Posted by admin on Nov 1, 2012 in |
The present study was designed to investigate the antidiabetic potential of different extracts of Berberis aristata bark, in alloxan induced diabetic male wister albino rats (150-250g), Diabetes was confirmed after 5 days of single intraperitoneally injection of alloxan (140 mg/kg of body wt.) in 12 hours previously fasted rats. Petroleum ether extract of Berberis aristata (PEBA) (100 & 400 mg/kg, body wt.) & Ethanolic extract of Berberis aristata (EEBA) (100 & 400 mg/kg, body wt.) and the standard drug glibenclamide (10 mg/kg, body wt.) were given orally in 0.5% Tween 80 after 5 days of alloxan treatment daily for 20 days, except the normal and diabetic control group (n=6). Blood was collected from the retro orbital sinus of the rats for glucose, total cholesterol and triglyceride level determination on 1st, 5th, 10th and 20th days. PEBA (100 & 400 mg/kg, body wt.) showed significant reduction in blood glucose level (266.3 ± 0.9700 to 118.1 ± 0.9375mg/dl & 271.3 ± 0.6983 to 111.6 ± 0.8072 mg/dl, respectively), total cholesterol (92.40±0.7711mg/dl...
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Posted by admin on Nov 1, 2012 in |
Punica granatum L. belongs to the family Punicaceae, which is originating from the Middle East, extending throughout the Mediterranean, eastward to China and India. Punica granatum has been used extensively as a traditional medicine in many countries for the treatment of dysentery, diarrhea, helminthiasis, acidosis, hemorrhage and respiratory pathologies. In addition, P. granatum is reported to have antioxidant, anti-atherosclerotic and antiviral properties. The dried pomegranate peels (20gms) were extracted using water (100ml) & ethanol (100 ml). The Ethanolic Extract of Punica granatum (EPG) was active against the tested bacteria. EPG demonstrated promising antibacterial activity against tested gram positive and gram negative bacteria. The E. coli and S. aureus shows more susceptibility to...
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Posted by admin on Nov 1, 2012 in |
A new series of 2, 5-disubstituted-1, 3, 4-oxadiazole derivatives were synthesized using appropriate methods & structures of the synthesized compounds were confirmed by IR,NMR & Mass spectral data.. These ligands were prefiltered for their drug likeness properties by Lipinski’s rule of 5 and it was found that all the ligands satisfied the rule of 5 for oral bioavailability.The prefiltered ligands were subjected for docking studies using integrated web server called docking server to investigate the interactions between the target compounds and the amino acid residues of the Glycogen synthase kinase-3β. The docking studies were done using auto dock between computationally designed 2,5-disubstituted 1, 3, 4-oxadiazole derivatives and Glycogen synthase kinase-3beta (GSK-3β) protein. The Calculated free energy of binding and estimated inhibition constants (Ki) were remarkable when compared with the standard GSK-3 inhibitors. It is calculated by the Lamarckian Genetic Algorithm (LGA). These values & the proposed interactions suggested that the 2, 5-disubstituted-1, 3, 4-oxadiazole derivatives are excellent inhibitors of...
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Posted by admin on Nov 1, 2012 in |
Some new series of 3-(4-substituted-phenyl)- 2-thioxo-2,3-dihydroquinazolin-4(1H)-one, 3-(4-substituted-phenyl)-2-(methylthio)quinazolin-4(3H)-one and 3-(4-substituted-phenyl)-2-hydrazinylquinazolin-4(3H)-one were synthesized by cyclization of 4-substituted-phenylcarbamodithioate with methyl anthranilate followed by reaction with dimethylsulphate and then prepared compound was refluxed with hydrazinehydrate respectively. The starting material 4-substituted-phenylcarbamodithioate was synthesized from 4-substituted-aniline. The title compounds were investigated for antibacterial and antifungal activity by disc diffusion technique. Compound IIa, IIb and IIIa showed moderate activity against B. subtilis, E. coli and A. niger as compared to standard drug. Compound IIIb and Iva exhibited good activity against A. niger. Compound IIIc and IVc was found to be most active compound against E. coli and moderately active against B. subtilis and A. niger, of the prepared...
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Posted by admin on Nov 1, 2012 in |
An accurate, sensitive and precise RP-HPLC –Fluorescence method has been developed and validated for the estimation of Carvedilol Phosphate (CP) from bulk drug and Pharmaceutical Dosage form. The separation was achieved by a Brownlee analytical C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Methanol : Buffer in proportion of 70:20:10v/v/v, buffer was 5mM Potassium Di-hydrogen Phosphate (pH 3.5 adjusted with Ortho Phosphoric Acid). The flow rate of mobile phase was 1.0ml/min and employing fluorescence detection with 280nm excitation and 340nm emission wavelengths. The retention time of Carvedilol Phosphate was 2.20 min.The calibration curve was found to be linear within the concentration range of 10ng/ml to 60ng/ml. The regression data for calibration curve shows good linear relationship with r2 = 0.990. The method was validated in accordance with the requirements of ICH guidelines. Moreover, the proposed analytical method was applied to monitor the formulation commercially...
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