Posted by admin on Aug 1, 2013 in |
A rapid, simple, sensitive and reproducible RP-HPLC method was developed for the determination of conessine (bioactive steroidal alkaloid) in Holarrhena pubescens, a highly valued medicinal plant extensively used in pharmaceuticals. Separation was achieved on Discovery (R) RP Amide C16 Supelco column (250 µm x 4.6 µm ID) using a mobile phase of acetonitrile: water (95:5 v/v) with flow rate 0.6 ml / min detection at λ 210 nm. Assessments of six extraction techniques were also performed to evaluate the conessine content and further the efficiency of the extracts against bacterial strains was tested to address causative relationship between the both. The method was linear over a concentration range of 2.5-20 µg/ml (r2= 0.9991). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at the three concentrations were higher than 98.8% with RSD<2%. Extract obtained from ultra-sonication method possessed highest conessine content (1.0647%) and inhibited most of the bacteria. The study confirms ultrasonic extraction to be an ideal method to obtain conessine enriched extract possessing...
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Posted by admin on Aug 1, 2013 in |
Medicinal plants have been reported to play an important role in modulating glycemic responses. Several mechanisms have been proposed for the anti-diabetic effect of medicinal plants such as inhibition of carbohydrate metabolizing enzymes, manipulation of glucose transporters, β- cell regeneration and enhancing insulin releasing activity. In the present study, the hypoglycemic potential of Artocarpus altilis leaves (AL), bark (AB) and fruit (AF) parts were investigated using suitable in-vitro and ex-vivo techniques such as glucose adsorption, glucose diffusion retardation index (GDRI), inhibition of enteric enzymes à α- amylase, α- glucosidase, sucrase and effect of selected samples on glucose uptake using yeast cell as model system. The cold and hot aqueous extracts of AB significantly (P ≤ 0.05) inhibited the activity of all the three enzymes followed by AL and AF, while ALP and ABP enhanced the activity of α- glucosidase. ALP enhanced the glucose uptake by yeast cells significantly (P ≤ 0.05) followed by ABP and AFP, which was dependent on glucose concentration.The results suggest that the possible mode of...
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Posted by admin on Aug 1, 2013 in |
Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well as reduce uremia, acute nephritis & nephritic syndrome. The present study was to evaluate the antiuremic and antioxidative effect of Asparagus racemosus (AR) on acetaminophen induced uremic male rats. The study was designed with 36 male albino rats which were randomly divided into 6 groups. Group I animals were provided normal food and water ad libitum, Group II, III, IV, V and VI received acetaminophen intraperitoneally at the dose of 500 mg/kg body weight/day for 10 days. Methanol, aqueous, hydromethanol, and hexane extract of AR at the dose of 500mg/kg body weight/day were fed orally on the 11th day and continue for next 15 days to group III, IV, V and VI respectively. After 25 days, group-II animals showed significantly increased (p<0.05) plasma urea, creatinine, sodium. Elevation of lipid peroxidation was noted by measuring Malondialdehyde level in both plasma and kidney tissues than Group I, III, and V. Plasma Potassium, plasma and...
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Posted by admin on Aug 1, 2013 in |
Nicorandil is occasionally given for cardiovascular disorders in patients also suffering from diabetes mellitus. Simultaneous use of nicorandil and sulfonylureas may interfere with the euglycemia. The study was done to find the effect of nicorandil on glucose tolerance in normal male Wistar albino rats, under oral glucose load, adrenaline injection and in receiving oral hypoglycemic drugs. Male albino rats 100-120g was divided into control and treatment groups. The control group of rats received either oral 2% gum acacia or normal saline subcutaneously. The treated groups received either glucose (1g/100g), glipizide (0.045mg/100g) or nicorandil (0.18mg/100g) orally alone or in combination. Blood samples were collected from lateral tail vein at 0, 1, 2 and 4hrs interval. Blood glucose estimation revealed that nicorandil significantly reduced glucose tolerance in normal rats and in rats made hyperglycemic by oral glucose load or adrenaline injection. It antagonized the hypoglycemic effect of glipizide and reduces glucose tolerance in rats at human therapeutic...
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Posted by admin on Aug 1, 2013 in |
Esomeprazole is used to treat gastro esophageal reflux disease. Naproxen is a Non-steroidal anti-inflammatory drug (NSAID) used in the treatment of pain or inflammation caused by conditions such as arthritis, ankylosing spondylitis, tendinitis, bursitis, gout, or menstrual cramps. A simple, precise, cost effective RP-HPLC method was developed and validated for the determination of both Esomeprazole and Naproxen in Pharmaceutical compositions. The chromatographic separation was achieved on a Symmetry C18 (4.6 x 150mm, 5mm, Make: XTerra) using a mobile phase consist of a mixture of phosphate buffer (pH 3) and Acetonitrile [60: 40]. The flow rate of mobile phase was maintained 1.0 mL per minute. The wavelength chosen for detection was 285 nm. The retention times of Esomeprazole and Naproxen peaks were around 2.105 and 3.555 mins respectively. The Accuracy was calculated for 50%, 100% and 150% and the % recovery was found to be 98.0%-100.4%. The method was found to be linear over the range of 5ppm to 9ppm per mL for Esomeprazole 125ppm to 225ppm per mL for...
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