Posted by admin on Sep 1, 2013 in |
Ancient DNA help in better understanding the evolution and migration pattern of humans throughout the world. The present work is a preliminary effort, to extract and amplify the ancient DNA from human bone samples provided by the Anthropological survey of India collected from the Himalayan region in 1952. Many technical difficulties arise during DNA extraction, PCR amplification and sequencing which make it difficult to work and interpret the analyses.Our preliminary result is encouraging, as we have successfully amplified 456 base pair PCR product of mitochondrial DNA hypervariable region I (HVR1) starting from position 15976 to 16431. Our results confirm that the ancient bone specimens harbours M haplogroup signature with considerable similarities to M21ab sub-group specific to East and Southeast Asia. Absence ofnegative amplification confirms that our ancient mitochondrial DNA is contamination...
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Posted by admin on Sep 1, 2013 in |
Sodium alginate and combinations of sodium alginate-pectin were used to study the effect on the loading efficiency and the radical scavenging activity of the polyphenols extracted from pomegranate peels (Punica granatum). The results indicate that the polyphenol content was less when the microbeads were prepared with a single type of polymer in comparison of the microbeads prepared with two types of polymers. Also there was an optimum ratio of these two polymers (2:1), which was responsible for the maximum polyphenol content. The microencapsulated particles provided to polyphenols an effective protection against the degradation phenomenon, whereas antioxidant activity remained identical. In-vitro release studies indicated that 64.87% and 48.81% of polyphenols was released in simulated gastric fluid from sodium alginate and sodium alginate-pectin microbeads respectively. 88.37% and 70.48% of polyphenols was released in simulated intestinal fluid from sodium alginate and sodium alginate-pectin microbeads respectively. The microcapsules described in this study represent an interesting food additive for incorporation into functional...
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Posted by admin on Sep 1, 2013 in |
An HPLC method was developed and validated to determine felodipine and related substances. A C18 column (5μm, 250 ×4.6 mm) was used for the separation at room temperature, with methanol: acetonitrile: water (50:15:35%, v/v/v) as the mobile phase at the flow rate of 1.0mLmin−1. The detection wavelength was 238 nm. The validation characteristics included accuracy, precision, specificity, linearity, and robust, and stability-indicating. The method showed good linearity for felodipine and its related substances with correlation coefficients in the range of 5.05-40.4μg/mland 0.31-15.50μg/ml. Method accuracy was assessed for felodipine and its related substances at three levels, the recovery ranged from 98.86 to 101.03%. The intermediate precision were 0.42% for felodipine and was 1.01% for the related substances (n = 9). Limit of detection and quantification for felodipine and its related substances were 1 and 4ng, respectively. The solution remains stable within eight hours at room temperature. Finally, the method was demonstrated to be robust, resistant to small variations of chromatographic variables. This method is fast, simple, can be used for...
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Posted by admin on Sep 1, 2013 in |
In the present work in mathematical modeling, quantitative structure activity relationship (QSAR) studies were performed on some quinolone derivatives as a potential antibacterial agent using statistical work. The regression analysis of the data has shown that the activities of the compound can be modeled excellently in bi-parametric model.A heurisimatedtic algorithm selects the best multiple linear regression(MLR) equation showed the correlation between the observed values and the estimated values of activity is very good(R=0.9849, R2 =O.9700, PRESS=0.0185, 14Rcv2″> =0.9692, SPRESS =7.8631). The results are discussed...
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Posted by admin on Sep 1, 2013 in |
The past decade has witnessed intense interest in nutraceuticals in which phytochemical constituents have long-term health promoting or medicinal qualities. Secondary products have a variety of functions in plants; it is likely that their ecological function have some potential medicinal effects for humans. Between 1990 and 2020, deaths from non-communicable diseases and injury are expected to rise from 33 million to 58 million, with a similar proportional increase in mortality. It’s been estimated that by 2020, chronic diseases will account for almost three-fourths of all deaths. World Health Organization encourages the addition of secondary metabolites of plants in life saving drugs. Disorders like hypertension, diabetes mellitus, cardio vascular diseases, stroke, cancer, kidney disease and other diseases of unknown etiology are increasing due to poor diet pattern, stress, loss of physical activity, toxic substance in foods etc. Nutraceuticals and phytomedicines in the plant help in preventing these metabolic diseases without any side effects. Identification of these phytomedicines and Nutraceuticals and regular incorporation into the diet helps to increase the longevity...
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