Posted by admin on Feb 1, 2013 in |
Decrease in blood glucose level test with the use of oral glucose tolerance method from Avocado leaves was carried out. Swiss Webster male mice were divided randomly into five groups. They were negative control, positive control (Glipizide 0.013 mg/20 g BW) and three test groups treated with dosage of avocado leaves ethanol extract 0.490 g/kg, 0.980 g/kg and 1.960 g /BW respectively. Experiment was begun with feeding the mice with the test solution followed by feeding glucose solution (1.5 g/kg BW) 30 minutes later. Blood glucose levels were assessed using glucometer kit, from zero to 3 hours, at ½ hourly interval. These results analysed by one way ANOVA showed there were significant difference (p<0.05) between Ethanol extract of avocado leaves treated with mice (0.490, 0.980 g/kg BW) and control groups.Furthermore, when Tukey’s test was performed, avocado treated mice (1.960g/kg BW) reduce glucose level to 64.27%. The effectiveness of this treatment was not significantly different to those treated with glipizide...
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Posted by admin on Feb 1, 2013 in |
Background– Breastfeeding and good nutrition for children are essential for achieving the Millennium Development Goals, particularly the goals relating to child survival. Even though, most mothers in Ethiopia breastfeed their babies, they do not always follow the recommendations of the “National Strategy for Infant and Young Child Feeding”. Objective– The aim of this study was to assess determinants of breastfeeding practices among mothers’ of children aged less than 24 months attending governmental maternal and child health clinics in Mekelle town. Methods– Institution based cross sectional study was carried out among five health facilities selected using simple random sampling technique. Data was collected by interviewer administered structured questionnaire and it was entered, cleaned and analyzed by using SPSS for windows version 16.0. The proportion was used to describe the results and it was presented in the form of figures, tables and texts. The binary logistic regression model was used to test the association between dependent and independent variables. Result– A total of 361 mothers with their index child were interviewed. ...
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Posted by admin on Feb 1, 2013 in |
Tuberculosis is an infection bacterial disease caused by Mycobactrium tuberculosis, which most commonly affects the lung, it can be treated with Anti-TB drug which are classified into first and second line drug classes where the treatment regimen consists of two phases: the initial phase and the continuation phase. Isoniazid is one of the most important first line drugs for the treatment of tuberculosis and several methods have been reported to describe the quantitative determination of isoniazid, include the derivatization of isoniazid with several aldehydes to form the corresponding Schiff’s base. The aim of this study is to find a new spectrophotometric method for isoniazid (INH) analysis that combined the previously mentioned derivatization (hydrazone formation) with microwaves synthesis in order to achieve more accurate, rapid and simple method. The use of the microwaves to accelerate and quantitatively complete the reaction between isoniazid and the aldehyde is the most important modification we suggest in comparison to other previously mentioned method. Seven isoniazid Schiff’s bases of several aldehydes subjected to our investigation...
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Posted by admin on Feb 1, 2013 in |
An ongoing, systematic process designed to maintain the appropriate and effective use of drugs is Drug Use Evaluation (DUE). It involves a comprehensive review of patient’s prescription and medication data before, during, and after dispensing in order to assure appropriate therapeutic decision making and positive patient outcomes. The aim of the present study is to conduct a prospective study on utilization pattern of antibiotics in the department of medical teaching hospital situated in North Karnataka to evaluate the rational use of antibiotics, by comparing with the standard Clinical guidelines. The study is a Prospective observational with a sample size of 250 patients conducted using structured data entry forms. In our study, 155 patients were male and 95 were female. The most commonly used class of antibiotics are Cephalosporins (31.2%) preceded by Fluoroquinolones and Azoles. Penicillin’s were also prescribed for most of the infectious diseases. The duration of hospital stay is for more than 7 dayswith prolonged use of antimicrobial agents which does not provide an additional therapeutic benefit while the...
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Posted by admin on Feb 1, 2013 in |
A High Performance Liquid Chromatographic method was developed for determination of Diclofenac Potassium in in-vivo study during bioavailability study. The chromatographic separation was conducted on Shimadzu Prominence LC 20 connected with PDA detector; using mixed column ODS/Cyano; ACE, (100 x 4.6 mm, 5 µm). The mobile phase was isocratic consisted of Methanol: 50 mM potassium dihydrogen phosphate buffer, in ratio of (50 : 50 v/v) and was delivered to the system at a flow rate of 1.5 ml/min, with an injection volume of 20 µl and the detection wavelength (λ max) was 280 nm. Diazepam was used as internal standard. All assays were performed at ambient conditions. The calibration curve of Diclofenac Potassium in plasma was linear with correlation coefficient (r2) = 0.9996; over a concentration range of 0.25 – 3 ug/ml. The retention times for Diclofenac Potassium and the internal standard (Diazepam) were found to be 5.95 and 7.90 minutes, respectively. The mean recovery was found to be 90.57%.The relative standard deviation (RSD) was found to be <...
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