Posted by admin on Jul 31, 2023 in |
This work aimed to develop and evaluate fast dissolving strip of Midodrine hydrochloride to overcome the conventional dosage form’s limitations and enhance dissolution rate, fast onset of action and improved patient compliance. Various batches of the mouth-dissolving film were prepared by using various grades of hydroxypropyl methylcellulose (HPMC) polymer and plasticizers such as Polyethylene Glycol 200, Polyethylene Glycol 400, Polyethylene Glycol 600 and Glycerin, used for the preparation of film by solvent casting method. The estimation was carried out using phosphate buffer pH 6.8 as a solvent using a double-beam spectrophotometer at 289nm. FTIR and DSC studies showed no interaction between drugs, polymers, or other additives. The prepared film was evaluated for transparency and surface texture, thickness, tensile strength, folding endurance, disintegration time, drug content, uniformity and in-vitro drug release study. Midodrine hydrochloride mouth dissolving film was prepared successfully by solvent casting method and that might be an alternative route of drug delivery system of conventional dosage forms with many...
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Posted by admin on Jul 31, 2023 in |
Background: Escherichia coli (E. coli) is one of the most common agents of bacterial infections, the emergence of multidrug resistance in E. coli has posed major threat to global health resulting in failure of treatment that leads to increased mortality and cost of healthcare facilities. Material and Methods: For this study, a total of 500 specimens like urine, blood, pus, stool, sputum and different body fluids received in the Clinical Microbiology Laboratory were processed and inoculated on culture plates (Blood agar, MacConkey agar, Cysteine Lactose Electrolyte Deficient Agar) and incubated at 37°C for 18-24 hours. After identification, E. coli was processed for Antibiotic susceptibility testing on Muller Hinton Agar using Kirby Baeur disk diffusion method. E. coli ATCC 25922 was used as reference strain. Results: Of 500 specimens received in clinical laboratory from different age groups, 180 were culture positive, of which 88 were E. coli isolates. 48(54.5%) E. coli isolates were obtained from female patients while 40(45.5%) were from male patients. Isolation of E. coli was highest in...
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Posted by admin on Jul 31, 2023 in |
The genus Ocimum, a member of the Lamiaceae family. Ocimum species are mostly composed of phenolics, tannins, saponins, flavonoids, volatile oils, etc. We have investigated into the antioxidant activity and total phenolics of the two species of Ocimum basilicum and Ocimum sanctum since they all have therapeutic characteristics. The results were compared to those of other common antioxidants like ascorbic acid and gallic acid. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, superoxide radical, hydroxyl radical, and lipid peroxidation can all be scavenged. These two plants’ leaves were extracted using an ethanol solvent. By Soxhlation, the ethanolic extract was obtained. Several concentrations (ranging from 50g to 500g) were employed in this investigation. The maximum percentage activity of DPPH (76.67%), Superoxide radical (60.72%), Hydroxyl radical (57.97%), and Lipid peroxidation (66.61%) were found at the 500 g concentration. Ocimum basilicum demonstrated greater percent inhibitory activity than Ocimum sanctum among these radicals evaluated on the two species. Ocimum basilicum was recommended, however, both Ocimum species had the highest IC50...
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Posted by admin on Jul 31, 2023 in |
The concept of quality by design (QbD), which entails understanding the essential components and their interaction effects by a desired set of tests, has lately gained relevance in developing analytical. To validate the simultaneous quantification of Ertugliflozin and Sitagliptin in bulk drugs and its pharmaceutical formulation, the present study discusses the development of the Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method by the QbD methodology employing the Design of Experiments. The three essential elements of the RP-HPLC method-Flow rate (ml/min), Mobile phase (%), and temperature (oC) are systematically explored in an effective experimental design that is provided. Statistical analysis tools were used to assess the significant impact of these parameters and their interactions with the response variables (Retention time and tailing factor). The 260nm optimized wavelength was chosen. Ertugliflozin and Sitagliptin were shown to have retention times of 3.897 and 2.527 minutes, respectively. Ertugliflozin and Sitagliptin can be estimated simultaneously using a linear method over the ranges of (1.875-11.25 g/ml) and (12.5-75 g/ml), respectively. The correlation coefficient R2 for...
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Posted by admin on Jul 31, 2023 in |
Cancer is a preminent cause of death worldwide after heart disease. Although there are many types of cancer treatments, depending on the type of cancer and its stages, due to their complexity and adverse cytotoxic effects, some novel targets at the molecular level that are cancer-specific have become the area of research for more than a decade. Some targets have been identified, and CDKs are among them. They play a pivotal role in the cell cycle. This has directed to developing of novel Cyclin-Dependent Kinase Inhibitors with a major emphasis on designing compounds that can effectively inhibit the cell cycle progression in cancer by inhibiting the CDKs selectively, such as abemaciclib. In this study, we have generated and evaluated some CDKIs. These compounds were designed to target CDK6 (PDB ID: 5L2S). The bio-affinity values and binding modes of all designed analogs were evaluated and the pharmacokinetic profile (Caco-permeability, efflux, fdp, vdss, bbb parameters) was also examined. The study exhibited significant findings and analogs 10c, 20a and 34b with docking...
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