Posted by admin on Apr 1, 2013 in |
General phytochemical screening of the aqueous extract of fruits of Solanum xanthocarpum (Solanaceae) revealed the presence of steroids, terpenes, phenolic compounds, saponins, fatty acids, alkaloids. Fractionation of aqueous extract and activity guided isolation resulted in isolation of two white crystalline powder which were subjected to physical, chemical and spectral identification using IR, 1H‐NMR, 13C‐NMR and LC‐MS. On the basis of the spectral data analysis and chemical reactions, the compounds have been established as β-sitosterol and Stigmasterol....
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Posted by admin on Apr 1, 2013 in |
ABSTRACT: Fifteen soil samples were collected from different locations near Nagpur i.e. from Nagpur, Bhandara and Chandrapur. Air drying and pre-treatment of soil samples with CaCo3 (1%) were found to be most effective for isolation of actinomycetes. Five different selective media were used for the isolation of actinomycetes and the best growth was observed in yeast malt extract agar medium with 1 mg/ml cycloheximide as antifungal agent and by using double layer agar technique for isolation. Significant differences were found between the counts of actinomycetes on different media. Total 78 actinomycetes species were isolated. More number of actinomycetes were isolated from Nagpur region (32 x 10 cfu/g) as compared to Bhandara (28 x 10 cfu/g) and Chandrapur (18 x 10 cfu/g) regions. Out of 78 isolates 23 isolates showed positive results in primary screening. Maximum isolates showed activity against B. subtilis, B. cereus, S. aureus and E. coli. Few isolates showed activity against P. vulgaris, P. aeruginosa, C. albicans and A. niger. Noactivity was seen against Penicillium sp. From...
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Posted by admin on Apr 1, 2013 in |
The herbal preparation of the leaves of Solanum pubescens Pers (Solanaceae) is used in traditional medicine for the treatment of Epilepsy and febrile convulsions. A pilot study has confirmed the potency of the leaf of Solanum pubescens in the control of seizure in mice. The current study was aimed to identify the active phytochemical responsible for the activity. Results indicated that methanolic extract of Solanum pubescens at 300 mg/kg exhibited a significant P<0.05 delay in the latency of myoclonic spasms and tonic-clonic phase of seizure induced by PTZ, and also decreased the latency and increased the duration of phenobarbitone induced sleeping time. While the phytochemical studies showed the presence of alkaloids, resins, glycosides, carbohydrates, reducing sugar, fats and oils, flavonoids and terpenoids. The results suggested that the extract possessed anticonvulsant activity and central depressant effects which may be attributable to the...
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Posted by admin on Apr 1, 2013 in |
An UV spectrophotometric method for the quantitative determination of Temozolomide (TMZ) in bulk and capsule was developed in present work. The parameters linearity, precision, accuracy, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. UV spectroscopic determination was carried out at an absorption maximum of 328 nm using 0.1N Hydrochloric acid as solvent. In the UV spectroscopic method linearity over the concentration range of TMZ was found to be 2-18 µg/ml with a correlation coefficient 0.999. The limit of detection and limit of quantification were found to be 0.5271 and 1.6454 mg/ml respectively. Results of the analysis were validated statistically and by recovery studies. The proposed method is simple, rapid, precise, accurate and reliable and can be used for the routine quantitative analysis of TMZ in bulk and pharmaceutical...
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Posted by admin on Apr 1, 2013 in |
Plants of medicinal importance though are quite well known among the Ayurvedic practitioners since years, yet many of them have not been standardized, validated and documented completely. Among such medicinally valuable plants, Salacia holds a place as an effective antidiabetic herb and many species of this plant are in use as anti-diabetics. The present study reports the phytochemical analysis of one of its species Salacia oblonga (SO) (roots and stems), belonging to the family Celastraceae (bittersweet), through various biochemical and chromatographic methods. TLC and HPTLC analysis of six different extracts of SO revealed numerous bands, indicating the presence of diverse groups of phytocompounds, many of them are assumed to contribute significantly to its antioxidant activity as well as, other biological activities. This type of analysis can help in fingerprint profiling of the plant and its various species. The identification and characterization of the phytocompounds can further help in finding out molecular targets/mechanism of action of the constituents of this herb that are responsible for its biological...
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