Posted by admin on Nov 1, 2013 in |
The present work is regarding formulation, development and evaluation of losartan potassium microspheres using natural polymer. The mucoadhesive microspheres of losartan potassium were successfully developed by W/O emulsion solvent evaporation technique using two different natural polymers katira gum and babul gum. Total 12 batches were formulated. Six formulations were prepared by using each natural polymer i.e. LKM1 to LKM6 using katira gum (KG) and LBM1 to LBM6 using babul Gum (BG). All the formulations were evaluated for micromeritic properties, physical evaluation, which includes particle size analysis, percentage yield, drug content, drug entrapment efficacy, percent moisture loss and swelling index, in vitro dissolution studies, in vitro mucoadhesion, scanning electron microscopy, in vitro mucoadhesion and drug polymer interaction studies. The Optimized batch LKM5 was found to release the drug for 12 h (99.78%) and follows Higuchi Matrix model in dissolution studies, indicating the matrix-forming potential of natural polymer and diffusion controlled release...
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Posted by admin on Nov 1, 2013 in |
Partial purification and bioremediation of waste water by lipase from the marine actinomycete Streptomyces variabilis NGP 3 (Accession no: (JX843530)) were carried out. The optimum incubation period, pH, temperature and agitation speed for enzyme production were fifth day (61.2 U/ml), 9.0 – 9.5 (105 U/ml), 35ºC (39.4 U/ml) and 120 rpm (38.7 U/ml) respectively. Lactose (2.0 g/l) and peptone (0.6 and 0.8 g/l) proved to the best carbon and nitrogen sources respectively for lipase production. The partially purified lipase showed a specific activity of 1440.97 U/mg protein, 7.63 fold pure and yielded 3.19 per cent of protein. The enzyme activity was maximum at the pH and temperatures were 8.5 and 45ºC respectively. The molecular weight of the first and second isoenzymes was found to be 55.0 and 56.0 KDa respectively. Bioremediation of automobile effluent and slaughter house waste water were carried out by the isolated actinomycetes isolate S. variabilis NGP 3. The chemical oxygen demand (COD), total organic chloride (TOC) and fat/oil content of the effluent were analyzed. The...
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Posted by admin on Nov 1, 2013 in |
From this current research, Syk (spleen tyrosine kinase) protein and gene information is analyzed by different genomics, proteomics tools & databases. One crystal ligand 4DFL was collected from protein data bank (pdb). From different literature review 131 syk protein inhibitors were collected. Molecular modeling of these 131 molecules was done through Accelrys discovery studio (ADS). Choose appropriate force-field & minimization (Smart, Stephent Descent, and Conjugate Gradient) according to selected molecules. Then collected crystal ligand is purified by protein purification method and used appropriate conformation (BEST, FAST, and CAESAR). Docking methods were analyzed with protein, crystal ligand and similar inhibitors to know the best protein-ligand interaction. Pharmacophore research is done through HIPHOP and HYPOGEN method. Protein with final compound docking method is done after completion of virtual screening method. Pharmacophore research with final molecule was done. Quantitative structure activity relationship (qsar) method is analyzed to know the correlation between the above selective structures. From virtual screening method, best and final compound is analyzed. So, final molecule can be a drug...
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Posted by admin on Nov 1, 2013 in |
A simple, accurate, sensitive and reproducible UV spectrophotometric method has been developed for the determination of Tulobuterol (TLB) in bulk and also in its pharmaceutical dosage formulations. The proposed method showed absorbance maxima at 212 nm. Beer’s law is obeyed over a concentration range of 25-125µg/mL. The respective linear regression equation being Y =0.009x +0.014 for TLB. Results of analysis for the method established, was validated statistically and also by recovery studies. The apparent molar absorptivity and Sandell’s Sensitivity values are 0.43×104 Lmol-1cm-1 and 0.0371 µgcm-2 respectively. The assay and recovery studies were found to be 99.16% and coefficient correlation(r) was found to be 0.999. The different experimental parameters effecting the development and stability were studied carefully and optimized. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were also obtained. The developed method employed was successful for the determination of TLB in various pharmaceutical...
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Posted by admin on Nov 1, 2013 in |
Thyme (Thymus vulgaris L.) is a pleasant smelling perennial shrub, which grows in several regions in the world. The plant is reported to have high economic and medicinal values. The objectives of this research was to determine antimicrobial activity of petroleum ether, chloroform and hydroalcoholic extracts obtained from leaves of Thymus vulgaris collected from Oman. The other objective was to determine cytotoxic activity of the three extracts. Antimicrobial activity was measured using disc diffusion method against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia. Brine shrimp test was used to estimate cytotoxic activity. In antibacterial assay, Thymus vulgaris leaves extracts showed very strong results, inhibition zones ranged from 7 – 20 mm. So this plant can be used as a good source of potential antimicrobial agent.Petroleum ether showed the highest antibacterial activity. Furthermore, Petroleum ether and Chloroform extracts have almost killed all the shrimp larvae at higher concentration of 1000 mcg/ml. LC50 values for the two extracts were 85.2 and 95.8 mcg/ml, respectively. Polar fractions like hydro alcoholic...
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