Posted by admin on Feb 1, 2013 in |
Tuberculosis is an infection bacterial disease caused by Mycobactrium tuberculosis, which most commonly affects the lung, it can be treated with Anti-TB drug which are classified into first and second line drug classes where the treatment regimen consists of two phases: the initial phase and the continuation phase. Isoniazid is one of the most important first line drugs for the treatment of tuberculosis and several methods have been reported to describe the quantitative determination of isoniazid, include the derivatization of isoniazid with several aldehydes to form the corresponding Schiff’s base. The aim of this study is to find a new spectrophotometric method for isoniazid (INH) analysis that combined the previously mentioned derivatization (hydrazone formation) with microwaves synthesis in order to achieve more accurate, rapid and simple method. The use of the microwaves to accelerate and quantitatively complete the reaction between isoniazid and the aldehyde is the most important modification we suggest in comparison to other previously mentioned method. Seven isoniazid Schiff’s bases of several aldehydes subjected to our investigation...
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Posted by admin on Feb 1, 2013 in |
An ongoing, systematic process designed to maintain the appropriate and effective use of drugs is Drug Use Evaluation (DUE). It involves a comprehensive review of patient’s prescription and medication data before, during, and after dispensing in order to assure appropriate therapeutic decision making and positive patient outcomes. The aim of the present study is to conduct a prospective study on utilization pattern of antibiotics in the department of medical teaching hospital situated in North Karnataka to evaluate the rational use of antibiotics, by comparing with the standard Clinical guidelines. The study is a Prospective observational with a sample size of 250 patients conducted using structured data entry forms. In our study, 155 patients were male and 95 were female. The most commonly used class of antibiotics are Cephalosporins (31.2%) preceded by Fluoroquinolones and Azoles. Penicillin’s were also prescribed for most of the infectious diseases. The duration of hospital stay is for more than 7 dayswith prolonged use of antimicrobial agents which does not provide an additional therapeutic benefit while the...
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Posted by admin on Feb 1, 2013 in |
A High Performance Liquid Chromatographic method was developed for determination of Diclofenac Potassium in in-vivo study during bioavailability study. The chromatographic separation was conducted on Shimadzu Prominence LC 20 connected with PDA detector; using mixed column ODS/Cyano; ACE, (100 x 4.6 mm, 5 µm). The mobile phase was isocratic consisted of Methanol: 50 mM potassium dihydrogen phosphate buffer, in ratio of (50 : 50 v/v) and was delivered to the system at a flow rate of 1.5 ml/min, with an injection volume of 20 µl and the detection wavelength (λ max) was 280 nm. Diazepam was used as internal standard. All assays were performed at ambient conditions. The calibration curve of Diclofenac Potassium in plasma was linear with correlation coefficient (r2) = 0.9996; over a concentration range of 0.25 – 3 ug/ml. The retention times for Diclofenac Potassium and the internal standard (Diazepam) were found to be 5.95 and 7.90 minutes, respectively. The mean recovery was found to be 90.57%.The relative standard deviation (RSD) was found to be <...
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Posted by admin on Jan 1, 2013 in |
Dalbergia spinosa Roxb. (Leguminosae) is a mangrove plant native to Asia and enjoys number of traditional uses. This paper describes the investigation of antibacterial and cytotoxic activity of the ethanol extract of D. spinosa leaves. Antibacterial activity of the ethanol extract of D. spinosa leaves was tested by disc diffusion assay and broth macrodilution assay. The extract was also tested for brine shrimp lethality. The extract showed antibacterial activity against a wide range of Gram-positive and Gram-negative bacteria in both assays. The minimum inhibitory concentrations (MIC) against these bacteria were in the range of 250-500 μg/mL. The LC50 against brine shrimp nauplii was 84.14 μg/mL. The above data demonstrates that the plant has some important biological activities with sufficient potency and further studies can be performed to identify the active...
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Posted by admin on Jan 1, 2013 in |
Felodipine is a long-acting 1, 4-dihydropyridine calcium channel blocker, used to control hypertension by selective action on peripheral resistance. The conventional felodipine tablet gives a rather high peak and comparatively low trough levels, due to rapid absorption and distribution. More sustained plasma concentrations might thus produce a more even effect on blood pressure. The main aim of the study was to improve dissolution rate of the dosage form in a controlled manner over extended period of 24 hrs. Matrix tablets were prepared by direct compression method,using hydrophobic polymers like Glyceryl monostearate and Carnauba wax. The prepared formulations were evaluated for hardness, thickness, weight variation, friability and in-vitro dissolution studies. Among all the formulations F8 was selected as optimized formulation based on the evaluation parameters and in-vitro release profile of 100% drug release for 24 hrs. The FTIR and DSC results of optimized formulation showed no drug-excipient interaction. For optimized formulation(F8), the drug release mechanism was explored and explained by zero-order (r2=0.984), first-order (r2=0.947), Higuchi (r2=0.967) and Korsmayer-peppas (r2=0.982 &...
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