Posted by admin on Oct 1, 2013 in |
A simple, rapid and validated high performance liquid chromatography (HPLC) method with UV detection for the quantification of atorvastatin Calcium microemulsions (ATC-MEs) in rat plasma was developed. The assay procedure involved chromatographic separation using a C-18 chromatographic column (250 mm × 4.6 mm, i.d., 5 μm) and a mobile phase of methanol/water (containing 0.05 % glacial acetic acid) (70:30, v/v) 1.0 mL/min and detection wavelength of 248 nm. Plasma sample (200 μL) pretreatment was based on simple deproteinization by ethanol and centrifugation. The organic layer was evaporated under N2 gas, reconstituted with 200 μL of mobile phase, and 20 μL portions of reconstituted sample were injected onto the column. Calibration curve was linear (r ≥ 0.9854) from 0.1 to 50 mg/L. Both intra- and inter-day assay precisions that are presented through RSD were lower than 12.4% for intra-day and lower than 12.5% for inter-day assessment. This method was omitting the use of expensive solid phase extraction and time consuming liquid extraction procedures. Moreover, the present method was successfully applied...
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Posted by admin on Sep 1, 2013 in |
Emesis is a clinical condition which requires immediate treatment. The present work is aimed to formulate Orodispersible Tablet (ODT) of BCS class II drug Domperidone using Kyron T-314 as superdisintegrant and Avicel 102 as disintegrant by direct compression method. The drug-excipients compatibility study was carried out by Fourier Transform Infrared (FTIR) and Differential Scanning Calorimetry (DSC) which reveals no interaction between drug and excipients. A 32 factorial design was used to investigate effect of independent variables viz. superdisintegrant (Kyron T-314) and disintegrant (Avicel 102) on dependent responses like friability, disintegration time and percent drug release. Sodium lauryl sulphate (SLS) was used in the formulation to aid the dissolution of drug. All the formulations were evaluated for hardness, friability, disintegration time and dissolution rate. The factorial batch F4 containing 1.5 mg Kyron T-314 and 5 mg Avicel 102 has shown better results for all evaluation parameters with drug release of 99.22% and disintegration time of 29 sec. Therefore, factorial batch F4 was selected as the optimized...
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Posted by admin on Sep 1, 2013 in |
Kamrup District of Asasm is bounded by Udalguri and Baksa districts in the north, Meghalaya in the south, Darrang and Kamrup Metropolitan in the east and Goalpara and Nalbari district in the west. The rural part of kamrup district is mostly inhabitated by different tribes such as Boro, Khoronia Kochari, Rajbonghi, and Nepalis. An ethno botanical survey was conducted during the year 2011-2012 in some selected villages to explore the medicinal plants used by the local traditional healers known as ‘bej’ and ‘Ohjha’ for treatment of diabetes mellitus. The data’s were collected using questionnaire and personal interviews with few local healers. The investigation revealed 56 species of plants belonging to 38 families which are used to cure diabetes. These traditional ethno medicinal plants are consumed either in form of juice, powder, or boiled extract of leave, stem, root, seed, fruit, bark, rhizome and flower. In some cases it was found that the whole plant is directly used as potent...
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Posted by admin on Sep 1, 2013 in |
The present study was undertaken to know about the free radical scavenging activity of plants located near Thermal power plant, Mettur, Salem. In this study, Polyalthia longifolia, Derris indica, Holopetela integrifolia, Tamarindus indica, Terminalia tomentosa, Psidium guajava, Nerium indicum, Azadirachta indica, Calotropis gigantea, Ricinus communis, Carica papaya, Ficus benghalensis, Embilica officinalis, Tectona grandis, Ficus religosa, Mangifera indica, Moringa oleifera, Acacia Arabica, Opuntia ficus indica are the plants that were collected during March to April 2013. Among the plants selected, Holopetela integrifolia, Mangifera indica, Tamarindus indica, Acacia arabica, Azadirachta indica were found to have high flavonoid content. Total antioxidant activity was measured in terms of phospho-molybdenum activity was high in Carica papaya when compared to the other entire antioxidant assay, Acacia arabica showed maximum ability to chelate metal...
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Posted by admin on Sep 1, 2013 in |
Bromocriptine mesylate quick release formulation has recently been approved by FDA for the treatment of diabetes mellitus. Till now it has been mainly known for its use in Parkinsonism and for suppression of lactation. Present study evaluates and compares the antihyperglycemic effect of the combination of bromocriptine and metformin with either of the two drugs taken alone. For this fasting blood sugar level of all the groups were measured on day 1,7,14 and 21 of treatment. In this study all the three treatment groups was found to have significant (P<0.05) blood glucose lowering effect. Fall in fasting blood was less in bromocriptine treated group as compared to groups treated with metformin and those treated with combination of the two. It is concluded that hypoglycemic effect of combination of metformin and bromocriptine is better than either of the two drugs taken alone. This may be due to potentiation or synergism between metformin and bromocriptine. Further studies are required to prove that bromocriptine has constantly significant hypoglycemic effect with better safety...
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