Posted by admin on Dec 1, 2012 in |
The present study was undertaken to evaluate antioxidant, cytotoxic and antimicrobial activity of Sonneratia alba bark. The carbon tetrachloride, chloroform soluble partitionate of methanolic extract and crude methanolic extract showed significant antioxidant property using 1,1-diphenyl-2-pecrylhydrazyl(DPPH) scavenging assay ,of which chloroform partitionate and crude extract demonstrated highest activity with IC50 value of 12µg/ml and 14µg/ml respectively. In the brine shrimp lethality bioassay, LC50 values obtained from the best fit line slope were 0.812, 14.94, 0.831 and 3.288 µg/ml for standard (Vincristine sulphate), n-Hexane, carbon tetrachloride and chloroform soluble partitionate of methanolic extract respectively. The carbon tetrachloride soluble fraction revealed moderate activities against Bacillus cereus, Bacillus subtilis, Sarcina lutea, Pseudomonas aeruginosa and Shigella dysenteriae test...
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Posted by admin on Dec 1, 2012 in |
Plant kingdom is one of the important sources of the natural product and food. In present era many lifesaving, nutritive chemical components are isolated from different plants due to both medicinal and economical values. In India Ayurveda provides major information about medicinal plants, but there are number of plants which is not studied totally. Citrus maxima is a perennial shrub commonly known as Papanus, distributed through India. Present Pharmacognostic research study was performed on leaves of Citrus maxima. Macroscopic photographs of transverse sections (T.S.) of Citrus maxim leaves shown distinct presentation of arrangement of palisade cells, vascular bundles, oil globules, parenchymatous cell & trichomes. Microscopy of the leaf shows abundant anisocytic stomata both surfaces of leaf, presence of vascular bundle in xylem vessels. Thick, ovoid, rectangular epidermal cells were present. Uniserate, multicellular, thin walled, unlignified, covering trichomes were present. Spongy parenchyma cells were present. Calcium oxalate crystals were present in the parenchymatous cells. Starch grains were present except vascular bundle. Oil globules were present in leaf. Standardization of leaf...
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Posted by admin on Dec 1, 2012 in |
Dillenia indica L. commonly known as elephant apple is distributed in the sub-Himalayan tract of India. The fruits of D. indica collected from Bhalukpong area of Sonitpur district of Assam (India) were dried, milled and extracted sequentially by Accelerated Solvent Extractor using four different solvents with increasing polarity (hexane < ethyl acetate < methanol < 50% aqueous methanol). Qualitative phytochemical analysis was carried out according to standard procedures. Total phenolic content (TPC) of all the extracts were determined by Folin-Ciocalteu’s method using UV-Vis spectrophotometer. Free radical scavenging activity were determined by measuring the decrease in the visible absorbance of 2,2-diphenyl-1-picrylhydrazyl (DPPH) on addition of the plant extracts and the mean inhibitory concentration (IC50) values were determined by plotting nonlinear regression curve. L-Ascorbic acid and gallic acid were used as standard in DPPH assay and TPC respectively. Phytochemical analysis confirms the presence of phenolic compounds in methanolic extract. The highest phenolic content (59.99±2.21 mg/g) was recorded in 50 % aqueous methanolic extract which also shows highest antioxidant activity with IC50...
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Posted by admin on Dec 1, 2012 in |
Antimicrobial activity of compound (2) was investigated against pathogenic microorganisms: Staphylococcus aureus (Gram (+), Escherichia coli (Gram (-) and fungal strain, Candida albicans using the Well Diffusion and the Poison plate methods. Compound (2) was shown to inhibit pathogenic growth, and judging from the area of the zone of inhibition, the susceptibility of the microorganism against compound (2) follows the sequence: Staphylococcus aureus > Candida albicans > Escherichia coli. The area of zone of inhibition ranging from (4.64 mm2 to 6.47 mm2) and (4.64mm2 to 5.72 mm2) for the Well Diffusion and Poison plates respectively. Both methods indicate that the area of the zone of inhibition for compound (2) is more potent than reference antibiotics: Nystatin and...
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Posted by admin on Dec 1, 2012 in |
Background: Enzyme catalysis is intermediate between homogeneous and heterogeneous catalysis. In general, it is similar to inorganic heterogeneous catalysis and sometimes it is called micro heterogeneous catalysis. Efficient enzyme catalysis depends on high specific nature of enzyme, pH, temperature, optimum temperature, colloidal nature, activator or co-enzyme, inhibitors or poisons. Immobilized β-galatosidase show high activity towards both pure lactose and lactose in skim milk, and a better thermal stability also. Methods: This study was done in CDRI, Lucknow. In this study β-galatosidase was extracted by Kluyveromyces lactis and enzyme assay done by GOD/POD method. Partial purification of enzyme was done by precipitation with ammonium sulphate, DEAE-cellulose column chromatography followed by SDS-PAGE. Immobilization of K. lactis cells was done by Entrapment method in which we agarose beads, plyacrylamide beads and alginate beads were used to analyze the catalytic activity of β-galatosidase on different parameters. Results: In this study the effect of temperature, pH, and amino acids on normal β-galatosidase and on immobilized β-galatosidase were analyzed. Immobilized enzyme showed the decreasing conversion level...
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