Posted by admin on Aug 1, 2013 in |
Antibiotic is one of the most important commercially exploited secondary metabolites produced by bacteria, fungi and Streptomyces and employed in a wide range. Most of the antibiotics used today are from the microbes. Bacteria are easy to isolate, culture, maintain and to improve their strain. Bacillus species being the predominant soil bacteria because of their resistant endospore formation and production of vital antibiotic like polymyxin, bacitracin etc. are always found inhibiting the growth of the other organisms. In the present research study, screening of bacteria, fungi and Streptomyces with potential antibiotic activity was carried out. Among the microbes isolated and identified, Bacillus subtilis, Penicillium chrysogenum and Streptomyces spwere selected on the basis of their anti-bacterial activity. The inhibitory activities of the isolated microorganisms were checked against Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Klebsiella pneumonia (ATCC 4352). It was observed that Penicillium chrysogenum metabolites showed maximum antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumonia with a zone of inhibition...
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Posted by admin on Aug 1, 2013 in |
Walnuts from Northwest region of Syria was collected to determine Omega-6 (linoleic acid (LA, 18:2n-6)) and Omega-3 (α-linolenic acid (ALA, 18:3n-3)) in their oils, also called essential fatty acids (EFAs). This work was carried out using two different portions of samples. First samples were collected at harvest and second samples stored for one year under normal conditions. The extraction of the oil contained in Walnuts was done with hexane by ultrasonic technology, extract contained fatty acid and these fatty acids were estered. Fatty acid methyl esters were analyzed by gas chromatography with flame ionization detection (GC-FID). Syrian Walnuts major fatty acids were 18:2n-2 (linoleic acid) and 18:3n-3 (α -linolenic acid). Regarding n-6/n-3 ratio corresponded to the desired profile; Syrian Walnuts could be of potential interest due to their high nutritive value and lipid...
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Posted by admin on Aug 1, 2013 in |
The main objective of present work was to formulate and evaluate swellable controlled porosity osmotic tablets of Acyclovir for the treatment of herpes simplex. This formulation aims to release the drug in zero order pattern, increase bioavailability, reduce frequency of drug dosing and hence increased patient compliance. Acyclovir is a synthetic purine nucleoside analogue that is specially activated by Herpes Simplex Virus (HSV) induced thymidine kinase, and inhibits viral DNA polymerases as well as acting as a chain terminator. The technique used for the preparation of tablets was direct compression followed by deep coating of core tablets and total nine formulations (F1-F9) were prepared. Prior to compression, the prepared granules were evaluated for flow and compression characteristics. Prepared osmotic drug delivery system was also evaluated for in vitro drug release study. In vitro release profile of all formulations was in range from 54.12-99.85%. The formulation F2 was best amongst all and showed 56.58% drug release in 12 hr and 98.92% in 24 hrs. Zero order drug release kinetics was...
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Posted by admin on Aug 1, 2013 in |
Topoisomerase enzymes are highly expressed in cells which undergo rapid multiplication. Inhibition of this enzyme represents a potential therapeutic approach for diseases such as cancer. In order to understand the structure activity correlation of 2, 4, 6 pyridine based topoisomerase inhibitor, we have carried out a combined pharmacophore modelling, 3D-QSAR studies, molecular docking and virtual screening studies. A five point pharmacophore with hydrogen bond acceptor (A), hydrogen bond donor (D) and three aromatic rings (R 5, R 6, R 7) was used to derive a predictive atom based 3d-qsar model. The generated model had showed good correlation coefficient for training set and test set (R2=0.91and Q2=0.827). It was also validated using enrichment factor (EF) and goodness of hit score (GH score) and was used for virtual screening of compounds from ‘zinc drug like database’. Docking study of the hits retrieved from virtual screening revealed the binding affinity of these inhibitors at the active site of topoisomerase enzyme. In silico ADME predictions was also performed. These findings provide a set...
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Posted by admin on Aug 1, 2013 in |
Aim: The present study investigates the effect of Ramipril on blood sugar level and interaction with the oral anti-diabetic drugs in alloxan induced diabetic rats. Method: Rats were divided into 10 groups (n = 6) where Group I –II were normal and Group III –X were diabetic. Ramipril at the dose of 0.9 mg/kg body weight were administered to the normal and diabetic rats. In diabetic groups, the Ramipril was given concomitantly with the each of three Oral anti-diabetic drugs to find-out the effect on blood sugar level .All drugs administered orally once a day for 13 days and at the end of the experimentation Oral Glucose Tolerance Test (OGTT) was conducted. Results: It was observed that in normal rats the Ramipril slightly reduced the blood sugar level which was insignificant (P>0.05) except at ‘30’ min time point. In contrast in, it exhibited the anti-hyperglycemic activity significantly (P≤0.01) at all-time points in alloxan induced diabetic rats. Conclusion: The present study has concluded that The Ramipril has significant anti-hyperglycemic activities...
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