Posted by admin on Feb 1, 2013 in |
The present study was undertaken to evaluate and compare the antioxidant activity and brine shrimp lethality bioassay of different parts (leaf and bark) of the plant Acacia auriculiformis. DPPH radical scavenging and NO scavenging capacity were measured for the determination of antioxidant activity. Ethyl acetate fraction of bark was found to possess highest DPPH radical scavenging activity with IC50 value of 7.80 µg/ml followed by methanol extract of leaf (IC50 value of 7.95 µg/ml). The IC50 value of standard ascorbic acid was 33.77 µg/ml. Incase of NO scavenging activity, n-hexane fraction of root found to have highest scavenging activity with IC50 value of 1.75 µg/ml followed by ethyl acetate fraction of leaf (IC50 value of 3.35 µg/ml). The IC50 value of standard ascorbic acid was 71.06 µg/ml. When compared with other fractions, the methanol fraction of leaf and bark had highest cytotoxic activity with LC50 value of 0.55 and 0.79 µg/ml respectively. This was followed by ethyl acetate fraction of leaf with LC50 value of 0.95 µg/ml. The standard...
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Posted by admin on Feb 1, 2013 in |
Antimicrobial activity of extracts leaves of Murraya koenigii (Curry) and Camellia sinensis (Tea) plants prepared in water, methanol and ethanol by cold maceration method were evaluated for their antimicrobial activity against Staphylococcus aureus (3) strains, Pseudomonas aeruginosa (3) and Candida species: Candida albicans (2 and one strain each of Candida tropicalis, and Candida parapsilosis. The plant materials were procured from Kangra District of Himachal Pradesh, India. Methanol and ethanol extracts of both the plants produced significant zones of inhibition against all the bacterial and fungal pathogen used in the study in the antimicrobial assay. However, aqueous extract did not show any significant activity. In this assay, the susceptibility of Staphylococcus aureus to Gentamicin and Ampicillin, Pseudomonas species to Ciprofloxacin and Candida species to Amphoterecin B was also assessed for comparative analysis. All the Staphylococcus strains were sensitive to Gentamicin but resistant to Ampicillin, but Ciprofloxacin had significant inhibitory effect against Pseudomonas strains except one, PA-39. All the strains of Candida species were resistant to Amphoterecin B. Phytochemical screening of...
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Posted by admin on Feb 1, 2013 in |
In Siddha system of medicine, the two formulations Vedikara silasathu parpam and Nerunjil kudineer in combination is given for urinary tract infection. Inhibition in the synthesis of inflammatory mediators and prostaglandins owe to the anti- inflammatory and antinociceptive properties of the drugs. In this study, the two Siddha formulations VSP and NK in combination was evaluated for anti- inflammatory activity by means of carrageenan induced rat paw edema method and antinociceptive activity with tail flick method in Wistar albino rats. In the carrageenan induced paw edema technique, rats at the dosage of 500mg/kg/p.o of VSP and NK, significant (p<0.001) inhibition of inflammatory progression was observed than the control group. In the tail flick method, VSP and NK at dosage of 500 mg dose, increased the tail withdrawal time significantly (p<0.001) when compared to the control group. This study had established the significant anti- inflammatory and antinociceptive activity of VSP and...
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Posted by admin on Feb 1, 2013 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-diabetic drugs. The separation was achieved on ACE 3 150mm*4.6mm, 3.5µm column with gradient flow. The mobile phase at a flow rate of 1.5 mL min−1 consisted of 10mM sodium hexane sulphonate monohydrate and 10mM Potassium dihydrogen phosphate buffer with acetonitrile and methanol in gradient ratio. The UV detection was carried out at 210 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
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Posted by admin on Feb 1, 2013 in |
The aerial roots of Ficus bengalensis Linn. were collected in and around Chennai, Tamilnadu, India They were washed, shade dried and were ground into powder. The powder was extracted with chloroform, petroleum ether, methanol and hexane. The antibacterial screening of the extracts was carried out by determining the zone of inhibition using disc diffusion method. The strains were grown to logarithmic phase in BHI broth and the inoculum was prepared by adjusting the turbidity of bacterial suspension to 0.5 McFarland’s tube. The dried extracts was dissolved in 10% Dimethyl sulphoxide (DMSO) in required concentration. The sterile discs were impregnated with 20 μl of extract. The extract discs were placed on BHI agar plates, which were previously inoculated with test strains and incubated at 37oC for 24 hours. Ampicillin disc (10μg) and 10% DMSO impregnated discs were used as positive and negative controls respectively and the zones of inhibition were recorded. The Minimum inhibitory concentration was determined by agar dilution method. The results of the present study showed that the...
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