Posted by admin on Nov 1, 2012 in |
The Diethyl ether fraction of the Methanolic extract of Annona senegalensis (Pers) stem bark was prepared in yield of (0.16%). Preparative Thin Layer Chromatography analysis of the fraction resulted in the identification of two spots with antimicrobial activity. Subsequent GC/MS analysis of these spots resulted in the identification of ten components (spot one) and fourteen components (spot two). The fraction showed significant antimicrobial activity against clinical isolates of E. coli, S. enteriditis and S....
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Posted by admin on Nov 1, 2012 in |
A rapid and simple HPLC method has been developed for the quantification of curcumin in PGPR inoculated curcuma longa plant. Treatment of PGPR such as Pseudomonas fluorescens and Bacillus megaterium improved the content of curcumin in rhizomes sample. The PGPR treated rhizomes were higher in curcumin content than the PGPR untreated plant rhizomes sample. Analysis was performed using a C18 column (250 X 4.6 mm) by isocratic elution with 50mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40:60) and detection at 420 nm and 340 nm using a photodiode array detector for curcumin respectively. The calibration plot was linear over the range studied (Curcumin: 100 – 3200ng/mL) with a correlation of 0.999. The method was also validated for the precision and recovery. Thus, the method is suitable for routine analysis of curcumin in PGPR inoculated curcuma longa plant sample 1, compared with PGPR uninoculated plant sample...
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Posted by admin on Nov 1, 2012 in |
Introduction: – The nutritionalstatusand health outcome of older persons in low-income countries including Ethiopia is inadequately documented. Logistical difficulties and the absence of simple, easy-to-handle assessment instruments and data-collection problems in this environment are evident. As a result, nutritional data for older adults are inadequate. The scanty, mainly hospital-based, data that are available are not comprehensive, and they fail to incorporate essential indicators of nutritional status. Objectives: – the aim of this study was to assess the nutrition status and major risk factors of hypertension among adults In Tigray, Ethiopia. Methods: – A case control study design was used to assess the nutritional status and major risk factors of hypertension among adults in Tigray, Ethiopia. The study was conducted from Dec, 2010 to October, 2011. The study populations were both adult men and women (age >=40) residing in Tigray region. The study was conducted in three randomly selected hospitals. Sample size was calculated using Epi info statcalc, a structured questionnaire was used. The data was handled confidentially, and entered...
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Posted by admin on Nov 1, 2012 in |
Couroupita guianensis (Lecythidaceae) , is a medicinal plant which is endowed with curative properties including anti- fungal, anti – biotic, anti – septic, analgesic, anti-malaria, stomach – ache, tooth – ache, scabies, gastritis, bleeding piles, dysentry and scorpion poison. The study deals with the preliminary phytochemical screening of the stem of Couroupita guianensis with various extracts such as petroleum ether, benzene, ethanol and water. This includes the powder characteristics studies, fluorescence studies and thin layer chromotgraphic studies of the stem powder. The qualitative analysis of some secondary metabolites, to ascertain medicinal claims of this widely used medicinal plant. The results showed that the moderate presence of alkaloids, flavonoids, steroids, amino acids, phenols and...
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Posted by admin on Nov 1, 2012 in |
Clinacanthus nutans Lindau (Family: Acanthaceae) has attracted public interest recently due to its high medicinal values for the treatment of cancer, inflammation and various skin problems. This study was aimed to determine the oral LD50 value of the methanol leaves extract of C. nutans and identify the targeted organs in mice. This acute oral toxicity study was conducted in accordance to OECD 423 guidelines by using male Swiss albino mice weighing 25-35 g. First group was served as control group which received distilled water (vehicle) while second and third group were orally treated with single daily dose of 0.9 g/kg and 1.8 g/kg of methanol leaves extract of C. nutans, respectively. All the animals were closely observed for 14 days. Body weight for each mouse was recorded at day-0, day-3, day-7 and day-14. Relative organ weights for liver, kidney, spleen, lung and heart were also determined. All the results were presented as mean ± standard deviation and analyzed using Dunnett’s Test after ANOVA test. From the results obtained, no...
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