Posted by admin on Dec 1, 2013 in |
The present study is carried out with the methanolic extract of leaves of Millettia pachycarpa benth. Traditional use of it inspired us to investigate the blood corpuscular protective power of this plant as it used as blood tonic in Chinese traditional medicine. By the phytochemical screening we have both flavonoids and steroids. There’s also alkaloids are found which have extensive physiologic action embodying analgesic activity. This investigation is made following the most simple, reliable and less time consuming method. As the human red blood corpuscular membrane is similar to lysosomal membranes that influence inflammatory process. Result obtained using methanolic extract of M. pachycarpa have better acceptance as it shows good response in inhibiting hemolysis (34.30%) at highest concentration and these investigation surely stimulate further screening and isolation...
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Posted by admin on Dec 1, 2013 in |
An attempt was made to formulate and evaluate the 18-β-glycyrrhetic acid (GA) transdermal drug delivery system. Preformulation studies on the drug 18-β-glycyrrhetic acid were done which included description, solubility and compatibility studies. Solvent casting technique was used to prepare the transdermal patches. Four formulations were made with 400mg of 18-β-glycyrrhetic acid and by using polymers Hydoxypropyl methylcellulose (HPMC) and Ethyl cellulose (EC) with different concentration of menthol (2%, 5%) as penetration enhancer. Interaction of drug and polymer was confirmed by FTIR studies. Transdermal patches were evaluated for the weight, thickness, percentage moisture uptake, folding endurance, water vapor transmission rate, in-vitro release and in-vivo release studies. This was done for four formulations F1, F2, F3, and F4. The F1 formulation showed a good release profile due to increase in concentration of penetration enhancer. The accelerated short term stability study indicated that the optimized formulation F1 has very good stability at 4 o / 40 o / 60 o for a period of 3...
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Posted by admin on Dec 1, 2013 in |
Herbal medicine refers to the use of any plant’s seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. Kalanchoe pinnata is known as herbal medicine that having many special advantages. The objective of the study was to scientifically assess antimicrobial activity of 95% ethanolic, methanolic, 60% methanolic and aqueous extract of leaves from Kalanchoe pinnata on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. It was found that 60% methanolic extract shows best result. The 60% methanolic extracts of the selected plants leaves were taken in different ratio randomly and antimicrobial tests were carried out. The most effective concentration was then determined by comparing the results of the zone of inhibition. Phytochemical screening of 60% methanolic extract was carried out and the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) was found significant activity against microorganisms. The literature survey show that this plant leaves has only antibacterial activity. But with this research it is conclude that the plant leaves not only have antibacterial activity but also antifungal...
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Posted by admin on Dec 1, 2013 in |
A simple, rapid, accurate, specific and sensitive reverse phase liquid chromatography method (RP-HPLC) was developed and validated for the estimation of Cytarabine in Pharmaceutical dosage form. The method was standardized using a Nova pack C18 column (250 mm x 4.6 mm ID; Particle size 3 μm) and the mobile phase consisted of acetonitrile: buffer (Ammonium acetate) at 30:70% v/v. The eluents were monitored at 272 nm and at 1 ml/min flow rate. The retention time was found to be 2.734 min for Cytarabine. The developed method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantification in accordance with the ICH guidelines. Linearity was obtained with the correlation coefficient value r2 =1 for Cytarabine. The proposed method can be used for the estimation of Cytarabine in bulk and Pharmaceutical dosage...
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Posted by admin on Dec 1, 2013 in |
A simple, precise, accurate and repeatable method for bulk and tablet dosage form of Ritonavir have been developed using spectrophotometric method. Differential spectrophotometric method was used. The developed method was validated according to ICH (Q2R1) guidelines and was found to be accurate, precise and specific. Amplitude difference was taken at absorbance maxima at 246 nm and absorbance minima at 266 nm. Linearity range was found to be within the concentration range of 10-30 μg/mL. Limit of detection and quantification was found to be 2.62 and 7.96 μg/mL respectively. The proposed method was found accurate in the range of 95.5 to 105.5%. It can be successfully applied for the estimation of Ritonavir in bulk and pharmaceutical dosage forms. Results of the analysis were validated statistically and by recovery...
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