526 nm. Beer’s law obeyed in the concentration range of 2.00-8.00 and 20.00-120.00 µg mL-1 for the method A and B, respectively. The molar absorptivity and Sandell’s sensitivity are found to be 0.98 × 104, 1.62 × 103 L mol-1 cm-1 and 3.77 × 10-2, 2.27 × 10-2 µg cm-2 for the method A and B, respectively.The optimum reaction conditions and other analytical parameters are evaluated. Statistical analysis proves that both the methods are reproducible and selective for the determination of DDEA...
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Posted by admin on Sep 1, 2013 in |
An accurate, simple and precise RP-HPLC method for the simultaneous determination of telmisartan and hydrochlorothiazide in pharmaceutical formulations was developed and validated. Chromatographic separation of two drugs was achieved on a Kromasil C18, (250×4.6nm, 5µm) column by using the mobile phase consisted of methanol, tetrahydrofuran and water in the ratio 55:05:40 at a flow rate of 1mL/min and the wavelength of detection was at 234 nm. The retention time for telmisartan and hydrochlorothiazide were found to be 2.3 and 3.8 min respectively. The linearity of the developed method was tested over a concentration range of 50-100 µg/mL and the correlation coefficient (r2) of regression was 0.999 which is almost equal to 1. The limit of quantification was 0.5µg/mL for telmisartan and 0.4µg/mL for hydrochlorothiazide and the limit of detection was 0.1µg/mL for both telmisartan and hydrochlorothiazide. The mean percentage recoveries were 99.2% for telmisartan and 99.8% for hydrochlorothiazide...
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Posted by admin on Sep 1, 2013 in |
This work has been undertaken for the screening and isolation of fibrinolytic protease producing mesophilic bacteria from ten soil samples, collected from slaughter housesvarious regions of Bangalore and used to screen for fibrinolytic protease production by using fibrin plate assay. In the present study, an attempt was made to isolate efficient fibrinolytic protease producing bacteria from diverse environmental samples. Different isolates were screened for possessing the ability to produce fibrinolytic protease. About 5 bacterial isolates were found to be promising to produce fibrinolytic protease. The organisms were tested for various biochemical tests, which leads to their identification as Bacillus cereus, Bacillus circulans, Pseudomonas aeruginosa, Pseudomonas fluorescens and E.coli....
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Posted by admin on Sep 1, 2013 in |
Complexes of Copper (II) halides with steryl derivative of 2- methylbenzimidazole (stmbz), 2-ethylbenzimidazole (stebz) and 2-benzylbenzimidazole (stbbz) of composition CuL2X2 (L = stmbz, stebz and stbbz and X = Cl, Br) have been prepared and characterised. The magnetic susceptibility e.p.r. data, electronic absorption pattern and i.r. spectral studies of complexes indicated distorted octahedral environment of ligand around copper (II) atom. Steryl derivatives and their copper (II) complexes were screened for their antifungal activity in dilute aqueous ethanolic media where bromide complexes show higher antifungal activity than free...
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Posted by admin on Sep 1, 2013 in |
The present study reports an environmentally friendly, microwave-assisted rapid green synthetic method using Saraca indica leaf extract makes a fast and convenient method for the synthesis of silver nanoparticles and can reduce most of the silver ions into silver nanoparticles within 180 sec of reaction time with the aid of microwave. The synthesized nanoparticles were characterized using UV-visible (UV-vis) spectrophotometer, Transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infra-red (FTIR) spectrometry. The nanoparticles were found to be spherical in shape and of 5 to 50 nm in size. The synthesized silver nanoparticles exhibited good antibacterial potential against both Gram positive and Gram negative bacterial strain as measured using well diffusion...
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