Posted by admin on Oct 1, 2012 in |
Milk is considered as a highly nutritious and valuable human food, consumed by the people in a variety of different products. The present work is an effort to have a survey of number of bacteria present in different milk samples viz., Raw, Boiled, Pasteurized and Skimmed milk collected from local market of Haridwar and its adjoining areas by serial dilution method and the quality of milk assessed by MBRT. The highest mean count was detected in raw milk having 211±0.8 cfu/ml. Among the four types, boiled milk appeared as of best quality. The major microbial contaminants were found to be Bacillus sp, Staphylococcus sp, Streptococcus sp, E. coli and Micrococcus sp. During the present investigation the antibiotic sensitivity test was also performed against all the five genera isolated. It was found that inhibition zone of Chloramphenicol and Ofloxacin was maximum against Streptococcus sp. (24 mm) and Staphylococcus sp....
Read More
Posted by admin on Oct 1, 2012 in |
The main purpose of the present study was to develop matrix moderated transdermal patches of Pioglitazone HCl using various ratios of Ficus carica fruit mucilage. Physical parameters such as moisture content, moisture uptake, tensile strength, elongation and folding endurance were evaluated. The matrix type transdermal patches were prepared using Pioglitazone HCl with Ficus carica fruit mucilage by the solvent evaporation technique. The interactions between Pioglitazone Hcl and Ficus carica fruit mucilage and Pioglitazone HCl were performed. The transdermal patches were subjected to various physicochemical parameters like mechanical properties, permeation studies and skin irritation studies were performed. The prepared patches possessed satisfactory preformulary and formulary characteristics. In vitro permeation studies were performed using a Keshary-Chien diffusion cell across hairless Albino rat skin. The nonionic surfactants Span 80, Glycerin, Propylene glycol in the formulation played a key role as permeability enhancers. The patches were found to seemingly free of potentially hazardous skin irritation. The experimental result shows that the release of drug from the patch was delayed in controlled manner as...
Read More
Posted by admin on Oct 1, 2012 in |
A novel, simple, accurate, sensitive, reproducible, economical spectroscopic method was developed and validated for the determination of Mefenamic acid and Dicyclomine hydrochloride in combined dosage form. Three different analytical methods, Absorption correction method, Differential derivative method, Simultaneous equation method were developed for estimation of Dicyclomine hydrochloride(10mg) and Mefenamic acid (250mg) in tablet dosage form. wavelength for estimation was 223nm for Dicyclomine hydrochloride and 308.60nm for Mefenamic acid in absorption correction method. 211.60nm was Zero crossing point of Mefenamic acid and 308.80nm was Zero crossing point of Dicyclomine hydrochloride which can estimate in differential derivative method. Simultaneous equation method was developed in NaOH which was linear in the range of 1-6µg/ml for Dicyclomine hydrochloride and 25-150µg/ml for Mefenamic acid, the correlation coefficient obtained was nearer to one. The method was validated for linearity, accuracy and precision as per ICH guidelines. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage...
Read More
Posted by admin on Oct 1, 2012 in |
A simple, selective, precise and reliable HPTLC method of analysis has been developed and validated for estimation of 6-gingerol in extracts of dried rhizomes of Zingiber officinale and marketed formulations containing Ginger. The method employed TLC aluminium plates precoated with silica gel 60F 254 as stationary phase. The solvent system consisting of n-hexane:ether (40:60v/v). Densitometric analysis was carried out in absorbance mode at 280nm. The method was validated in terms of linearity, precision and accuracy. The LOD and LOQ were 60 and 100ng/spot respectively for 6-gingerol. The average percentage recovery of 6-gingerol was found to be 99.93%. The developed method can be successfully used for identification and quantification of 6-gingerol in extracts and formulations. The method is also suitable for rapid and simple authentication and comparison of differences among samples with identical plant resources but different geographic...
Read More
Posted by admin on Oct 1, 2012 in |
Salbutamol is a potent anti-asthmatic agent, is a short-acting β2-adrenergic receptor agonist act. In the present study, an attempt has been made to prepare fast dissolving tablets of Salbutamol in the oral cavity with enhanced dissolution rate and to achieve better patient compliance. The tablets were prepared with four superdisintegrants e.g., Sodium starch glycolate, Cross-povidone, Cross-carmellose sodium, Pregelatinized starch by direct compression method. The pre-compression parameters of mixed blend were examined for angle of repose, bulk density, tapped density, Compressibility index and Hausner’s ratio. The tablets were evaluated for hardness, friability, weight variation, disintegration time, dissolution rate, content uniformity and drug content. It was concluded that the fast dissolving tablets with proper hardness, rapidly disintegrate with enhanced dissolution can be made using selected superdisintegrants. Among all formulations, batch D4 was considered as best since it showed enhanced dissolution, which leads to improved bioavailability, improved effectiveness and hence better patient...
Read More
