Posted by admin on Jul 1, 2013 in |
A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Pregabalin in pharmaceutical Tablet dosage form. The mobile phase consisted of 50:50 % (v/v) of Methanol & 10mM Ammonium Acetate (pH adjusted to 3.0 with glacial acetic acid) operated on isocratic mode. The flow rate is 0.7 ml/min. Chromatographic determination of Pregabalin was performed on Phenomenex C18 column (150 X 4.6 mm Id, ODS 2, 5µm). The wavelength of detection is 210 nm. The injection volume is 20µL. The retention time of Pregabalin is 3.39 ± 0.10 minutes. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, solution stability, ruggedness, and robustness. The influence of Acid, Alkaline, Oxidative Stress, Photolytic stress conditions on Pregabalin was studied. Results indicated that pregabalin is stable under the experimental conditions with a high baseline noise observed in alkaline medium. The proposed method has been successfully used for the estimation in tablet dosage...
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Posted by admin on Jul 1, 2013 in |
The ethanol extract of Solanum surratense(EESS)(Family: Solanaceae) root was investigated for its anti-diabetic effect in Wistar Albino rats. Diabetes was induced in Albino rats by administration of single dose of Alloxan monohydrate (150mg/kg). The ethanol extract of Solanum surratense at the dose of 200mg/kg and 400mg/kg, p.o. was administered as single dose per day to diabetes induced rats for a period of 21 days. The effect of EESS root on body weight of the animals, blood glucose, serum lipid profile [cholesterol, triglycerides], serum enzymes[serum glutamate oxaloacetate transamines(SGOT), Serum glutamate pyruvate transaminases(SGPT), alkaline phosphatase(ALP)], total protein were measured in the diabetic rats. EESS root elicited significant (p< 0.01) reductions of blood glucose, lipid parameters and serum enzymes and significant (p<0.01) reductions of blood glucose. From the above result, it is concluded that EESS root possesses significant anti-diabetic effects in Alloxan induced diabetic rats....
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Posted by admin on Jul 1, 2013 in |
Medicinal plants are a source of naturally active compounds used extensively by tribal people worldwide for many ailments. Withania somnifera (WS) is one such plant used to treat many ailments from the time of Ayurveda. Extraction of the bioactive plant constituents from the whole plant or from the different parts of the plant has always been a challenging task. As the dried roots of WSare widely used in the treatment of many disorders, the current investigation aimed at extraction and detection or screening of active phytochemical compounds from different extracts of WS root. Phytochemical screening of different extractions revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, steroids, terpenoids, glycosides and reducing sugars which could account for its varied medicinal properties like anti-inflammatory, anti-spasmodic, anti-analgesic, neuroprotective and diurectic...
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Posted by admin on Jul 1, 2013 in |
Gum Dammar is a natural resinous material of plant Shorea javanica, family–Dipterocarpaceae. In the present investigation Dammar gum (DG) and alcohol soluble fraction of Dammar gum (DA) are evaluated for their physicochemical, mechanical and film forming characteristics. Films were produced by casting/solvent evaporation method from plasticizer free and plasticizer containing solutions. Films prepared from different formulations were studied for their mechanical (tensile strength, percent elongation and Young’s modulus), water vapor transmission and moisture absorption characteristics. Unplasticized DG, DA films were brittle and posed the problem of breaking during handling. Hydrophobic plasticizers, dibutyl phthalate (DBP), improved the mechanical properties of free films with plasticizer showing significant effects on film elongation. Thus present study proposes DG and DA as excellent film...
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Posted by admin on Jul 1, 2013 in |
The homo and hetero interaction in the binary mixtures of Ethyl acetate + n-Butylamine, Ethyl acetate + Cyclohexylamine and Ethyl acetate + Diethylamine has been investigated through the orientation of dipoles using dielectric polarization method. The dielectric and thermodynamic parameters such as Kirkwood correlation factor, Excess permittivity, Bruggemann factor and Dipolar excess free energy for the above systems have been calculated from the experimentally measured values of capacitance and refractive index of solutions, at temperatures 303K, 313K and 323K.In all the studied systems, the dielectric parameters obtained show systematic change in the molecular structure with the concentration of amine in the solution. It indicates that the β-multimer formation due to the presence of hetero interaction between the components of above...
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