Posted by admin on Mar 1, 2013 in |
Endophytic fungi residing inside the medicinal plants are of gaining importance for their bioactive compounds. This present study is undertaken to isolate and identify the potential endophytic fungi from Moringa oleifera, a traditional medicinal plant. A total of 24 segments each 12 from leaf and stem were collected, surface sterilized and was inoculated on to Sabouraud Dextrose Agar (SDA) plates. Based on the macroscopic & microscopic features the fungal isolates were identified as Alternaria spp., Aspergillus spp. Bipolaris spp., Exosphiala spp., Nigrospora spp., and Penicillium spp. Many unidentified sterile mycelia forms were also found which were grouped under the class mycelial sterilia. The Colonization Frequency (CF) and Endophytic Infection Rate (EIR) were observed as 91.66% and 45.83% respectively. The results of this study suggest that traditional medicinal plants are a rich and reliable source of novel endophytic fungi. Further studies are required with regard to the screening of these endophytic fungi for the production of novel bioactive compounds which are medically important in the treatment of...
Read More
Posted by admin on Mar 1, 2013 in |
Background: Black tea is rich in polyphenols which are antioxidants. The most abundant polyphenolic compounds are flavonols and catechins. Black tea antioxidants showed protective actions against chronic diseases like cardiovascular diseases due to their antioxidant action. Objective: The present study was conducted to evaluate the cardioprotective effects of Camellia sinensis on isoproterenol induced myocardial infarction in Wistar albino rats. Myocardial infarction (MI) is a condition in which a part of the heart muscle suddenly loses its blood supply, so the myocardium gets destructed and becomes ischemic. Camellia sinensis is a species of plant from which leaves and leaf buds are taken to produce tea. Myocardial infarction was induced by subcutaneous administration of Isoproterenol. Isoproterenol shows positive inotropic and chronotropic effects on heart. Oxidative products of drug cause cytotoxicity and myocardial damage. Methods: Wistar albino rats were divided into 8 groups, each group containing 8 rats. The following treatment was given for 21 days. Group 1: NS 1mg/kg (orally). Group 2: NS+ISO (85 mg/kg, s.c.). Group 3: BTE 1 (4mg/kg,...
Read More
Posted by admin on Feb 1, 2013 in |
The present study was undertaken to evaluate and compare the antioxidant activity and brine shrimp lethality bioassay of different parts (leaf and bark) of the plant Acacia auriculiformis. DPPH radical scavenging and NO scavenging capacity were measured for the determination of antioxidant activity. Ethyl acetate fraction of bark was found to possess highest DPPH radical scavenging activity with IC50 value of 7.80 µg/ml followed by methanol extract of leaf (IC50 value of 7.95 µg/ml). The IC50 value of standard ascorbic acid was 33.77 µg/ml. Incase of NO scavenging activity, n-hexane fraction of root found to have highest scavenging activity with IC50 value of 1.75 µg/ml followed by ethyl acetate fraction of leaf (IC50 value of 3.35 µg/ml). The IC50 value of standard ascorbic acid was 71.06 µg/ml. When compared with other fractions, the methanol fraction of leaf and bark had highest cytotoxic activity with LC50 value of 0.55 and 0.79 µg/ml respectively. This was followed by ethyl acetate fraction of leaf with LC50 value of 0.95 µg/ml. The standard...
Read More
Posted by admin on Feb 1, 2013 in |
Antimicrobial activity of extracts leaves of Murraya koenigii (Curry) and Camellia sinensis (Tea) plants prepared in water, methanol and ethanol by cold maceration method were evaluated for their antimicrobial activity against Staphylococcus aureus (3) strains, Pseudomonas aeruginosa (3) and Candida species: Candida albicans (2 and one strain each of Candida tropicalis, and Candida parapsilosis. The plant materials were procured from Kangra District of Himachal Pradesh, India. Methanol and ethanol extracts of both the plants produced significant zones of inhibition against all the bacterial and fungal pathogen used in the study in the antimicrobial assay. However, aqueous extract did not show any significant activity. In this assay, the susceptibility of Staphylococcus aureus to Gentamicin and Ampicillin, Pseudomonas species to Ciprofloxacin and Candida species to Amphoterecin B was also assessed for comparative analysis. All the Staphylococcus strains were sensitive to Gentamicin but resistant to Ampicillin, but Ciprofloxacin had significant inhibitory effect against Pseudomonas strains except one, PA-39. All the strains of Candida species were resistant to Amphoterecin B. Phytochemical screening of...
Read More
Posted by admin on Feb 1, 2013 in |
In Siddha system of medicine, the two formulations Vedikara silasathu parpam and Nerunjil kudineer in combination is given for urinary tract infection. Inhibition in the synthesis of inflammatory mediators and prostaglandins owe to the anti- inflammatory and antinociceptive properties of the drugs. In this study, the two Siddha formulations VSP and NK in combination was evaluated for anti- inflammatory activity by means of carrageenan induced rat paw edema method and antinociceptive activity with tail flick method in Wistar albino rats. In the carrageenan induced paw edema technique, rats at the dosage of 500mg/kg/p.o of VSP and NK, significant (p<0.001) inhibition of inflammatory progression was observed than the control group. In the tail flick method, VSP and NK at dosage of 500 mg dose, increased the tail withdrawal time significantly (p<0.001) when compared to the control group. This study had established the significant anti- inflammatory and antinociceptive activity of VSP and...
Read More
