Posted by admin on May 1, 2013 in |
Vernonia amygdalina, commonly called bitter leaf, is a perennial shrub that belongs to the family Asteraceae and grows throughout tropical Africa. Traditional medicine practitioners use the plant as in treatment of several disease conditions especially diabetes mellitus. In this research, the feasibility of tableting Vernonia amygdalina leaf was examined using three binders. Tableting of the leaf powder was done using wet granulation method. Various physicochemical parameters (angle of repose, bulk density, tapped density, Hausner’s ratio, Carr’s index, ash value etc.) were determined and found to be within normal limits. The strength of the tablets was assessed by crushing strength and friability of the tablets while the release properties were determined using disintegration and dissolution times. The result showed that the feasibility of tableting the leaves of Vernonia amygdalina is high, possible and promising and that gelatin is a better binder compared to maize starch and microcrystalline cellulose for tableting the leaf of Vernonia...
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Posted by admin on May 1, 2013 in |
Purpose of present research work was to develop mouth dissolving tablets of loperamide using superdisintegrants. Mouth dissolving tablets of loperamide were prepared by direct-compression method after incorporating superdisintegrants Ac-Di-Sol, Sodium Starch Glycolate (SSG), and Crosspovidone in different concentrations. Effect of superdisintegrants on disintegration and dissolution behavior of tablet in O.1 N HCl, phosphate buffer (pH 6.8) was evaluated. To address poor solubility of loperamide, solid dispersion using solvent evaporation techniques was prepared using hydrophilic carrier PEG4000, PVP K-30, and Urea. Improvement in dissolution of drug was observed in solid dispersion as compared to pure drug. The 100% drug release was obtained from the solid dispersion (prepared by solvent evaporation method) of Loperamide: PEG4000 (1:6) in 10 minute. Formulations were evaluated for Weight variation, hardness, and thickness, friability, wetting time uniformity of dispersion, drug content, disintegration time and in vitro dissolution studies. The drug content of all the formulations was within the acceptable limits of the United States Pharmacopoeia XXVII. Optimized formulation showed good release profile with maximum drug being...
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Posted by admin on May 1, 2013 in |
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous analysis of Paracetamol (PCM) and Tapentadol (TAP) in their combined dosage form. Method A, First order derivative method involves measurement of absorbance at 257.060 nm (for TAP) and 289.406 nm (for PCM) in first order derivative spectra. Method B, Second order derivative method involves measurement of absorbance at 238.391 nm (for TAP) and 279.381 nm (for PCM) in Second order derivative spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 1.0 to 5.0 μg/mL for Tapentadol and 6.5 to 32.5 μg/mL for Paracetamol with R square value greater than 0.997 (for Method A) and 0.998 (for Method B). Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for all methods and mean %RSD was found to be less than 2 for all the methods. The methods were successfully applied for estimation...
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Posted by admin on May 1, 2013 in |
Andrographolide is one of the main active principles in Andrographis paniculata .It exhibits enormous pharmacological effects. Due to this immense value it required more level in pharmaceutical to satisfy the global need. Because it present in a very low level in normal regenerated plant of Andrographis paniculata. Micro propagation is a proven method for efficient invitropropagation of medicinal plants and for commercial exploitation of valuable plant derived pharmaceuticals. Hence in this present study aimed that to enhance the andrographolide content through hairy root culture techniques and elicitation process. Hairy root cultures induced in Andrographis paniculata used for determining the effect of signal compound on andrographolide production. Methyl jasmonate and salicylic acid were used in varying concentration and introduced into the 10d old hairy root cultures. HPLC studies are revealed that methyl jasmonate at 100 mM concentration shows higher efficacy (5fold/25d) than other increasing concentrations of same. Salicylic acid shows 8 fold (100mM) enhancements than hairy root cultures...
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Posted by admin on May 1, 2013 in |
The antitumor activity of methanolic extract of Decalepis hamiltonii was evaluated using Dalton’s Lymphoma Ascites (DLA) tumor model in Swiss albino mice. Methanolic extract of Decalepis hamiltonii was administered at a dose of 200mg and 400mg/kg b.wt once daily for 14 days after 24h of tumor inoculation. Cyclophosphamide was used as a standard drug at a dose of 25mg/kg b.wt. The result showed that methanolic extract of Decalepis hamiltonii root increased the mean survival time and percentage increase in life span and also decreased the body weight of the DLA tumor bearing mice. Hematological studies reveal that increase in hemoglobin content and RBC count and decrease in WBC count was observed in methanolic extract of root of Decalepis hamiltonii and cyclophosphamide treated mice. Administration of methanolic extract of root of Decalepis hamiltonii and standard control significantly decrease the serum marker enzymes (aspartate transaminase, alanine transaminase) and protein level to nearly normal levels in DLA tumor bearing mice. The methanolic extract of root of Decalepis hamiltonii at a dose of...
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