Posted by admin on Mar 1, 2013 in |
The different fractions of crude methanol extract of stem of Stephania japonica (Thunb.) Miers (Menispermaceae) was screened for its analgesic and antidiarrhoeal activities. The different fractions of crude extract produced significant writhing inhibition in acetic acid induced writhing in mice at the dose of 250 and 500 mg/kg body weight comparable to the standard drug diclofenac sodium at the dose of 25 mg/kg of body weight. When tested for its antidiarrhoeal effects on castor oil induced diarrhea in mice, it increased mean latent period and decreased the frequency of defecation significantly at the dose of 250 and 500 mg/kg body weight comparable to the standard drug loperamide at the dose of 50 mg/kg of body weight. The overall results suggest the analgesic and antidiarrhoeal activities of the different fractions of crude extract. Among all the fractions Ethyl acetate soluble fraction showed most significant analgesic activity and Petroleum ether soluble fraction showed the most significant antidiarrhoeal...
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Posted by admin on Mar 1, 2013 in |
A new, simple, precise, rapid, accurate and stability-indicating RP-HPLC method has been developed for the determination of Entacapone in presence of its degradation products or other pharmaceutical excipients. Stress studies were performed on Entacapone tablets and it was found that it degrades sufficiently in acidic, alkaline and oxidation conditions, while negligible degradation was observed in thermal, photolytic and humidity conditions. The peaks of the degradation products were not observed in the chromatogram due to the nonchromophoric nature of the degradation moiety formed. The separations were carried out on a Hypersil BDS C8, 150 mm x 4.6 mm 5µm particle size column with a mobile phase consisting of Acetonitrile: 0.01% Orthophosphoric acid pH 2.5 at a flow rate of 1.0 ml/min. Detection wavelength was 210nm. The retention time of Entacapone was 7.0minutes. The developed method was validated in terms of specificity, forced degradation, linearity, precision, intermediate precision (ruggedness), accuracy, range and robustness. The calibration curve for Entacapone was linear (correlation coefficient 0.99995) from range of 50µg/ml to 150µg/ml. Relative standard...
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Posted by admin on Mar 1, 2013 in |
The aerial parts essential oils of Ocimum basilicum (Lamiaceae) from Togo were steam-distilled and investigated for their percentage composition (GC and GC/MS) and in vitro antimicrobial activities. Five oil chemotypes were identified and classified as follows in line with their principal components: estragole type; linalool/estragole type; methyleugenol type; methyleugenol/t-anethole type; tanethole type. The in vitro microbiological experiments revealed that only the methyleugenol and methyleugenol/t-anethole chemotypes were active against tested fungi and bacteria. Their minimum inhibitory concentrations (MIC) ranged from 80-150 μL/L and from 200-500 μL/L respectively. Likewise, on tested bacteria the MIC varied from 200-400 μL/L and from 250-500 μL/L respectively. These findings are supportive of the potential of both basil oil chemotypes for use as active ingredients in natural antibiotic...
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Posted by admin on Mar 1, 2013 in |
The methanolic extract (MeOH) of the bark of Holoptelea integrifolia (Roxb.) Planch and its partitions namely n-hexane (Hx), ethyl acetate (EtOAC), butanol (BuOH) and water (H2O) was screened for various in vitro biological activities including general cytotoxicity (brine shrimp lethality assay), phytotoxicity (lemna minor bioassay), nematicidal and insecticidal (Tribolium castaneum H., Rhyzopertha dominica F. and Callosobruchus analis F. assays. The extracts were found to have potent significant nematicidal activity against Meloidogyne incognita and moderate insecticidal activity against C. analis F. At the tested concentrations non-significant cytotoxicity and phytotoxicity had intended us for the utilization of extracts as biological control for the agro-ecosystem management having prudent, effectual and eco-friendly...
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Posted by admin on Mar 1, 2013 in |
In the present study, total 15 fungal species isolated from roots of drug Stereospermum chelonoides DC. such as: Fusarium solani, F. lateritum, F. semitectum, F. oxysporum, Aspergillus ochraceus, A. niger, A. terreus, Theilavia terricola, Papulaspora immerse, Scytallidium thermophilum, Didymostilbe sp., Cunningamella elegans, Aphanomyces sp., Sordaria fimicola and Mucor praini. Species of Fusarium showed maximum percentage incidence. The root samples stored at different relative humidities 30, 50, 75, 96 and 100% RH. Quantitative estimation of alkaloids and glycosides in association with isolated fungi was done. Percentage incidence of storage fungi and the rate of deterioration of chemical constituents in the samples were noted at above 75% RH. Analysis of variance also showed the effect of relative humidity and incubation days on biodeterioration of these chemical constituents were significant at 5% level of...
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