Posted by admin on Apr 1, 2012 in |
Antimicrobial and cytotoxic activity of petroleum ether, ethyl acetate and methanol extracts from Juniperus indica Bertolwere evaluated. The extracts screened for their antibacterial activity against human pathogenic bacterial strains, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. All the fractions presented antimicrobial activity with variable strengths to all of the tested microorganisms. Phytochemical screening revealed the presence of terpenoids, flavanoids and tannins as main phytochemical groups. Cytotoxic activity was evaluated by using brime shrimp lethality test. LC50 value for the methanolic, ethyl acetate, and petroleum ether extracts were calculated at 550 µg/ml, 380 µg/ml and 140 µg/ml...
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Posted by admin on Apr 1, 2012 in |
A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical...
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Posted by admin on Apr 1, 2012 in |
Adventitious bud regeneration from internode explants of Mentha viridis L. was achieved shoots from nodal segments grown in vitro, were cut in to pieces and uses as sources of explants. Organogenesis was induced from internodal explants cultured on Murashige and Skoog (MS) medium containing different combinations of 6, Benzylaminopurine (BAP) and Kn under 16 hr photoperiod and at a temperature of 22 ±2 Co. The types of explants markedly influenced organogenesis and growth of the regenerated shoots. The regeneration frequencies were high in intermodal explants. The percentage of responsive in internodal explants producing from 20 to more than 91. BAP 3.5 mg/l induced the highest regeneration rate 91% and also get results of KN 3mg/l, regeneration rate 81 after 20d of culture. Yielding 36.7±1.14 shoots per responsive internode explants. The regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for 30d. Finally the elongated shoots were separated and transferred on to MS medium supplemented with (IBA, 1.5 mg/l). Rooted plantlets (90%)...
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Posted by admin on Apr 1, 2012 in |
Indapamide, a low-dose thiazide-type diuretic, is used for the treatment of essential hypertension. In this study, we developed an indapamide sustained release formulation using Methocel K15M CR and Methocel K100M LVCR, starch 1500, talc and magnesium stearate considering technical feasibility and performed a comparative study with the release pattern. The tablets showed sustained release curves at pH 6.8 phosphate buffer for up to 12 h. The granules showed satisfactory flow properties, compressibility index and drug content etc. All the tablets complied with pharmacopoeial specifications. The results of dissolution studies indicated that the formulations F-6 and F-8 could extend the drug release up to 12 h. The data obtained from the dissolution profiles were compared in the light of different kinetics models and the regression coefficients were also compared. Kinetic modeling of in vitro dissolution profiles revealed the drug release mechanism ranges from diffusion controlled or Fickian transport to anomalous type or non-Fickian transport, which was only dependent on the type and amount of polymer used. The drug release followed...
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Posted by admin on Apr 1, 2012 in |
Eleutherine plicata Herb., Iridaceae, is an herbaceous Iridaceae, vernacular marupazinho, largely used as tea, in the Amazonian popular phytotherapy, to treat diarrhoea alleged to amoeba infestation. To investigate the form of use of this medicinal plant andits alleged activity, and to perform a phytochemical analysis and an anti-amoebaean assay. Bulbs of the plant were dried, grinded and extracted in boiling water. After concentration the extract (AEEp) was analysed by LC-DAD and tested against trophozoitic forms of three strain of Entamoeba histolytica/Entamoeba dispar. A significant peak in the LC-DAD chromatogram of AEEp can be observed and attributed to isoeleutherine, a naphtoquinone also an antiamoebian activity was detected for this extract. Isoeleutherine would be one of the active substances responsible for the anti-amoebaean activity of Eleutherineplicata Herb., since naphtoquinone show anti-protozoal activity and the substance shows pro-oxidative properties. Noteworthy is the fact that the decoction shows 3.5mg dry residue/mL, 40% higher than the concentration of the extract used in the bioassay (2.5mg/mL). The detected antiamoebian activity for AEEp aligns to the...
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