Posted by admin on Jun 1, 2012 in |
Pseudomonas aeruginosa, is one among the few drug resistant organisms causing Urinary Tract Infection. Most strains of Pseudomonas aeruginosa are significantly more resistant, even in the absence of R plasmids, to many antimicrobial agents, including β-lactams, tetracycline, chloramphenicol, and fluoroquinolones, than most other gram-negative rods. This broad-range resistance has so far been assumed to be mainly due to the low permeability of the P. aeruginosa outer membrane. Mex-proteins are the multidrug exporter proteins that are significant in drug ejection, which is the main reason for drug resistance. This limits the therapeutic options. Inhibition of multi drug resistant efflux pumps can restore the activities of antimicrobial agents that are substrates for these proteins. Hence efforts are now taken to screen few medicinal plants, which are both economic and less toxic. Among the several plants screened, we have chosen the acetone extract of Elephantopus scaber from which we purified a new terpenoid for our study. Its structure was generated using CHEMSKETCH software. The novel terpenoid was evaluated as potent efflux inhibitor. In...
Read More
Posted by admin on Jun 1, 2012 in |
In this study, isolation, screening, characterization, antibiotic susceptibility tests, curing & Determination of B-complex vitamin production by potent probiotic Lactobacillus strains from different sources of meat was analysed. 250 grams of intestine parts of goat meat and beef each were transferred to MRS broth to screen the potent probiotic Lactobacillus strains. Initially 57 strains were isolated and confirmed by gram`s staining and catalase tests. After that, 51 strains selected for further biochemical tests to confirm all were Lactobacillus. Later, 41 strains were selected for antibiotic susceptibility tests by using three different classes of antibiotics viz., tetracycline, ampicilin, and streptomycin. Those strains which showed resistant to all three antibiotics used, further selected for curing of plasmid DNA and again antibiotic susceptibility tests was performed by the same antibiotics used before. Finally, only 5 strains were selected as potent probiotic Lactobacillus and unfortunately all strains from Beef intestine derived. Then, determination of B-Complex vitamin production of those selected 5 potent probiotic Lactobacillus by thin layer chromatographic method; Spectroflurophotometric method and RP-HPLC...
Read More
Posted by admin on Jun 1, 2012 in |
Mikania lindleyana is a plant widely distributed in Brazilian Amazonia, popularly known as “sucuriju” and largely used in folk medicine to treat inflammation, chronic ulcers and pain. In the present study, we identified the secondary metabolites of the aqueous extract of M. lindleyana (AEML) and investigate its effects on several models of inflammation and nociception in rodents. Phytochemical screening of the AEML showed the presence of saponins, proteins, amino acids, phenols, tannins, organic acids and flavonoids. Oral pretreatment of mice with AEML significantly (P < 0.05) reduced the abdominal constrictions evoked by acetic acid injection and the licking time in both first and second phases in the formalin test but has no significant effect on hot plate test. In rats, AEML inhibited the edema formation induced by carrageenan and croton oil while has no significant effects on the edema induced by dextran. AEML inhibited the carrageenan-induced neutrophil migration as well as the rolling and the adhesion of leukocytes. The present study shows for the first time that AEML displays...
Read More
Posted by admin on Jun 1, 2012 in |
Plants have been used in traditional medicine for several thousand years. India is perhaps the largest producer of medicinal herbs and is rightly called the “Botanical garden of the World”. Murraya koenigii Linn. commonly known as Meethi neem, belongs to the family Rutaceae. The curry tree is native to India and it is found almost everywhere in the Indian subcontinent excluding the higher levels of Himalayas. The effect of Murraya koenigii leaves aqueous extract on biophysical and biochemical parameters of wound were studied by dead space wound model in diabetic hyperlipidemic rats. In dead space wound model, animals treated with Murraya koenigii (oral administration of variable dosage level 200mg/kg, 300mg/kg and 400mg/kg) leaves aqueous extract showed significant increase in Wet & Dry granulations tissue weight (biophysical parameter) and hydroxyprolin content (biochemical parameter) when compared to the diabetic hyperlipidemic control group rats. In this study, very significant (p<0.001) result was found with 300mg/kg dose level because the effect was dose dependent up to 300mg equivalent of extract. The results suggested...
Read More
Posted by admin on Jun 1, 2012 in |
The in vitro dissolution studies of encapsulated sodium salicylate granules coated with beeswax is presented. The factors investigated were the effects of concentration, presence of a hydrophilic fatty material, polyethylene glycol 6000 (PEG 6000), and technique (pan-coating, fusion and granulation) on the sustained release of drug when coated with beeswax. Comparison of release rates was based on the use of a parameter, t70% (time for 70% of drug to be released). The greater the concentration of beeswax, ranging from 13.04 to 28.75%, the more prolonged the release. The presence of PEG 6000 at a concentration of 1:9 beeswax in the coating fluid significantly (p<0.05) increased the release rate, and at a concentration of 1:1 nullified the sustained release effect of beeswax. The t70% for the fusion, granulated and pan-coated batches were in the increasing order of 25min., 1hr.35min. and 2hr.45min,...
Read More
