Posted by admin on Aug 1, 2012 in |
The aim of the study is to observe the cytotoxic activitiy of methanolic crude extracts of root of Justica adhatoda a plant belonging to the family Acanthaceae. The root of Justica adhatoda was extracted with organic solvent and the extracts were used for the observation of cytotoxic activitiy. Crude extracts (n-hexane, ethyl acetate and chloroform soluble fraction) of Justica adhatoda, were screened for cytotoxic activity using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it can be well predicted that n-hexane, ethyl acetate and chloroform soluble fraction of methanolic crude extracts possess cytotoxic principles (with LC50 1.129mg/ml, LC50 1.402 mg/ml and LC50 2.130 mg/ml respectively) comparison with positive control vincristine sulphate (with LC50 0.563...
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Posted by admin on Aug 1, 2012 in |
A simple, economic, selective, precise, and accurate High Performance liquid Chromatographic method for the analysis of Doxazosin in bulk drug and pharmaceutical formulations was developed and validated in the present study. The mobile phase consists of a mixture of Methanol and Potassium Dihydrogen Orthophosphate in the proportion 60: 40. And adjust the pH to 5.0 ± 0.05 with sodium hydroxide solution. This was found to give a sharp peak of Doxazosin at a retention time of 4.484 min. HPLC analysis of Doxazosin was carried out at a wavelength of 251nm with a flow rate of 1.0mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 50 ppm to 150 ppm. The linear regression equation was y =28.34x-16.91. The developed method was employed with a high degree of precision and accuracy for the analysis of Doxazosin. The developed method was validated for accuracy, precision, robustness, detection and quantification limits as per the ICH guidelines. ...
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Posted by admin on Aug 1, 2012 in |
QSAR study of lipid peroxidation (LPO) inhibitory activity of 18 flavonoids belonging to four classes namely flavones, isoflavones, flavonols and flavanones has been made with the help of PM3 calculations and geometry optimizations using CAChe software. Quantum-chemical descriptors were calculated by the same software using MOPAC 2002 engine. Two quantum-chemical descriptors, molar volume (M.V.) and dipole moment (μ) and two new indicator descriptors of electron densities on carbon and oxygen atoms are used for QSAR analysis. The results are highly encouraging as average values of correlation and cross validation coefficient are 0.794 and 0.754. Four QSAR models have high predictive power while one model has average predictability. These models can successfully predict the antioxidant activity of any newly discovered flavonoid which can later be tested in laboratory....
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Posted by admin on Aug 1, 2012 in |
The method for the simultaneous estimation of Cinnarizine and Domperidone maleate from tablet dosage form has been developed, based on simultaneous equation method at two selected wavelength 254nm and 284nm respectively, and also absorbance ratio method at two selected wavelengths 274.0nm (Iso-absorptive point) and 254.0nm (λmax of cinnarizine). The linearity was obtained in the concentration range of 5-20μg/ml and 5-20μg/ml for cinnarizine and Domperidone maleate respectively. These methods are simple, accurate and results of analysis have been validated statistically and by recovery...
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Posted by admin on Aug 1, 2012 in |
The aim of present investigation is to study the effect of oral administration of drug acetylsalicylic acid (ASA, aspirin) on female albino rat, Rattus norvegicus. The female rats (n=24) were allocated into 2 groups as control (n=12) and treated (n=12). The treated rats were given oral dose of aspirin 100 mg/ kg body weight for 15 days (n=6) and 30 days (n=6). Tissues enzymological estimations such as ACP (Acid Phosphatase) and ALP (Alkaline Phosphatase) along with histopathological studies of reproductive organs (ovary and uterus) were done. The present investigation indicated that, aspirin significantly increased ACP and ALP content in reproductive tissues and also caused significant histopathological variations in reproductive organs such as ovary and uterus by modulating certain enzyme metabolism in female albino...
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