Posted by admin on Oct 1, 2012 in |
The objective of this study was to develop simple, precise, accurate, reproducible and economical vireodt’s method for simultaneous estimation of mesalamine (MSM) and prednisolone (PRD) in combined oral dosage form. The method involved measurement of absorbance at two wavelengths, 332nm and 246nm, λmax of MSM and PRD, respectively in phosphate buffer (pH 7.4) with dimethyl formamide (DMF) as cosolvent. The linearity was obtained in the concentration range of 5-50 µg/ml and 2-20 µg/ml for MSM and PRD, respectively. The average percent recovery of MSM and PRD was found to be 99.19+0.78% and 99.71+0.82%, respectively. The accuracy and precision were determined and recovery studies confirmed the accuracy of the developed method that was carried out following the International Conference on Harmonization (ICH) guidelines. The recovery study was carried out by standard addition method. The proposed method was found to be rapid, specific, precise, accurate, and reproducible and can be successfully applied for the routine analysis of MSM and PRD in pharmaceutical dosage...
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Posted by admin on Oct 1, 2012 in |
The course on “Herbal Medicines, Nutraceuticals and Dietary Supplements” has been implemented among the new courses within the 2007 curriculum of the Pharmacy Program, School of Food, Biochemical and Pharmaceutical Sciences at the Catholic University of Cuyo, located in San Juan Province, Argentina. The main objective of the course is to provide students with the necessary tools to acquire knowledge on new concepts of nutritional therapies, functional foods and dietary supplements. All of which are meant to revert or prevent unfavorable health conditions. This paper describes the methodology used in the development of the course, the minimum contents exposed, experience and teacher evaluation during the years 2009 and...
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Posted by admin on Sep 1, 2012 in |
NLC are innovative vehicles introduced to overcome some limitations of SLN. Particularly, NLC, composed of a solid lipid and a certain content of liquid lipid (oil), show improved drug entrapment efficiency and an increased stability during storage with respect to SLN. Furthermore, NLC, maintaining their solid state, can control the API release from the matrix. To formulate Nanostructure lipid carriers of an anti-inflammatory drug Betamethasone Dipropionate. To conduct in vitro Diffusion studies using dialysis membrane. To Ex vivo permeation study using rat skin. To conduct stability studies at 4°C and room temperature and to evaluate entrapment efficiency at various time...
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Posted by admin on Sep 1, 2012 in |
This study was designed to investigate the ameliorative effect effect of Hygrophila auriculata (H. auriculata) on lipid peroxidation (LPO) in N-nitrosodiethylamine induced Hepatocellular Carcinoma (HCC) in rats. Experimental rats were divided into different groups: normal, N-Nitrosodiethylamine induced hepatocellular Carcinoma bearing rats, H. auriculata treated hepatocellular carcinoma bearing rats, (200 mg/kg body weight, doses for 28 days), Animals treated with plant extract alone for 28 days. After the treatment period of 28th day , effect of H.auriculata on LPO in serum , liver and kidney of control and experimental animals were assayed and compared. Under basal and in the presence of inducers (H2O2), the levels of LPO were increased significantly (p<0.001) in group II cancer bearing animals where as the levels of LPO in the presence of ascorbic acid and FeSO4, were not statistically different. In group III plant extract treated animals, significant decrease on LPO was observed. However, the changes in the above parameters were comparable with control.Thus, methanolic extract of H. auriculata ameliorated the LPO which is associated...
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Posted by admin on Sep 1, 2012 in |
Cefuroxime is a 2nd-generation cephalosporin and Sulbactam is a β-Lactamase inhibitor. The combination formulation is used for the treatment of lower respiratory tract infection. Two new, simple, accurate and precise UV spectrophotometric methods have been developed and validated for the simultaneous determination of Cefuroxime Sodium (CEF) and Sulbactam Sodium (SUL) in their combined dosage forms. First method is based on simultaneous estimation of Cefuroxime at 279nm and Sulbactam at 259 nm, while other Q‐absorption Ratio method using two wavelengths, 259nm (λmax of SUL) and 272nm (Isoabsorptive point). 0.01 N NaOH was the solvent used in all methods. Cefuroxime Sodium showed linearity in the range of 8-32μg/mL and Sulbactam sodium showed linearity in the range of 4-16μg/mL in all the methods. All methods were validated statistically and recovery studies were carried out. All methods were found to be accurate, precise and reproducible. These methods were applied to the assay of the drugs in marketed formulation, which were found in the range of 98.0% to 100.0% of the labelled value for...
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