Posted by admin on Apr 1, 2013 in |
A simple, specific, precise, and efficient method for the Simultaneous estimation of Metformin HCl and Voglibose tablet by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase was in a combination of acetonitrile: buffer pH- 6.5 in the ratio of 62:38. Optimized column is a stainless steel column packed with base octa decylsilyl silica gel of 250X4.6mm and at 254 nm wavelength for metformin and voglibose detection by Spectrofluorimeter and excitation wavelength at 350nm and emission wavelength at 430nm. In our study the validation of analytical method for determination of Metformin and voglibose tablet formulation was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH...
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Posted by admin on Apr 1, 2013 in |
In the present study, the in vitro antimicrobial and cytotoxic activity of root extract of Coccinia grandis were evaluated. Ethanolic crude extract with its various solvent soluble fractions of C. grandis showed significant antimicrobial activity by disc diffusion method on some gram positive and gram negative bacteria. The extracts exhibited 9-12 mm (approximately) of zone of inhibition comparing with standard ciprofloxacin (15-19 mm). In the brine shrimp lethality bioassay, the crude ethanolic extract showed significant cytotoxicity with LC50 of 2.496 mg/ml in comparison to vincristine sulphate (LC50 value 0.451 mg...
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Posted by admin on Apr 1, 2013 in |
Background: Self-medication is an important component of self-care. Though it is widely practiced globally, very few studies have evaluated its pattern and prevalence in dental students. Aim: The study was conducted to compare pattern of self-medication practices between junior and senior dental undergraduate students. Methods: This was a cross-sectional, anonymous, descriptive study with a six month illness recall that evaluated two groups of dental students- Group I: second year BDS students who were unexposed to pharmacology subject; Group 2: students pursuing internship (interns) having a sound knowledge of pharmacology. A questionnaire consisting of both open and close ended questions related to various aspects of self-medication was handed to the students. Consenting students anonymously filled the questionnaire. Comparison between the two groups was done by two- tailed Chi-square test. P values<0.05 were considered as statistically significant. Results: A total of 167 students participated in the study.90% respondents in group 1 (second year) and 94% respondents in group 2 (interns) practiced self-medication. Illnesses for which self-medication was practiced included fever (76%...
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Posted by admin on Apr 1, 2013 in |
Backround: The present study has been carried out to evaluate the radio-protective effects of Alstonia scholaris extract (ASE) on peripheral blood of mice. Materials & Methods: Male Swiss albino mice were selected from an inbred colony and assorted into 4 groups. Animals in Group I were administered with double distilled water (DDW), volume equal to ASE, by oral gavage to serve as vehicle treated control. Mice in Group II were administered orally ASE once daily at a dose of 100 mg/kg b. wt. / animal/day for 5 consecutive days. In Group III, animals were given DDW for 5 consecutive days then exposed to 2.5 Gy gamma radiation. Group IV mice were treated with ASE (as in Group-II), and were exposed to 2.5 Gy after half an hour of the last administration of ASE. Animals of each group were autopsied on 12 hrs, days 1, 3, 7, 15 & 30 post-treatment intervals for hematological as well as biochemical estimations in blood. Results: Total erythrocyte count, hemoglobin level, and hematocrit percentage...
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Posted by admin on Apr 1, 2013 in |
The present study was conducted to evaluate the analgesic and anti-inflammatory potential of the flowers of Ervatamia coronaria. The analgesic activity of the extract was assessed by the acetic acid induced writhing test. Aspirin was used as a standard drug. The anti-inflammatory activity was evaluated by carrageenan induced rat hind paw edema method. The initial paw volume was measured plethysmographically immediately before subplanter injection. The relative increase in the paw volume was measured in control, standard and treated groups, 4h after carrageenan injection. The results of acetic acid writhing test in mice showed a significant decrease in number of wriths in ethanolic extracts of flowers of E. coronaria, suggesting peripheral analgesic effect. In carrageenan induced rat hind paw method; it was found that ethanolic extract of the flowers of E. coronaria, at a dose of 400 mg/kg body weight significantly reduced the oedema volume, which was comparable to standard drug diclofenac sodium. The flowers of the plant exhibited significant analgesic and anti-inflammatory activity, thereby justifying their use in traditional...
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