Posted by admin on Mar 1, 2013 in |
The aim of this research is to observe the cytotoxic activities of methanolic crude extracts of Ocimum sanctum (Family: Lamiaceae). The leaves of Ocimum sanctum was extracted with organic solvent (methanol) and the extracts were fractionated by using solvent-solvent partition. The n-hexane, ethyl acetate, and chloroform soluble fractions of methanolic crude extract of Ocimum sanctum were screened for cytotoxic activity using brine shrimp lethality bioassay. A reputed cytotoxic agent, vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it was well predicted that n-hexane, ethyl acetate, and chloroform soluble fractions of methanolic crude extracts possess cytotoxic principles (LC50 4.36 mg/mL, LC50 5.37 mg/mL and LC50 10.00 mg/mL respectively) comparison with positive control, vincristine sulphate (LC50 0.563...
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Posted by admin on Mar 1, 2013 in |
The present investigation is mainly on the synthesis, characterization and anti-microbial screening of certain new curcuminoid analogues containing imidazole, pyrrole and thiophene rings and their metal complexes. The ability of such heterocyclic β-dicarbonyl compounds and their metal ions to influence many of complex reaction upon which the vital processes of micro-organisms depends is the motivation behind the work. A series of 5- hetero aryl-1-phenyl-4-pentene-1,3-diones(1a-c) and their Cu (II), Ni (II) complexes of ML2 stoichiometry were synthesized and characterized by UV, IR, mass and 1H NMR spectroscopies. Analytical and spectral data suggest neutral bidentate coordination for unsaturated diketone with metals. Anti-microbial screening was carried out by using Kirby-Bauer disc plate method. All the ligands and their metal complexes showed significant anti-microbial action. Further complexation; seem to augment the antimicrobial activity of the...
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Posted by admin on Mar 1, 2013 in |
Tinospora crispa, a plant belonging to the family of Menispermaceae is a well-known traditional medicinal plant used in Bangladesh. The methanolic extracts of stems of the plantwere evaluated for their thrombolytic and preliminary cytotoxic activities. The thrombolytic activity was evaluated using the in vitro clot lysis model while while the screening of cytotoxic activity was done using brine shrimp lethality bioassay. Different concentrations of stem extract were made in isotonic saline (2.5, 5, 10 and 20 mg/ml) and added to pre-clotted human blood (n=13) and the clot lysis activity was determined following 90 minute incubation at 37º C. The results showed that T. crispa stem extract induced mild to moderate (14.81- 25.73%) and significant (p <0.001) clot lysis activity whereas the standard Streptokinase (30,000 I.U.) evoked a significant (p < 0.001) clot lysis activity (50.1%). In Brine shrimp lethality bioassay, the LC50 values of methanolic crude extract and its pet ether, chloroform and aqueous fractions were found to be 6.43, 0.8. 4.58 and 7.46 mg/ml respectively which were comparable...
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Posted by admin on Mar 1, 2013 in |
The present investigation highlighted the formulation and release characterization of Ketorolac Tromethamine loaded matrix tablet. Various formulations of tablets were prepared by direct compression method along with Kollidon SR and Hydroxy Propyl Methyl Cellulose (HPMC) as release retardant polymers. Each of the formulated tablets contains 50mg Ketorolac Tromethamine. The evaluation involved physical properties studies (weight variation, thickness, length, width, hardness, friability, and drug content) of tablets and in vitro release kinetics assessment. The USP paddle method was operated at 50 rpm selected to perform the dissolution test and 900 ml phosphate buffer of pH 7.4 was used as dissolution medium. The drug release from each formulation was analyzed by using release kinetics theories. All formulations followed Higuchi release kinetics. When the release data was plotted into Korsemeyer-Peppas equation, then it was confirmed that F-1 to F-5 exhibited fickian type drug release whereas F-6 exhibited non-Fickian type drug release. The in-vitro release studies revealed that the formulation F-4 can be taken as an ideal or optimized formulation of sustained release...
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Posted by admin on Mar 1, 2013 in |
A simple and sensitive UV spectrophotometric method has been developed for the quantitative estimation of trimetazidine in bulk drug and pharmaceutical dosage forms (tablets). Trimetazidine exhibited absorption maximum at 269 nm in 0.1 N HCl and obeyed Beer’s law in concentration range 5-25 µg/ml. The result of analysis in this method has been validated statistically and by recovery studies. This method is extended for the analysis of drug in pharmaceutical...
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