Posted by admin on Jun 1, 2013 in |
A simple, sensitive, selective, precise and stability indicating high-performance thin-layer chromatographic method for determination of quetiapine fumarate both as a bulk drug and from tablets was developed and validated as per the International Conference on Harmonization (ICH) guidelines. The HPTLC method employed aluminum plates precoated with silica gel G 60F254 as stationary phase. The solvent system consisted of methanol: butanol: ethyl acetate (2:1:1) and give compact spots for quetiapine fumarate with Rf value 0.76 ± 0.01 cm. Densitometry analysis was carried out in the absorbance mode at 220 nm. Linear regression analysis showed good linearity (r2 = 0.999) with respect to peak area in the concentration range of 1000–5000 ng/spot. The method was validated for precision, accuracy, specificity and robustness. The limits of detection and quantitation were 171.65 and 520.18 ng/spot, respectively. The method was validated for precision, accuracy, ruggedness and recovery. Quetiapine fumarate was subjected to acid and alkali hydrolysis, oxidation, thermal and photodegradation. The degraded products were well separated from the pure drug. The statistical analysis proves...
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Posted by admin on Jun 1, 2013 in |
A reversed-phase-liquid chromatographic (RP-HPLC) method was developed for the determination of Paracetamol (PCM) and Dicyclomine Hydrochloride (DICY) in their bulk drug and marketed formulations. A reversed-phase C-18 Phenomenex C18 column (250 mm × 4.6 mm i.d., 5μm) with mobile phase consisting of Acetonitrile: Phosphate Buffer pH (5.5): triethylamine (75:25:0.02 v/v/v) was used with 1.0 ml/ min flow rate and detected at 218 nm. The retention times of Paracetamol and Dicyclomine Hydrochloride were found to be 2.65±0.3 min and 11.32±0.3 min, respectively. Developed methods were validated according to ICH guidelines. Linearity was observed at concentration range of 100-500 µg/ml for Dicyclomine Hydrochloride and 10-50 µg/ml for Paracetamol followed by Beer’s law. The % recoveries of Paracetamol and Dicyclomine Hydrochloride were found to be between 101.05-101.20% and 100.55-100.95%. The percentage RSD for the method precision was found to be less than 2%. The proposed method is precise, accurate, selective and rapid for simultaneous determination of Dicyclomine Hydrochloride and...
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Posted by admin on Jun 1, 2013 in |
Ni(II) and Cu(II) complexes of Schiff base thiosemicarbazones (L1) and semicarbazones (L2) derived from pyrrole-2-carboxaldehyde. These complexes are characterized by elemental analysis, molar conductance measurements, magnetic suscepectibility measurements, mass, IR, electronic and EPR spectral studies. The molar conductance measurements of the complexes in DMSO determine the non-electrolytes and electrolytes nature of the complexes based on their molar conductance value as higher value indicates the electrolytes nature of the complexes and lower value of the molar conductance indicates the non-electrolytes nature of the complexes. On the basis of spectral studies an octahedral geometry may be assigned for Ni(II) complexes except [Ni(L1)2](NO3)2 which are of tetrahedral geometry. A tetragonal geometry may be suggested for Cu(II)...
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Posted by admin on Jun 1, 2013 in |
Background: Glioblastoma Multiforme is the commonest of all astrocytomas. It accounts for 12-15% of all intracranial neoplasm and 50-60% of all astrocytic tumors. Most common site of occurrence is cerebrum and age incidence is highest in fifth and sixth decade. Gliosarcoma constitutes 2% of all brain tumors, 5% of all astrocytomas and 8% of anaplastic astrocytomas. The cell of origin of sarcomatous component has not been fully established. Aim: The purpose of our study is to evaluate histopathological features of GBM in detail, in relation to the age, sex and site of occurrence, in Ganga Ram Hospital, during a period of 10 years and also to find out the relative frequency of Gliosarcoma among glial tumors. Particular emphasis is on to evaluate the nature of glial as well as mesenchymal component and their relationship in Gliosarcoma with the help of immunohistochemistry. Materials: Total of 115 cases of Glioblastoma multiforme were studied histopathologically in detail, with some clinical corelation in Sir Ganga Ram hospital. 13 cases of Gliosarcomas, diagnosed morphologically...
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Posted by admin on Jun 1, 2013 in |
The usefulness of computational methods in drug design process is no longer a point of debate. The contributions from this evolving field have become apparent to both pharmaceutical scientists and academic biomedical researchers. As all the computational drug design are based on statistical and mathematical calculations there is strong evidences present for each and every step, thus research by chance is no longer exist. From statistical analysis of large data set, up to quantitative prediction, one can get simply by seating in front of graphical terminal and using this technique. Success stories from these endeavors are appearing in the literature at an impressive rate. Since the search for new antiviral drugs with reduced toxicity and lower side-effect is continues. Antiviral of class HIV reverse transcriptase are found to have wide range of activities but still this drugs are not very effective in curing HIV infected persons. So this drugs study is carried out and will show more biological and therapeutic activity by performing work of homology modeling and docking...
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