Posted by admin on Nov 1, 2012 in |
The study describes a validated reverse-phase HPLC method for the simultaneous estimation of paracetamol and naproxen in bulk and in tablet formulation. The proposed RP-HPLC method utilizes Eclipse XDB C18 column (150 ×4.6 mm i.d., 5 µm), optimum mobile phase consisted of gradient run of initial ratio of water (pH-2.5 adjusted with orthophosphoric acid): acetonitrile (87:13) with the effluent flow rate of 1.0 ml/min, and UV detection wavelength 263 nm. The developed method was statistically validated for linearity, precision, robustness, ruggedness and specificity. The method was linear over the range of 5-80 µg/ml for paracetamol and 3-48µg/ml for naproxen. The mean recovery was 99.72% and 100.88% for paracetamol and naproxen respectively. The intermediate precision data obtained under different experimental setup was quite concurrent with less critical % RSD. The proposed method can be useful in the quality control of paracetamol and naproxen in bulk drug and drug...
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Posted by admin on Nov 1, 2012 in |
The paper describes the in-vitro antioxidant activity of seed oil extracted from seeds of Mesua ferrea L. The plant extract has been found to possess various pharmacological activities which have been proved scientifically based on the ethnomedical data. The in-vitro antioxidant activity of M. ferrea seed oil (MFSO) was evaluated. The EC50 values of free radical scavenging activity (10.25 µg/ml), ABTS+ (652.5025 µg/ml) discoloration assay and nitric oxide scavenging activities (13.17 25 µg/ml) and determination of total phenolic content (121.5 µg GAE/ml), total antioxidant content 241.25 µg GAE/ml and total flavonoids content (66 µg QE/ml) in MFSO indicated its potential antioxidant...
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Posted by admin on Nov 1, 2012 in |
Thermal power plants produce enormous quantity of pollutants such as fly ash as a by – product of combustion of coal or any pulverized fuel at higher temperature. Industrial effluents contain several heavy metals including cadmium distrusted the metabolic process of living organism due to easily dispersion and mobilization in environment. The eatables and applicable potential plants and vegetables altered by Cadmium and concern derivatives which directly and indirectly posing severe risks to human health which, extremely toxic even in low concentration, and will bioaccumulate in organisms and ecosystems. Interest has therefore risen in its bio hazardous potential. Present study deals on monitoring or current status of cadmium in certain plant and common vegetable species in singrauli region, resulted indication were observed an alarming situation in Singrauli region of...
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Posted by admin on Nov 1, 2012 in |
Binahong (Anredera cordifolia (Ten) Steenis) has been used as wound healing in traditional Indonesian medicine and it is relevant to develop dosage forms of binahong using formulation technology approach. The aim of this research was to find out wound healing gel formula of ethanolic extract of binahong. The factorial design method 3 factors and 2 levels were employed to achieve this study. Three factors used in this study were Carbopol, Na-CMC and Ca-alginate with low and high level for each factor. The wound healing gel of ethanolic extract of binahong was evaluated for their physical properties i.e viscosity, spreadability, extrudability and bioadhesive properties. The results of this research were carbopol and Ca-alginate affecting physical properties of wound healing gel of ethanolic extract of binahong. Na-CMC affected physical properties of the gel except bioadhesive properties. Carbopol provided the greatest contribution to viscosity, spreadability, extrudability and bioadhesive properties of the...
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Posted by admin on Nov 1, 2012 in |
Osteoarthritis (OA) is disabling condition leading to loss of articular cartilage. Etiology of OA is not clearly understood and diagnosis of early stages of OA is difficult due to lack of specific markers. Recent literature suggest that elevation of Myeloperoxidase (MPO) levels in synovial fluid in early stages of OA as compared to controls and late stage OA. Presence of MPO in blood and Urine samples of OA patients is still not studied. Present study is designed to explore the role of plasma and urine MPO levels in different stages of osteoarthritis. Based on Kellgren-Laurence (K.L.) Score, study subjects were divided into 4 groups as per 4 grades of severity of OA. These assignments were made by experienced joint surgeons and were based on radiographic & clinical findings. MPO levels in plasma and urine is estimated by ELISA technique. Levels of Plasma and Urine MPO are found to be significantly higher in case Grade 1, grade 2(Early Knee OA) & MPO level goes on decreasing in Grade 3 &...
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