Posted by admin on Aug 1, 2012 in |
Pyrus pashia Buch.-Ham. ex D. Don is distributed in the Himalayan region with great ethnic uses. Leaves of P. pashia are consumed as butter tea beverages by the Monpa community of Tawang, Arunachal Pradesh (India). The pulverized leaves were extracted using accelerated solvent extractor (ASE) in different organic solvents with increasing polarity. The leaf extracts of P. pashia were evaluated for the free radical scavenging activity by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay and total phenolic content (TPC) were determined by Folin Ciocalteu’s method. The methanolic and water extracts shows strong free radical scavenging activity with IC50 values of 10.81±0.44 µg/ml and 11.57±0.36 µg/ml respectively. The methanolic extract has the highest TPC value of 351.16±0.43 mg/g. The study highlighted the antioxidant potentiality of Pyrus pashia Buch.-Ham. ex D. Donleaves and confirms it as a healthy beverage taken by the Monpa...
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Posted by admin on Aug 1, 2012 in |
Three simple sensitive, rapid, accurate and economical methods were developed for simultaneous estimation of Ciprofloxacin & Ornidazole in bulk and its pharmaceutical formulations. First method was based on the simultaneous equation, second method was based on Q-Analysis (Absorbance ratio method) and third method was based on multicomponent mode. The absorption maxima for Ciprofloxacin and Ornidazole were 272 nm and 319 nm in 0.1 N NaOH respectively. The iso-absorptive point for Ciprofloxacin Hydrochloride and Ornidazole was 292 nm. The calibration curve was linear over the concentration range 2.5 to 22.5 µg/ml of Ciprofloxacin & Ornidazole. The methods had been validated statistically and by recovery study. It can be used in the simultaneous estimation of Ciprofloxacin and Ornidazole in bulk drugs and its pharmaceutical preparations in a routine...
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Posted by admin on Aug 1, 2012 in |
The common usage of Galium spp. in the Ethiopian traditional medicine, as described in few documentations, are generally related to diseases associated with the nervous system, the immune system, infections and inflammation. Preliminary phytochemical and antimicrobial screening on extracts of the aerial parts of Galium spurium (subspecies africanum) was done in order to partly rationalize some of the claimed traditional uses of the plant. Results of the preliminary antimicrobial screening on extracts of G. spurium showed only weak antibacterial activity. The preliminary phytochemical screening revealed the presence of flavonoids and phenolics compounds. As phenolics and flavonoids are known to display multiple health benefits, the claimed traditional uses of the plant could be justified more through the possible pharmacological effects of these components than its weak antibacterial...
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Posted by admin on Aug 1, 2012 in |
The aim of the study is to observe the cytotoxic activitiy of methanolic crude extracts of root of Justica adhatoda a plant belonging to the family Acanthaceae. The root of Justica adhatoda was extracted with organic solvent and the extracts were used for the observation of cytotoxic activitiy. Crude extracts (n-hexane, ethyl acetate and chloroform soluble fraction) of Justica adhatoda, were screened for cytotoxic activity using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it can be well predicted that n-hexane, ethyl acetate and chloroform soluble fraction of methanolic crude extracts possess cytotoxic principles (with LC50 1.129mg/ml, LC50 1.402 mg/ml and LC50 2.130 mg/ml respectively) comparison with positive control vincristine sulphate (with LC50 0.563...
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Posted by admin on Aug 1, 2012 in |
A simple, economic, selective, precise, and accurate High Performance liquid Chromatographic method for the analysis of Doxazosin in bulk drug and pharmaceutical formulations was developed and validated in the present study. The mobile phase consists of a mixture of Methanol and Potassium Dihydrogen Orthophosphate in the proportion 60: 40. And adjust the pH to 5.0 ± 0.05 with sodium hydroxide solution. This was found to give a sharp peak of Doxazosin at a retention time of 4.484 min. HPLC analysis of Doxazosin was carried out at a wavelength of 251nm with a flow rate of 1.0mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 50 ppm to 150 ppm. The linear regression equation was y =28.34x-16.91. The developed method was employed with a high degree of precision and accuracy for the analysis of Doxazosin. The developed method was validated for accuracy, precision, robustness, detection and quantification limits as per the ICH guidelines. ...
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