Posted by admin on Jul 1, 2013 in |
Alstonia scholaris R. Br. (Fam: Apocynaceae) is widely used plant in Indian medicine for the treatment of various ailments. Among the complex constituents of this plant, ursolic acid, a triterpene acid is reported in the leaves of A. scholaris. Ursolic acid was isolated from the methanolic extracts of the leaves of A. scholaris. The isolated marker ursolic acid was authenticated by UV, IR, Mass and NMR spectroscopy. A simple sensitive and HPTLC method was developed for the quantitative analysis of ursolic acid in leaves of A. scholaris. The method was found to be precise, with relative standard deviation (RSD) values of intraday and interday analysis in the range of 1.28-3.41% and 2.43-3.07% respectively. Accuracy of the method was checked by conducting recovery study at three different levels (50, 100 and 125%) and the average recovery was found to be 100.57%. The content of ursolic acid was found to be 3.10 ± 0.01gms/100gms of powdered leaves of Alstonia scholaris. The proposed method is rapid, simple and accurate and can be...
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Posted by admin on Jul 1, 2013 in |
The term dementia refers to a severe loss of thinking abilities; especially memory. The most common kind of dementia is Alzheimer’s disease (AD). Bacopa monnieri and Withania somnifera are the most potent herbs for the treatment of loss of memory. Piper nigrum Linn. is essential ingredients of numerous prescriptions and formulations. It is reported that Black pepper increases the blood levels of drugs more than 100%. In this study, alcoholic extract of B. monnieri, methanolic extract of W. somnifera and powder of Piper nigrum and their combinations were subjected to young mice. Elevated plus Maze and Passive Avoidance apparatus served for the exteroceptive behavioural model for testing memory. Piracetam was taken as standard drug and Scopolamine was used to induce amnesia. In-vitro antioxidant activity was carried out by using DPPH free radical scavenging model and Gallic acid as standard drug. This study demonstrates that Alcoholic extract of Bacopa monnieri alone and Alcoholic extract of B. monnieri in combination with methanolic extract of Withania somnifera and powder of Piper nigrum...
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Posted by admin on Jul 1, 2013 in |
AIDS is syndrome of a set of disease, which is caused by HIV I and II. These viruses mainly destroy the lymphocytes which play a major role in our body immune system. Till now, there is not taken exactly prevention on this virus. Although some antiviral drugs are produced which make a preventive barrier out of the lymphocytes cells of patient’s blood but there is no way to kill the virus properly. The present work was carried out to find the prevalence of HIV in East Region of India. A total 717 patients were screened for the presence of HIV in their blood by various ELISA methods. It has been concluded that out of 717 patients 219 patients were infected with HIV and in between 15 to 45 ages, the infection rate is high both in male and female as increasing their age and increasing to their age the infection rate of HIV of male is increases where as female is decreases. So prevalence of HIV is an emerging...
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Posted by admin on Jul 1, 2013 in |
The present study describes antioxident effect of coriandrum sativum extract against lead nirtate induced toxicity in an experimental model, the fresh water snailand Bellamya bengalensis. The effect of snail was studied under five groups. Group A was maintained as control, Group B snails were exposed to chronic LC 50/10 doses of lead nitrate (6.9ppm) for 21 days, while group C snails were exposed to respective chronic concentrations of heavy metals with 5 ml/lit of extract from coriandrum sativum. Rates of O2 consumption from all groups were estimated after 7, 14 and 21 days. Snails from B groups were divided into two groups after 21 days exposure to heavy metals into D & E groups. Snails of D group were allowed to cure naturally while those of E were exposed to extract from coriandrum sativum (5 ml/lit) and their rates of O2 consumption were studied after 7, 14 and 21 days. Remarkable decrease in rate of O2 consumption was observed in lead exposed snails. The groups exposed to heavy metals...
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Posted by admin on Jul 1, 2013 in |
A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Pregabalin in pharmaceutical Tablet dosage form. The mobile phase consisted of 50:50 % (v/v) of Methanol & 10mM Ammonium Acetate (pH adjusted to 3.0 with glacial acetic acid) operated on isocratic mode. The flow rate is 0.7 ml/min. Chromatographic determination of Pregabalin was performed on Phenomenex C18 column (150 X 4.6 mm Id, ODS 2, 5µm). The wavelength of detection is 210 nm. The injection volume is 20µL. The retention time of Pregabalin is 3.39 ± 0.10 minutes. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, solution stability, ruggedness, and robustness. The influence of Acid, Alkaline, Oxidative Stress, Photolytic stress conditions on Pregabalin was studied. Results indicated that pregabalin is stable under the experimental conditions with a high baseline noise observed in alkaline medium. The proposed method has been successfully used for the estimation in tablet dosage...
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