Posted by admin on Jul 1, 2012 in |
The voltammetric behavior of olopatadine was investigated at hanging mercury drop electrode using cyclic voltammetry and differential pulse voltammetry. The drug under study exhibited a single, well-defined reduction peak owing to the reduction of carbon-carbon double bond. The electrode and reaction conditions which yielded maximum peak current were established using differential pulse voltammetry. A linear relationship was observed between the peak current and the concentration of olopatadine over the range 1.0 x10-8 M to 4.0 x10-7 M. The limits of detection and limits of quantitation were found to be 1.92 ng/mL and 6.34 ng/mL respectively. The proposed method was successfully applied for the determination of olopatadine in pharmaceutical formulations without interference from...
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Posted by admin on Jul 1, 2012 in |
A simple, accurate, precise analytical method has been developed for the simultaneous estimation of ritonavir and lopinavir in pure bulk drug and in combined tablet dosage form by UV spectrophotometry by first order derivative method. The standard solutions of ritonavir and lopinavir were prepared in acetonitrile followed by further required with the same solvent. The solution containing ritonavir and lopinavir (20µg/mL and 80µg/mL) were scanned between 400 nm to 200 nm and from the overlain first order derivative graph it appeared that ritonavir showed zero crossing at 278.10 nm while lopinavir showed zero crossing at 246.70 nm. At zero crossing point of ritonavir (278.10 nm), lopinavir showed a measurable derivative absorbance where as at the zero crossing point of lopinavir (246.70 nm), ritonavir showed appreciable derivative absorbance value. Thus both the drugs do not interfere in the quantitation of one another. Calibration graphs showed linearity at the concentration ranges from 5-30 mg/ ml by ritonavir and from 20-120 mg/ ml by lopinavir. Corresponding regression equations of both the drugs...
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Posted by admin on Jul 1, 2012 in |
Process Validation is a very common term in the pharmaceutical industry. But it involves series of activities carried out in order to have the assurance that the desired quality products are manufactured. The manufacturing process need to be controlled and for this one should have sound knowledge and understanding regarding the process as well as the product. Each and every step should be scientifically planned and conducted and documented appropriately in order to have an effective and efficient program. So here we discuss the Prospective Process validation of the Sotalol Hydrochloride 40 mg tablets, the critical process parameters involved in the manufacturing process and the consistency in the results of the three consecutive...
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Posted by admin on Jul 1, 2012 in |
This research manuscript describes simple, sensitive, accurate, precise and repeatable UV spectroscopic method for the simultaneous determination of Metronidazole (MET) and Ofloxacin (OFL) in suspension dosage form). Metronidazole has absorbance maxima at 318.0 nm and Ofloxacin has absorbance maxima at 294 nm in Methanol and Water (50:50) solvent. The linearity was obtained in the concentration range of 1-13 μg/ml for Metronidazole and 1-13 µg/ml for Ofloxacin with mean accuracies 99.73 ± 0.05 and 99.13 ± 0.41 for Metronidazole and Ofloxacin, respectively. This paper presents a useful UV spectroscopic method for validating equipment cleaning procedures and verifying cleaning in a pharmaceutical plant. The study summarizes the initial steps that should be taken into account and focuses particularly on the solutions to some of the most critical considerations (e.g., detection and quantification limits, recovery). Cleaning validation is the process of assuring that cleaning procedures effectively remove the residue from manufacturing equipment/facilities below a predetermined level. This is necessary to assure the quality of future products using the equipment, to prevent cross-contamination,...
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Posted by admin on Jul 1, 2012 in |
A Simple, Sensitive, Precise, and specific reverse phase high performance liquid chromatographic method has been developed for the determination of lymecycline in pharmaceutical Dosage Forms. Chromatographic separation was achieved on a PLRP-S (250×4.6 mm), 8.0 µm make: Varian column with a 11.5:10:20:1:57.5 mixture of 2-Methyl-2-propanol,3.5% w/v di-potassium hydrogen phosphate, 1.0% w/v Tetra butyl ammonium hydrogen sulphate, 4.0% w/v di -sodium edetate and JT Baker water as mobile phase, detection was at 254 nm. Response was a linear function of concentration in the range 5-0.02 mg/L for lymecycline; correlation coefficient was 0.9998, respectively. LOD and LOQ for lymecycline were found 0.02 mg/L and 0.05 mg/L. Accuracy (recoveries 95-97%) and reproducibility were found to...
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