Posted by admin on Sep 1, 2012 in |
Phosphatase serves several functions in plant metabolism including growth governance, phosphorous level control, starch breakdown etc. Alkaline phosphatases, acting at an alkaline pH 8, are a significant class of enzymes that catalyze release of phosphate esters especially. This enzyme study is so far limited only to animal source and partly to microbial sources, in terms of clinical research. Although it has been identified that plant as a source of this enzyme may be exploited, there always has been a challenge on the isolation and characterization of this enzyme and how pure it can be. This paper partly addresses the above problem, where the enzyme has been isolated from the seeds Dolichos lab-lab plant characterized and its purity was checked by HPLC. The purity obtained was 98% and the enzyme has been further analyzed for its activity on nucleic acids, which gave promising and positive...
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Posted by admin on Sep 1, 2012 in |
Cyperus rotundus L. is a medicinal herb widely used in the ancient Ayurvedic and Chinese traditional medicine. In the earlier investigations it has been primarily studied for its antioxidant properties. The present study explores the various phytochemicals present in the plant so as to substantiate its antioxidant property and also unravels the several phytoconstituents that contribute to its various medicinal...
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Posted by admin on Sep 1, 2012 in |
In the present study, the microspheres containing Verapamil hydrochloride xanthan complexes encoated with ethyl cellulose by emulsion solvent evaporation method. DSC shown absence of any new endothermic peak, disappearance of no shift of endothermic peak confirmed that there is no any interaction between drug, excipients and the drug was thermally stable. Drug polymer ratio was altered while the other formulation parameters were kept constant and percentage yield, incorporation efficiency, particle size and distribution of the microcapsules were analyzed. The microcapsules studied for effects of the increase polymer ratio in formulation. The dispersed phase viscosities were evaluated by comparing with variations in particle size and distribution of the microcapsules and the effect of the variation in polymer ratio on drug dissolution was evaluated. The percentage drug released at the end of 12th h (VXM2) was found be 95.95%. respectively. The mechanism of drug release may also be dissolution and diffusion mechanism controlled released formulation. The stability of Verapamil hydrochloride loaded microcapsules (VXM2) formulation was stored at 40oC±2oC/75% RH ±5% RH...
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Posted by admin on Sep 1, 2012 in |
The purpose of present investigation was to achieve successful delivery especially to colon using microbially triggered, osmotically controlled approach. Prednisolone was chosen model drug for treatment of inflammatory bowel disease (IBD). Prednisolone-β-cyclodextrin complex were prepared and phase solubility study was carried out. Cellulose acetate solution containing chitosan was used to prepare semi-permeable. Face centered central composite design was employed to study the effect on independent variables (concentration of sodium chloride, polyethylene glycol, and chitosan), % cumulative release and disintegration time. Both independent variables, the proportion of chitosan (X1) and proportion of NaCl and PEG (X2) had an influence on the % drug release in both the media (buffers and buffer with rat ceacal content). Formulations were evaluated for their Preformulation parameters and found within the specified limit. Formulation code F8 and S8 were the optimized batches with an in vitro release of 83.77 and 99.13 % respectively. The formulations containing PEG was found to be a promising drug delivery system better release...
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Posted by admin on Sep 1, 2012 in |
Oxidative stress has been implicated in the eiteology of diabetic neuropathy (DN) and anti-oxidants have shown to ameliorate this condition. Previous studies have indicated that early treatment with vitamin E acetate (VE) and methylcobalamin (MCA) attenuated surgery induced neuropathy in rats. The aim of this study was to test the effect of early co-administration of VE (50 mg/kg) and MCA (500µg/kg) (VEM) on the development of DN in laboratory rats. Male Wistar rats were divided into the three treatment groups, sham (non-diabetic, vehicle treated), diabetic rats (vehicle treated; (DU), and diabetic rats which received VEM treatment (DVEM). Diabetes was induced by single injection of alloxan monohydrate (120 mg/kg). Body weights of all subjects were noted daily and serum glucose levels (SGL) were measured at regular intervals. Behavioural assessment of thermal hyperalgesia (TH) was performed using the hot plate test and warm water tail immersion test. Finally, motor nerve conduction velocities (MNCV) were measured on day 45. Significant enhancements in SGL were noted for DU rats and this remained throughout...
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