Posted by admin on Jun 1, 2012 in |
A new, simple, precise, sensitive, accurate and reproducible Reverse phase HPLC method was developed and validated for the analysis of amlodipine in bulk dosage forms. The separation was conducted by using c-18 RP-HPLC coloumn, which was maintained at ambient temperature. The mobile phase consisting of Phosphate buffer and Acetonitrile (90:10v/v) was delivered at a rate of 1.5 ml/min. The analysis was detected by using UV detector at the wave length of 225nm.The method is validated for its specificity, precision, accuracy, linearity and robustness. The method was found to be linear over the concentration range 10-100 mg/ml (r2 =0.999). The retention time for amlodipine was found to be 3.34min. Limit of quantification of the method is 0.179mg/ml and limit of detection is 0.054...
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Posted by admin on Jun 1, 2012 in |
The aim of the study was to investigate the phytochemical compounds of Wattakaka volubilis ethanolic leaf extracts. The phytochemical compounds were screened by GC-MS method. The ethanolic leaf extracts presented 32 bioactive compounds. The identification of phytochemical compounds is based on the peak area, molecular weight and molecular formula. Diethyl phthalate C12H14O4 has peak area 37.31, 2-pentanone, 3, 3, 4, 4-tetramethyl C9H18O has peak area 17.58 and propane 1, 1, 3-trimethoxy C9H20O3 has peak area 10.20 and the results were...
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Posted by admin on Jun 1, 2012 in |
Cough is the most common symptom of respiratory diseases. When cough becomes serious, opioids are effective, but they have side effects like sedation, constipation. Therefore, there is a need to have effective antitussive agent which do not have respiratory depressant activity. The present study was carried out to evaluate antitussive activity of Glycyrrhiza glabra and Adhatoda vasica using a cough model induced by sulphur dioxide gas in mice. The effect of the ethanol extracts of Glycyrrhiza glabra and Adhatoda vasica on SO2 gas induced cough in experimental animals have very significant effects at the level of p<0.01 in inhibiting the cough reflex at a dose of 800 mg/kg and 200 mg/kg body wt. p.o., in comparison with the control group. Mice showed an inhibition of 35.62%, in cough on treatment with Glycyrrhiza glabra and 43.02% inhibitionon treatment withAdhatoda vasica within 60 min of the experiment. The antitussive activity of the extract was comparable to that of codeine sulphate (10, 15, 20 mg/kg body wt.), a standard anti-tussive agent. Codeine...
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Posted by admin on Jun 1, 2012 in |
Obesity is one of the main public health problems in developed countries. It is considered to be a risk factor associated with the genesis or development of major chronic diseases, including cardiovascular disease, diabetes, and cancer. Many botanicals may provide safe, natural, and cost-effective alternatives to synthetic drugs. The results of this study show promise for the potential use of these plant materials as a lipase inhibitor in the treatment of obesity. The present study tested the hypothesis that the selected herbs/extracts can inhibit the enzymatic activity of LPL in vitro. Based on the literature review Camellia sinensis leaves was selected for the present study. Methanolic (MCS) and aqueous (ACS) extracts of Camellia sinensis leaves were prepared by soxhlet and hot reflux extraction methods respectively. These extracts were then examined for their in-vitro lipase inhibitory activity at a concentration level of 0.5, 1 and 2 g/l and their percentage inhibitory effect were reported and statistically analyzed. Results of the in-vitro lipase activity reveals that percentage inhibition of MCS was...
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Posted by admin on Jun 1, 2012 in |
Flavonoids have been reported to have many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities along with strong antiproliferative effects. The fruits of Garcinia lancaefolia Roxb., family Cluseaceae , a plant endemic to Assam has been used in locally to treat many diseases for which it was selected for investigationg its antimicrobial properties. No scientific validation has been made so far on its phytochemical content and the antibacterial activity for which the methanolic and water extract of plant parts were investigated by Agar Well Diffusion method. Furthermore, our phytochemical studies indicated that methanolic extract of Garcinia lancaefolia flowers and fruits contains flavonoids, tannins, reducing sugar, steroid terpene and cardiac glycosides. The total phenolic and flavonoids content studies were quantified for all the plant parts. Antibacterial activities of the crude extract were screened against wound infection causing 5 human pathogens. The most susceptible bacteria were S. aureus, followed by beta hemolytic Streptococcus pyogens, while the most resistant bacteria were E. coli, followed by Bacillus subtilis. From the screening experiment, the crude...
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